Labeling of steroids by activated tritium

Abstract

A5. a d d i t i o n r e a c t i o n , t h e f i n a l products from t h e u n s a t u r a t e d s t e r o i d s mays t i l l c o n t a i n s i g n i f i c a n t amounts of t h e l a b e l e d p a r e n t s . a c t i v i t i e s of t h e l a b e l e d by-products s t e r o i d s can approach 29 Ci/mM. S t e r o i d s with t h e 21-hydroxyl group are not t r i t i a t e d . Despite t h e

Recent Developments in Tritium Incorporation for Radiotracer Studies

“…tritium labeling of several groups of substrates, including steroids [34], benzodiazepines [35], polycyclic aromatic hydrocarbons and simple hydrocarbons [36]. Products have been characterized by GC or HPLC analyses, and by 3 H NMR spectroscopy.…”

Microwave‐Enhanced Radiochemistry

“…Wolf et al [28±30] improved the technique by circulating the tritium gas within the labeling system and successfully tritiated a number of tripeptides, amongst other compounds. Essentially the same system was used by Peng et al [31,32] to tritiate a number of steroids. The apparatus was relatively simple to construct and the MDA method was seen to be superior to the other radiation-induced methods of labeling, requiring a short reaction time and using a small amount of tritium gas.…”

Methods for the synthesis of tritium-labelled fatty acids and their derivatives, oxylipins and steroids

“…118 ± 122 The label can successfully be introduced into androstanes, pregnanes and some other steroids by a modified Wiltzbach procedure (see Ref. 123). Labelled steroids can also be prepared by biological methods, 120 hydrogenation and selective hydrogenation of the corresponding precursors 124 ± 127 in combination with other chemical methods (Table 22).…”