Label-free microarray-based detection of autoantibodies in human serum

Bucukovski, Joseph; Carter, Jared A.; Striemer, Christopher C.; Müllner, Stefan; Schulte-Pelkum, Johannes; Schulz‐Knappe, Peter; Miller, Benjamin L.

doi:10.1016/j.jim.2018.05.011

Cited by 8 publications

(10 citation statements)

References 24 publications

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“…In a previous work, we have demonstrated the utility of AIR as a tool for profiling the immune response to infection by and immunization against viral pathogens, including influenza , and SARS-CoV-2 . We have also used AIR to assess autoimmunity in Sjögrens disease and systemic lupus erythematosus . Here, we have provided the first demonstration of the use of bacterial antigens on the AIR platform.…”

Section: Discussionmentioning

confidence: 94%

“…Three different concentrations of the intra-chip correction probe (anti-fluorescein isothiocyanate, anti-FITC) were formulated from anti-FITC antibody (dialyzed for 4 h at 4 °C in mPBS to remove sodium azide) and mPBS. All probe solutions contained trehalose as an additive to improve spot morphology . Optimized print solutions used for the production of all arrays are shown in Table .…”

Section: Methodsmentioning

confidence: 99%

“…26 We have also used AIR to assess autoimmunity in Sjogrens disease and systemic lupus erythematosus. 16 Here, we have provided the first demonstration of the use of bacterial antigens on the AIR platform. The array is well-behaved analytically, with a quantitative dose−response behavior consistent with Langmuir surface binding.…”

Section: ■ Conclusionmentioning

confidence: 99%

“…All probe solutions contained trehalose as an additive to improve spot morphology. 16 Optimized print solutions used for the production of all arrays are shown in Table 1. An SX sciFLEXARRAYER (Scienion A.G.; Berlin, Germany) with a PDC70 capillary with type 4 coating was used to print a microarray of probe spots (Figure 2a) onto chips mounted onto adhesive strips inside a controlled humidity chamber at 72% ± 4% RH.…”

mentioning

confidence: 99%

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StaphAIR: A Label-Free Antigen Microarray Approach to Detecting Anti-Staphylococcus aureus Antibody Responses in Orthopedic Infections

Klose

Daiss

et al. 2021

Anal. Chem.

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Arrayed imaging reflectometry (AIR) is an optical biosensor platform for simple, multiplex measurement of antigenspecific antibody responses in patient blood samples. Here, we report the development of StaphAIR, an 8-plex Staphylococcus aureus antigen array on the AIR platform for profiling antigenspecific anti-S. aureus humoral immune responses. Initial validation experiments with mouse and humanized monoclonal antibodies against the S. aureus autolysin glucosaminidase (Gmd) domain, and subsequent testing with dilution series of pooled positive human serum confirmed analytically robust behavior of the array, with all antigens displaying Langmuir-type dose−response curves. Testing a cohort of 82 patients with S. aureus musculoskeletal infections (MSKI) and 30 healthy individuals enabled discrimination of individual patient responses to different S. aureus antigens, with statistical significance between osteomyelitis patients and controls obtained overall for four individual antigens (IsdA, IsdB, Gmd, and SCIN). Multivariate analyses of the antibody titers obtained from StaphAIR revealed its utility as a potential diagnostic tool for detecting S. aureus MSKI (area under the receiver operating characteristic curve (AUC) > 0.85). We conclude that StaphAIR has utility as a high-throughput immunoassay for studying and diagnosing osteomyelitis in patients.

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Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

StaphAIR: A Label-Free Antigen Microarray Approach to Detecting Anti-Staphylococcus aureus Antibody Responses in Orthopedic Infections

Klose

Daiss

et al. 2021

Anal. Chem.

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“…28 Binding of the analyte of interest to a capture spot results in a thickness change that perturbs the antireflection condition, resulting in light reflecting in proportion to the amount of material captured. The utility of AIR has been demonstrated in a broad range of applications, including detection and quantification of cytokines and other inflammatory biomarkers in human serum, 29 antibodies to human autoantigens, 30 and as a realtime method for monitoring protein-RNA binding. 31 Recently, we have also used the technique as a method for analyzing the human response to SARS-CoV-2 (COVID-19) infection.…”

Section: ■ Introductionmentioning

confidence: 99%

Label-Free, Multiplex Glycan Microarray Biosensor for Influenza Virus Detection

2021

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Newly emerging influenza viruses adapted from animal species pose significant pandemic threats to public health. An understanding of hemagglutinin (HA) receptor-binding specificity to host receptors is key to studying the adaptation of influenza viruses in humans. This information may be particularly useful for predicting the emergence of a pandemic outbreak. Therefore, high-throughput sensing technologies able to profile HA receptor binding can facilitate studies of influenza virus evolution and adaptation in humans. As a step toward this goal, we have prepared glycan-based receptor analogue microarrays on the Arrayed Imaging Reflectometry (AIR) platform. These arrays demonstrate label-free, multiplex detection and discrimination between human and avian influenza viruses. Microarrays consisting of glycan probes with 2,6 and 2,3 linkages were prepared. After first confirming their ability to capture lectins (carbohydrate-binding proteins) with known specificities, we observed that the arrays were able to discriminate between and quantify human pandemic influenza A/California/07/2009 (H1N1pdm) and avian A/Netherlands/1/2000 (H13N8) influenza viruses, respectively. As the method may be expanded to large numbers of glycans (>100) and virus subtypes (H1–H18), we anticipate it can be applied to systematically evaluate influenza virus adaptation in humans. In turn, this will facilitate global influenza surveillance and serve as a new tool enabling health organizations, governments, research institutes, and laboratories to react quickly in the face of a pandemic outbreak.

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Anti‐dsDNA, anti‐nucleosome, anti‐C1q, and anti‐histone antibodies as markers of active lupus nephritis and systemic lupus erythematosus disease activity

Shang

Ren

Sun

et al. 2021

Immunity Inflam & Disease

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Introduction Previous studies of anti‐dsDNA, nucleosome (Nucl), histone (His), and C1q antibodies have revealed their clinical value in systemic lupus erythematosus (SLE). However, the correlation between four autoantibodies and SLE activity, lupus nephritis (LN) remains controversial, and data are insufficient on longitudinal monitoring. This study aimed at evaluating the value of these autoantibodies in active LN, and their performance on cross‐sectional evaluating and longitudinal monitoring of SLE disease activity. Methods Serum levels of four autoantibodies in 114 SLE patients, 219 other autoimmune disease patients (OAD), and 59 healthy controls were assayed by a quantitative immunoassay. Sera of 38 inpatients were obtained again after treatment. Results We found that serum levels of four autoantibodies were significantly higher in SLE than OAD patients (p < 001), active LN than non‐renal SLE patients (p < .05), and higher in SLE patients with moderate and severe disease activity than mild disease activity (p < .01). Horizontally, serum level of each autoantibody was correlated with SLE disease activity index (SLEDAI) (p < .05), and correlation coefficient of anti‐dsDNA was the highest (r = .585). For longitudinal monitoring, the decreased levels of four autoantibodies were found following treatment (p < .001). Serum level variations of these antibodies were positively correlated with variations of SLEDAI (p < .05). The correlation coefficient of anti‐Nucl was the highest (r = .629). Although the levels of C3 and C4 increased after treatment, the change was not related to the change of SLEDAI (p > .05). Conclusions Anti‐C1q, anti‐dsDNA, anti‐Nucl, and anti‐His perform well in diagnosing active LN and monitoring SLE disease activity. They could be indicators of active LN and SLE disease activity.

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Label-free microarray-based detection of autoantibodies in human serum

Cited by 8 publications

References 24 publications

StaphAIR: A Label-Free Antigen Microarray Approach to Detecting Anti-Staphylococcus aureus Antibody Responses in Orthopedic Infections

StaphAIR: A Label-Free Antigen Microarray Approach to Detecting Anti-Staphylococcus aureus Antibody Responses in Orthopedic Infections

Label-Free, Multiplex Glycan Microarray Biosensor for Influenza Virus Detection

Anti‐dsDNA, anti‐nucleosome, anti‐C1q, and anti‐histone antibodies as markers of active lupus nephritis and systemic lupus erythematosus disease activity

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