Laboratory biosafety measures involving SARS-CoV-2 and the classification as a Risk Group 3 biological agent

Kaufer, Alexa; Theis, Torsten; Lau, Katherine A.; Gray, Joanna; Rawlinson, William D.

doi:10.1016/j.pathol.2020.09.006

Cited by 64 publications

(50 citation statements)

References 16 publications

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“…In spite of the wide detection of SARS-CoV-2 RNA on surfaces by RT-qPCR and ddRT-qPCR, viable viruses were not yet confirmed. Most studies do not include viral infectivity assays because this requires SARS-CoV-2 propagation in cell culture that is only allowed in biosafety level 3 facilities (not widely available) ( Kaufer et al, 2020 ). Laboratory experiments with spiked samples have shown SARS-CoV-2 is stable in certain environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Surface contamination with SARS-CoV-2: A systematic review

Gonçalves

Silva

Reis

et al. 2021

Science of The Total Environment

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Little is known about contaminated surfaces as a route of transmission for SARS-CoV- 2 and a systematic review is missing and urgently needed to provide guidelines for future research studies. As such, the aim of the present study was to review the current scientific knowledge and to summarize the existing studies in which SARS-CoV-2 has been detected in inanimate surfaces. This systematic review includes studies since the emergence of SARS-CoV-2, available in PubMed/MEDLINE and Scopus. Duplicate publications were removed, and exclusion criteria was applied to eliminate unrelated studies, resulting in 37 eligible publications. The present study provides the first overview of SARS-CoV-2 detection in surfaces. The highest detection rates occurred in hospitals and healthcare facilities with COVID-19 patients. Contamination with SARS-CoV-2 on surfaces was detected in a wide range of facilities and surfaces. There is a lack of studies performing viability testing for SARS-CoV-2 recovered from surfaces, and consequently it is not yet possible to assess the potential for transmission via surfaces.

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Section: Discussionmentioning

confidence: 99%

Surface contamination with SARS-CoV-2: A systematic review

Gonçalves

Silva

Reis

et al. 2021

Science of The Total Environment

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“…Due to the serious health risks associated with working with SARS-CoV-2, it requires high levels of biosafety containment which is achievable only at selected laboratories [ 44 ]. Hence, the work presented in this paper was performed with FIPV, which was used as a surrogate/model virus for SARS-CoV-2.…”

Section: Discussionmentioning

confidence: 99%

Virucidal Efficacy of Blue LED and Far-UVC Light Disinfection against Feline Infectious Peritonitis Virus as a Model for SARS-CoV-2

Gardner

Ghosh

Dunowska

et al. 2021

Viruses

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Transmission of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs through respiratory droplets passed directly from person to person or indirectly through fomites, such as common use surfaces or objects. The aim of this study was to determine the virucidal efficacy of blue LED (405 nm) and far-UVC (222 nm) light in comparison to standard UVC (254 nm) irradiation for the inactivation of feline infectious peritonitis virus (FIPV) on different matrices as a model for SARS-CoV-2. Wet or dried FIPV on stainless steel, plastic, or paper discs, in the presence or absence of artificial saliva, were exposed to various wavelengths of light for different time periods (1–90 min). Dual activity of blue LED and far-UVC lights were virucidal for most wet and dried FIPV within 4 to 16 min on all matrices. Individual action of blue LED and far-UVC lights were virucidal for wet FIPV but required longer irradiation times (8–90 min) to reach a 4-log reduction. In comparison, LED (265 nm) and germicidal UVC (254 nm) were virucidal on almost all matrices for both wet and dried FIPV within 1 min exposure. UVC was more effective for the disinfection of surfaces as compared to blue LED and far-UVC individually or together. However, dual action of blue LED and far-UVC was virucidal. This combination of lights could be used as a safer alternative to traditional UVC.

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“…The combined effect of impaction, interception, and diffusion processes is that particles of all sizes whether higher or lower than 0.3 µ m are arrested in the HEPA filter ( First, 1998 ). The particles of size 0.3 µ m are called the most penetrating particles.…”

Section: Mechanism Of High Efficiency Particulate Air (Hepa) Filtrationmentioning

confidence: 99%

Selection and application of biological safety cabinets in diagnostic and research laboratories with special emphasis on COVID-19

Pawar

Khare

Keng

et al. 2021

Review of Scientific Instruments

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The ongoing coronavirus disease (COVID-19) pandemic is a global public health emergency. Adherence to biosafety practices is mandatory to protect the user as well as the environment, while handling infectious agents. A biological safety cabinet (BSC) is the most important equipment used in diagnostic and research laboratories in order to safeguard the product, the person, and the environment. The World Health Organization has emphasized the use of validated BSCs in order to ensure quality of the results. There are different classes of BSCs that are used in various work environments based on the need. It is imperative to use appropriate levels of biosafety and types of BSCs in laboratories based on the risk assessment of the pathogen used. During the development of COVID-19 laboratories and training of laboratory staff, we came across several queries about the functions and selection of BSCs and realized that the knowledge about the detailed information on selections and applications of BSCs is scanty. There are several guidelines regarding the biosafety aspects for diagnostic and research laboratories handling infectious pathogens from national and international agencies. However, there is no detailed information on the use of appropriate types of BSCs and their functions in the context of Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2). In view of this, the present paper describes in detail the selection and applications of BSCs, which could be useful for laboratories handling or planning to handle SARS-CoV-2 and suspected samples.

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Laboratory biosafety measures involving SARS-CoV-2 and the classification as a Risk Group 3 biological agent

Cited by 64 publications

References 16 publications

Surface contamination with SARS-CoV-2: A systematic review

Surface contamination with SARS-CoV-2: A systematic review

Virucidal Efficacy of Blue LED and Far-UVC Light Disinfection against Feline Infectious Peritonitis Virus as a Model for SARS-CoV-2

Selection and application of biological safety cabinets in diagnostic and research laboratories with special emphasis on COVID-19

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