Label-free electrochemical immunoassay of Bcl-2 protein expression on tumor cells

Chen, Lina; Luo, Yiqun; Liu, Tong; Yuan, Yanhong; Gu, Huoliang; Yang, Yong; Li, Ling; Tan, Liang

doi:10.1016/j.talanta.2014.09.047

Cited by 9 publications

(5 citation statements)

References 29 publications

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“…It was overexpressed in a lot of tumors and mediates survival signal in tumor cells. [30] In this study, the expression level of Bcl-2 was measured, and it showed that baicalein significantly down-regulated the expression of Bcl-2 in A549 cells (Figure 5E and F), which strongly supported that baicalein could promote apoptosis of A549 cells via reducing the activity of anti-apoptotic molecules Bcl-2.…”

Section: Confirming the Pharmacologic Activity And Investigating The ...supporting

confidence: 61%

Identifying Anti‐NSCLC Bioactive Compounds in Scutellaria via 2D NMR‐Based Metabolomic Analysis of Pharmacologically Classified Crude Extracts

Chen,

Li,

et al. 2024

Chemistry & Biodiversity

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We presented a strategy utilizing 2D NMR‐based metabolomic analysis of crude extracts, categorized by different pharmacological activities, to rapidly identify the primary bioactive components of TCM. It was applied to identify the potential bioactive components from Scutellaria crude extracts that exhibit anti‐non‐small cell lung cancer (anti‐NSCLC) activity. Four Scutellaria species were chosen as the study subjects because of their close phylogenetic relationship, but their crude extracts exhibit significantly different anti‐NSCLC activity. Cell proliferation assay was used to assess the anti‐NSCLC activity of four species of Scutellaria. 1H‐13C HSQC spectra were acquired for the chemical profiling of these crude extracts. Based on the pharmacological classification (PCA, OPLS‐DA and univariate hypothesis test) were performed to identify the bioactive constituents in Scutellaria associated with the anti‐NSCLC activity. As a result, three compounds, baicalein, wogonin and scutellarin were identified as bioactive compounds. The anti‐NSCLC activity of the three potential active compounds were further confirmed via cell proliferation assay. The mechanism of the anti‐NSCLC activity by these active constituents was further explored via flow cytometry and western blot analyses. This study demonstrated 2D NMR‐based metabolomic analysis of pharmacologically classified crude extracts to be an efficient approach to the identification of active components of herbal medicine.

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Section: Confirming the Pharmacologic Activity And Investigating The ...supporting

confidence: 61%

Identifying Anti‐NSCLC Bioactive Compounds in Scutellaria via 2D NMR‐Based Metabolomic Analysis of Pharmacologically Classified Crude Extracts

Chen,

Li,

et al. 2024

Chemistry & Biodiversity

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“…Tumor cells use several pathways to suppress apoptosis and acquire resistance to apoptotic agents, such as via expression of antiapoptotic proteins like Bcl-2 [ 54 ]. In fact, overexpression of Bcl-2 is a characteristic of drug-resistant tumor cells [ 55 ]. Drugs and microRNAs may regulate the Bcl-2 mediated apoptotic resistance [ 56 , 57 ].…”

Section: Xh Inhibits Tumor Cells Via Multiple Pathwaysmentioning

confidence: 99%

Review on the Applications and Molecular Mechanisms ofXihuangPill in Tumor Treatment

Guo

Lin

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Xihuang pill (XH) is a complementary and alternative medicine that has been used in traditional Chinese medicine (TCM) for the treatment of tumors since the 18th century. XH has clinical effects on non-Hodgkin lymphoma, breast cancer, gastric cancer, liver cancer, and bone metastasis. XH can also inhibit the growth of tumor cells and cancer stem cells, prevent tumor invasion and angiogenesis, and regulate the tumor microenvironment. XH is composed of Ru Xiang (olibanum), Mo Yao (Commiphora myrrha), She Xiang (Moschus), and Niu Huang (Calculus bovis). Some of the compounds found in these ingredients exert multiple antitumor effects and may synergize with the other ingredients. We aimed to summarize the clinical applications and molecular mechanisms of XH and its chemical composition. This review will provide potential new strategies and alternative perspectives for tumor treatments and basic research into complementary and alternative medicine.

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“…5 The detection of drug resistance in cancer cells has been approached by studying proteins, which are responsible for an increased number of drug efflux pumps when over expressed. Approaches in recent years include impedance spectroscopy and voltammetry, mostly through antigen/antibody modifications of electrodes 101,102 and specialized instrumentation, such as SECM to monitor drug resistance activity in living cells. [103][104][105][106][107] The most recent quantitative electrochemical approaches to measure drug resistance in living cells are discussed in the following section.…”

Section: Classesmentioning

confidence: 99%

“…3. 101 In this approach, GCEs were modified with L-Lysine through electropolymerization (PLL/GCE). Functionalized electrodes were then immersed in a glutaraldehyde aqueous solution for 1 h. Finally, these electrodes were further modified with a Bcl-2 antigen (Bcl-2Ag) and BSA to block nonspecific binding sites.…”

Section: Classesmentioning

confidence: 99%

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Review—Electrochemical Approaches and Advances towards the Detection of Drug Resistance

Islam

Luu

Kuss

2020

J. Electrochem. Soc.

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Drug resistance in bacteria and cancer is a growing problem that decreases drug treatment effectiveness and increases the severity of bacterial infections as well as cancer mortality. Due to their high sensitivity, low cost, and rapid analysis time, electrochemical methods have been increasingly employed to tackle this challenge throughout the last decade. This review covers literature on the electrochemical characterization of antibiotics and chemotherapeutic drugs, as well as advances in analyzing interactions between drug compounds and biological cells. Recent developments towards the quantitative detection of drug resistance in bacteria and cancer by electrochemistry are discussed, and the use of specialized electrochemical instrumentation, such as scanning electrochemical microscopy, is highlighted.

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Label-free electrochemical immunoassay of Bcl-2 protein expression on tumor cells

Cited by 9 publications

References 29 publications

Identifying Anti‐NSCLC Bioactive Compounds in Scutellaria via 2D NMR‐Based Metabolomic Analysis of Pharmacologically Classified Crude Extracts

Identifying Anti‐NSCLC Bioactive Compounds in Scutellaria via 2D NMR‐Based Metabolomic Analysis of Pharmacologically Classified Crude Extracts

Review on the Applications and Molecular Mechanisms ofXihuangPill in Tumor Treatment

Review—Electrochemical Approaches and Advances towards the Detection of Drug Resistance

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