2015
DOI: 10.1016/j.procbio.2015.05.003
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Laccase mediated oxidation of industrial lignins: Is oxygen limiting?

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Cited by 52 publications
(51 citation statements)
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“…Commercial laccase from M. thermophila showed the ability to oxidize and polymerize Kraft lignin (KL). The extension of this polymerization depended on several parameters such as lignin origin, pH, temperature, phenolic mediators or reaction time [11,12,22,23]. The aim of this study was to test the capability of the enzymatic treatment of KL in the manufacturing of MDF with no synthetic resins.…”
Section: Resultsmentioning
confidence: 99%
“…Commercial laccase from M. thermophila showed the ability to oxidize and polymerize Kraft lignin (KL). The extension of this polymerization depended on several parameters such as lignin origin, pH, temperature, phenolic mediators or reaction time [11,12,22,23]. The aim of this study was to test the capability of the enzymatic treatment of KL in the manufacturing of MDF with no synthetic resins.…”
Section: Resultsmentioning
confidence: 99%
“…20−22 Fluorescence Intensity Measurements. Fluorescence intensity was measured as already described in Ortner et al 15 One hundred microliters of lignosulfonate taken at the times described above were incubated with 120 μL of a methoxyethanol:water (2:1 v/v) mixture, and the absorbance was measured at an excitation of 355 nm and an emission of 400 nm using a spectrophotometer (Infinite M200, Tecan, Switzerland).…”
Section: ■ Materials and Methodsmentioning
confidence: 99%
“…First, 5 mL of a 10% w·v −1 solution of Kraft lignin (KL) or lignosulfonate (LS) were prepared in double distilled water (ddH 2 O) and the pH was adjusted to 7, which is the optimal pH for the used laccase [ 8 ]. In addition, 50 or 100 mg of immobilized preparation were then added to the reaction solution and the polymerization process was conducted for 24 h at 21 °C and 400 rpm.…”
Section: Methodsmentioning
confidence: 99%
“…It is well-known that laccase-mediated polymerization may cause condensation reactions between the phenoxy radicals that are formed resulting in new C–C, aryl-aryl, ether, or aryl-alkyl cross-linkages [5]. Ortner et al studied the oxidation of various industrial lignins (Organosolv, Indulin and lignosulfonates) aqueous solutions and the potential of external oxygen supply was demonstrated, which was also strengthened by recent findings [8,9]. Additionally, polymerization of various lignins (Organosolv hardwood lignin, soda wheat straw lignin, alkali pretreated wheat straw lignin, kraft softwood, etc.)…”
Section: Introductionmentioning
confidence: 92%