Label-free interdigitated microelectrode based biosensors for bacterial biofilm growth monitoring using Petri dishes

Paredes, Jacobo; Becerro, S.; Arana, Sergio

doi:10.1016/j.mimet.2014.02.022

Cited by 61 publications

(34 citation statements)

References 33 publications

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“…Impedance characterization of S. epidermidis biofilm development on these devices allows for monitoring of bacterial growth as early as a few hours from the inoculation with high effectiveness. This same technique has since been extended to petri dishes [101] and 96-well plates [102]. Nonetheless, this method of detection does not provide any information on biofilm structure and physiology.…”

Section: Flow-based Systemsmentioning

confidence: 99%

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

Shi

Wang

et al. 2019

Biomaterials

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A B S T R A C TBacterial infections and antibiotic resistant bacteria have become a growing problem over the past decade. As a result, the Centers for Disease Control predict more deaths resulting from microorganisms than all cancers combined by 2050. Currently, many traditional models used to study bacterial infections fail to precisely replicate the in vivo bacterial environment. These models often fail to incorporate fluid flow, bio-mechanical cues, intercellular interactions, host-bacteria interactions, and even the simple inclusion of relevant physiological proteins in culture media. As a result of these inadequate models, there is often a poor correlation between in vitro and in vivo assays, limiting therapeutic potential. Thus, the urgency to establish in vitro and ex vivo systems to investigate the mechanisms underlying bacterial infections and to discover new-age therapeutics against bacterial infections is dire. In this review, we present an update of current in vitro and ex vivo models that are comprehensively changing the landscape of traditional microbiology assays. Further, we provide a comparative analysis of previous research on various established organ-disease models. Lastly, we provide insight on future techniques that may more accurately test new formulations to meet the growing demand of antibiotic resistant bacterial infections. Lack of adequate model systemsCommonly, a variety of inconsistent, in vitro and in vivo models have been progressively utilized for antibiotic/drug development and pathophysiological studies. These systems range from simple in vitro models using microtiter plate assays and flow cells to more complex in https://doi.

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Section: Flow-based Systemsmentioning

confidence: 99%

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

Shi

Wang

et al. 2019

Biomaterials

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“…Monitoring biofilm formation combining label-free biosensors and impedance spectroscopy measurements on the surface of electrodes is an alternative approach that has gained increasing interest, previously described using IDuEs [47,48] which combine both impedimetric and amperometric measurements [49]. In particular, much of the existing studies are based on two-or three-electrode measurements [50,51,[52][53][54] because of their simplicity and ability to detect and track the biofilm formation by the changes occurring at the surface electrode.…”

Section: Impedance Monitoring Of Bacterial Biofilm In Growth Mediamentioning

confidence: 99%

Simultaneous monitoring of Staphylococcus aureus growth in a multi-parametric microfluidic platform using microscopy and impedance spectroscopy

Estrada-Leypon

Moya

Guimerà‐Brunet

et al. 2015

Bioelectrochemistry

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“…At higher frequencies (from 10 kHz to 1 MHz, the resistance region, also evidenced by a phase angle close to 0 • ) no significant difference is observed, indicating that the detection of SM7 activity in the presence of lindane is only possible below 10 kHz. Some authors have assigned the impedance changes at low frequencies with bacterial adhesion to the electrodes [65,66] or with the growth of microorganisms [64,67,68]. These conditions do not apply to our case because SM7 spores are adhered during the impedimetric measurement; hence, the observed impedance change can only be attributed to the modification of the interface impedance due to metabolic activity of the bacteria.…”

Section: Resultsmentioning

confidence: 90%

A simple Streptomyces spore-based impedimetric biosensor to detect lindane pesticide

Rodriguez¹,

Benimeli²,

Madrid³

et al. 2015

Sensors and Actuators B: Chemical

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Label-free interdigitated microelectrode based biosensors for bacterial biofilm growth monitoring using Petri dishes

Cited by 61 publications

References 33 publications

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

Simultaneous monitoring of Staphylococcus aureus growth in a multi-parametric microfluidic platform using microscopy and impedance spectroscopy

A simple Streptomyces spore-based impedimetric biosensor to detect lindane pesticide

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