Label free ultrasensitive detection of NS1 based on electrochemical aptasensor using polyethyleneimine aggregated AuNPs

Rashid, Sadia; Nawaz, Mian Hasnain; Marty, J.-L.; Hayat, Akhtar

doi:10.1016/j.microc.2020.105285

Cited by 21 publications

(10 citation statements)

References 41 publications

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“…The NS1, a nonstructural protein of Dengue [ 176 ] and Zika [ 177 ], the e antigen of hepatitis B (HBeAg) [ 178 , 179 ], the HIV reverse transcriptase, the HPV E7 protein [ 180 ], etc., were also acted as interesting targets for the detection of viruses.…”

Section: Viral Proteins and Their Detectionmentioning

confidence: 99%

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Aptamer-based biosensors for virus protein detection

Lou¹,

Liu²,

Shi³

et al. 2022

TrAC Trends in Analytical Chemistry

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Section: Viral Proteins and Their Detectionmentioning

confidence: 99%

“…Rashid et al [ 176 ] reported a label-free electrochemical aptasensor for detecting the NS1 protein of the Dengue virus. The principle of this aptasensor was the polyethyleneimine (PEI) and aptamer-assisted dispersion-aggregation of AuNPs, leading to changes in electrochemical signals.…”

Section: Viral Proteins and Their Detectionmentioning

confidence: 99%

Aptamer-based biosensors for virus protein detection

Lou¹,

Liu²,

Shi³

et al. 2022

TrAC Trends in Analytical Chemistry

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“…Although many POC devices have been developed so far, they show some limitations relating to time consumption, and even specificity and selectivity [ 96 , 97 ]. To overcome this situation, for the first time in the year 2020, Rashid et al have fabricated an electrochemical aptasensor for the detection of Dengue fever by targeting its NS1 protein [ 98 ]. NS1 is a non-structural protein which is considered as a specific biomarker for dengue fever [ 99 , 100 ].…”

Section: Label and Label-free Aptasensorsmentioning

confidence: 99%

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

et al. 2022

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Viral infections are becoming the foremost driver of morbidity, mortality and economic loss all around the world. Treatment for diseases associated to some deadly viruses are challenging tasks, due to lack of infrastructure, finance and availability of rapid, accurate and easy-to-use detection methods or devices. The emergence of biosensors has proven to be a success in the field of diagnosis to overcome the challenges associated with traditional methods. Furthermore, the incorporation of aptamers as bio-recognition elements in the design of biosensors has paved a way towards rapid, cost-effective, and specific detection devices which are insensitive to changes in the environment. In the last decade, aptamers have emerged to be suitable and efficient biorecognition elements for the detection of different kinds of analytes, such as metal ions, small and macro molecules, and even cells. The signal generation in the detection process depends on different parameters; one such parameter is whether the labelled molecule is incorporated or not for monitoring the sensing process. Based on the labelling, biosensors are classified as label or label-free; both have their significant advantages and disadvantages. Here, we have primarily reviewed the advantages for using aptamers in the transduction system of sensing devices. Furthermore, the labelled and label-free opto-electrochemical aptasensors for the detection of various kinds of viruses have been discussed. Moreover, numerous globally developed aptasensors for the sensing of different types of viruses have been illustrated and explained in tabulated form.

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“…An aptasensor based on the specific interaction between AuNPs, polyethyleneimine (PEI), aptamer NS1 and the NS1 protein, has been proposed for the detection of the NS1 protein of the dengue virus [ 107 ]. The well-dispersed AuNPs attached to the surface of the electrode repel the [Fe(CN) 6 ] −3/−4 anions from the interface, thus reducing the current signal as compared to the bare electrode.…”

Section: Electrochemical Aptasensors For Clinical Applicationmentioning

confidence: 99%

Electrochemical Aptasensors: Current Status and Future Perspectives

Abd-Ellatief

2021

Diagnostics

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This article reviews the progress of diversity of electrochemical aptasensor for target analytes detection. The immobilization strategies of aptamers on an electrode surface are addressed. The aptasensors are also introduced in compliance with the assay platforms. Many electrochemical aptasensors are nearly identical to conventional immunochemical approaches, sandwich and competition assays using electroactive signaling moieties. Others are “signal-on” and “sign-off” aptasensors credited to the target binding-induced conformational change of aptamers. Label-free aptasensors are also highlighted. Furthermore, the aptasensors applied for clinically important biomarkers are emphasized.

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Label free ultrasensitive detection of NS1 based on electrochemical aptasensor using polyethyleneimine aggregated AuNPs

Cited by 21 publications

References 41 publications

Aptamer-based biosensors for virus protein detection

Aptamer-based biosensors for virus protein detection

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

Electrochemical Aptasensors: Current Status and Future Perspectives

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