Laboratory Research in Homeopathy: Pro

Khuda‐Bukhsh, Anisur Rahman

doi:10.1177/1534735406294794

Cited by 47 publications

(27 citation statements)

References 61 publications

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“…The results of the present study as well as earlier evidence [27][28][29] support this contention. Admittedly, more work is necessary to arrive at a definite conclusion about the precise molecular mechanism(s) of action of homeopathic drugs, but that they are endowed with the capability to trigger gene expressions appears to be more convincing now.…”

Section: Discussionsupporting

confidence: 82%

Condurango 30C Induces Epigenetic Modification of Lung Cancer-specific Tumour Suppressor Genes via Demethylation

Khuda‐Bukhsh

Sikdar²

2015

Complement Med Res

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Background: DNA hypermethylation induces cancer progression involving CpG island of DNA and causes inactivation of tumour suppressor genes. In this study, DNA hypermethylation status of lung cancer and ability of ultra-highly diluted Condurango 30C to modulate DNA methylation were ascertained by analysis of lung cancer-specific tumour suppressor genes in respect to placebo. Materials and Methods: DNA methylation status, if any, was determined by PCR-SSCP analyses in lung cancer-specific tumour suppressor genes (p15, p16 and p53) using H460-NSCLC cell and BaP-induced lung cancer of rats. The ability of Condurango 30C to modulate DNA methylation, if any, was verified against placebo control in blinded manner. Results: Condurango 30C-treated DNA showed significant decrease in band intensity of p15 and p53 genes especially in methylated condition in vitro, at IC₅₀ dose (2.43µl/100µl). SSCP analysis of p15 and p53 genes in Condurango 30C-treated DNA also suggests that Condurango 30C can decrease methylation, in vitro. Inhibition of p15 hypermethylation was observed in post-cancer treatment of rats with Condurango 30C. SSCP results gave a better indication of differences in band position of p15 and p53 in Condurango 30C-treated lung samples. Conclusion: Condurango 30C could trigger epigenetic modification in lung cancer via modulation of DNA hypermethylation.

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Section: Discussionsupporting

confidence: 82%

Condurango 30C Induces Epigenetic Modification of Lung Cancer-specific Tumour Suppressor Genes via Demethylation

Khuda‐Bukhsh

Sikdar²

2015

Complement Med Res

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“…How the ultra low doses of the remedy, which are not theoretically expected to possess even a single molecule of the original drug substance, could bring about multiple changes in both cytogenetical and enzyme biomarkers, is rather unclear at the present state of our knowledge. Incidentally, Khuda‐Bukhsh (Khuda‐Bukhsh, 1997, 2003, 2006; Pathak et al., 2006) proposed a hypothesis that one mechanism through which the potentized homeopathic drugs act could be through regulation of expression of certain relevant genes, by acting as suitable ‘molecular switch’ with the ability to trigger a cascade of gene actions, as all the cytogenetical and biochemical markers tested are generally under the strict control of a specific genetic regulatory mechanism.…”

Section: Discussionmentioning

confidence: 99%

A Potentized Homeopathic Drug, Arsenicum Album 200, Can Ameliorate Genotoxicity Induced by Repeated Injections of Arsenic Trioxide in Mice

Banerjee

Biswas

Belon

et al. 2007

Journal of Veterinary Medicine Series A

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Summary Groundwater arsenic contamination has become a menacing global problem. No drug is available until now to combat chronic arsenic poisoning. To examine if a potentized homeopathic remedy, Arsenicum Album‐200, can effectively combat chronic arsenic toxicity induced by repeated injections of Arsenic trioxide in mice, the following experimental design was adopted. Mice (Mus musculus) were injected subcutaneously with 0.016% arsenic trioxide at the rate of 1 ml/100 g body weight, at an interval of 7 days until they were killed at day 30, 60, 90 or 120 and were divided into three groups: (i) one receiving a daily dose of Arsenicum Album‐200 through oral administration, (ii) one receiving the same dose of diluted succussed alcohol (Alcohol‐200) and (iii) another receiving neither drug, nor succussed alcohol. The remedy or the placebo, as the case may be, was fed from the next day onwards after injection until the day before the next injection, and the cycle was repeated until the mice were killed. Two other control groups were also maintained: one receiving only normal diet, and the other receiving normal diet and succussed alcohol. Several toxicity assays, such as cytogenetical (chromosome aberrations, micronuclei, mitotic index, sperm head anomaly) and biochemical (acid and alkaline phosphatases, lipid peroxidation), were periodically made. Compared with controls, the drug fed mice showed reduced toxicity at statistically significant levels in respect of all the parameters studied, thereby indicating protective potentials of the homeopathic drug against chronic arsenic poisoning.

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“…Incidentally, KhudaBukhsh [39][40][41] has proposed a working hypothesis that one mechanism through which the potentized homeopathic drugs act could be regulation of expression of certain relevant genes. This explanation seems to be plausible because all biomarkers tested are regulated by specific genetic regulatory mechanisms, and without involvement of these regulatory mechanisms, such positive results could not have been achieved.…”

Section: Discussionmentioning

confidence: 99%

Comparative Efficacy of Two Microdoses of a Potentized Homeopathic Drug, Arsenicum Album, to Ameliorate Toxicity Induced by Repeated Sublethal Injections of Arsenic Trioxide in Mice

Banerjee

Bhattacharyya²,

Pathak³

et al. 2008

Pathobiology

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Objectives: To evaluate the efficacy of 2 potentized homeopathic remedies of Arsenicum Album (Ars Alb) – 6C and 30C – in combating chronic arsenic toxicity induced by repeated sublethal injections in mice (Mus musculus). Methods: Mice were randomized and divided into sets: (1) normal (control 1); (2) normal + succussed alcohol (control 2); (3) As₂O₃ (0.016%) injected at 1 ml/100 g body weight every 7 days (treated); (4) As₂O₃ injected + succussed alcohol (positive control); (5) As₂O₃ injected + Ars Alb 6C (drug-fed); (6) As₂O₃ injected + Ars Alb 30C (drug-fed). Cytogenetical endpoints like chromosome aberrations, micronuclei, mitotic index, sperm head abnormality and biochemical protocols like acid and alkaline phosphatases, aspartate and alanine aminotransferases, reduced glutathione, lipid peroxidation, catalase and succinate dehydrogenase were studied at 30, 60, 90 and 120 days. Results: Compared to controls, chromosome aberrations, micronuclei, sperm head abnormality frequencies and activities of acid and alkaline phosphatases, aspartate and alanine aminotransferases and lipid peroxidation were reduced in both drug-fed series, while mitotic index and activities of glutathione, catalase and succinate dehydrogenase were increased. Ars Alb 30C showed marginally better efficacy than Ars Alb 6C. Conclusion: Both remedies indicated potentials of use against arsenic intoxication.

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Laboratory Research in Homeopathy: Pro

Cited by 47 publications

References 61 publications

Condurango 30C Induces Epigenetic Modification of Lung Cancer-specific Tumour Suppressor Genes via Demethylation

Condurango 30C Induces Epigenetic Modification of Lung Cancer-specific Tumour Suppressor Genes via Demethylation

A Potentized Homeopathic Drug, Arsenicum Album 200, Can Ameliorate Genotoxicity Induced by Repeated Injections of Arsenic Trioxide in Mice

Comparative Efficacy of Two Microdoses of a Potentized Homeopathic Drug, Arsenicum Album, to Ameliorate Toxicity Induced by Repeated Sublethal Injections of Arsenic Trioxide in Mice

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