Laboratory Diagnosis of Urinary Tract Infections: Guidelines, Challenges, and Innovations

Pezzlo, Marie

doi:10.1016/j.clinmicnews.2014.05.003

Cited by 32 publications

(29 citation statements)

References 29 publications

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“…These sticks can reveal the presence of leukocyte esterase or nitrite, which increases the probability of urinary infection. However, sensitivity of urinalysis is relatively low (especially low sensitivity at <10 5 cfu/ml) and have >15 % false positive rate [5].…”

Section: Introductionmentioning

confidence: 99%

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

et al. 2015

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Urinary tract infection (UTI) is a widespread infection and affects millions of people around the globe. The gold standard for identification of microorganisms causing infection is urine culture. However, current methods require at least 24 h for the results. In clinical settings, identification and discrimination of bacteria with less time-consuming and cheaper methods are highly desired. In recent years, the power of surface-enhanced Raman scattering (SERS) for fast identification of bacteria and biomolecules has been demonstrated. In this study, we show discrimination of urinary tract infection causative pathogens within 1 h of incubation using principal component analysis (PCA) of SERS spectra of seven different UTI causative bacterial species. In addition, we showed differentiation of them at their different growth phases. We also analyzed origins of bacterial SERS spectra and demonstrated the highly dynamic structure of the bacteria cell wall during their growth. Graphical Abstract Collection of bacteria from urine sample, and their discrimination using their SERS spectra and multivariate analysis.

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Section: Introductionmentioning

confidence: 99%

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

et al. 2015

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“…Urinary tract infections (UTIs) are among the most commonly occurring human infections [1,2]. It is estimated that approximately 50% of women will experience at least one UTI during their lifetime and that 25% will suffer recurrent infection [3].…”

Section: Introductionmentioning

confidence: 99%

“…Manuscript Pyelonephritis is distinct from cystitis and is commonly associated with fever (>38°C) and flank pain. The majority of cases of community-acquired cystitis are attributable to uropathogenic Escherichia coli (75-90%) and Staphylococcus saprophyticus (5-15%), with Klebsiella spp, Enterococcus spp., Streptococcus agalactiae and Proteus mirabilis accounting for most other cases [1,2,4,5]. Pseudomonas aeruginosa, Staphylococcus aureus and Candida spp.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of fosfomycin trometamol and nitrofurantoin in acute uncomplicated urinary tract infection

Parate¹

2016

Int J Med Res Rev

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Background: Acute urinary tract infection is stressful situation for women. Uncomplicated urinary tract infections are considered to be the most common bacterial infection in women. For treating these uncomplicated UTIs, susceptibility is of critical importance in selecting an appropriate antimicrobial agent. Material and Methods: A case control study carried out in department of obstetrics and gynecology in tertiary care teaching institute in north India. Total 100 female non pregnant patients aged 18 -65 years with acute uncomplicated UTI were taken in two groups each having 50 cases. The 50 patients of study group were given single 3 gram dose of fosfomycin trometalol orally, while the 50 patients of control group were given 100 mg tab of nitrofurantoin twice daily after food for 7 days, treated on OPD basis, Clinical and microbiological reassessment performed in 7-8 days after the start of treatment and long term follow up at one month from the start of the treatment were done. Results: Mean age of the patients in fosfomycin group was 31.6 years and in nitofurantoin group was 33.8 years. The most frequently isolated microorganism in both the groups were E coli 88% and 86 % respectively based on antibiotic susceptibility of all uropathogens, fosfomycin (91.8%) was the most active antibiotic followed by nitrofurantoin (91.2%) and norfloxacin. Bacterial eradication after 7 days follow up was obtained in 90 % in fosfomycin group as compared to 85% in control group. And long term follow up at one month gave eradication in 81 % in fosfomycin group as compared to 80 % in nitrofurantoin group. Conclusion: This study concludes that fosfomycin trometalol in a single 3-4 gram dose is as effective as 7 day regimen of nitrofurantoin for the treatment of uncomplicated lower urinary tract infection in women. So based on comparable microbiological cure and cost effectiveness and less adverse effects, we conclude that single dose fosfomycin trometalol is a good alternative in the treatment of women with acute uncomplicated UTI.

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“…[8][9][10][11][12][13][14] In particular, sensitivity and specificity have been targets of optimization and scrutiny in designing these sensors, with high attention to target type, native environment (e.g., the types of samples in which they are typically found), and testing conditions. Specifically, these tests have been enhanced to detect bioanalytes in a variety of conditions (e.g., wastewater, rinse water, soil, food, blood, tissue sample, and aerosol) despite the presence of interfering substances such as proteins, cells, lipids, and dust particles.…”

Section: Introductionmentioning

confidence: 99%

Biosensors for Monitoring Airborne Pathogens

Fronczek

Yoon

2015

SLAS Technology

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Airborne pathogens affect both humans and animals and are often highly and rapidly transmittable. Many problematic airborne pathogens, both viral (influenza A/H1N1, Rubella, and avian influenza/H5N1) and bacterial (Mycobacterium tuberculosis, Streptococcus pneumoniae, and Bacillus anthracis), have huge impacts on health care and agricultural applications, and can potentially be used as bioterrorism agents. Many different laboratory-based methods have been introduced and are currently being used. However, such detection is generally limited by sample collection, including nasal swabs and blood analysis. Direct identification from air (specifically, aerosol samples) would be ideal, but such detection has not been very successful due to the difficulty in sample collection and the extremely low pathogen concentration found in aerosol samples. In this review, we will discuss the portable biosensors and/or micro total analysis systems (µTAS) that can be used for monitoring such airborne pathogens, similar to smoke detectors. Current laboratory-based methods will be reviewed, and possible solutions to convert these lab-based methods into µTAS biosensors will be discussed.

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Laboratory Diagnosis of Urinary Tract Infections: Guidelines, Challenges, and Innovations

Cited by 32 publications

References 29 publications

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

A comparative study of fosfomycin trometamol and nitrofurantoin in acute uncomplicated urinary tract infection

Biosensors for Monitoring Airborne Pathogens

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