Lack of cardioprotection by single-dose magnesium prophylaxis on isoprenaline-induced myocardial infarction in adult Wistar rats

Abstract

SummaryAimMagnesium (Mg2+) is effective in treating cardiovascular disorders such as arrhythmias and pre-eclampsia, but its role during myocardial infarction (MI) remains uncertain. In this study, we investigated the effects of Mg2+ pre-treatment on isoprenaline (ISO)-induced MI in vivo.MethodsRats divided into four groups were each pre-treated with either MgSO4 (270 mg/kg intraperitoneally) or an equivalent volume of physiological saline, prior to the ISO (67 mg/kg subcutaneously) or saline treatments. One day post-treatment, the electrocardiogram and left ventricular blood pressures were recorded. Infarcts were determined using 2,3,5-triphenyltetrazolium chloride staining, and serum markers of lipid peroxidation were measured with spectrophotometric assays.ResultsMg2+ pre-treatment neither altered the ISO-induced infarct size compared with ISO treatment alone (p > 0.05), nor reversed the low-voltage electrocardiogram or the prominent Q waves induced by ISO, despite a trend to decreased Q waves. Similarly, Mg2+ did not prevent the ISO-induced decrease in peak left ventricular blood pressure or the decrease in minimal rate of pressure change. Mg2+ did not reverse the ISO-induced gain in heart weight or loss of body weight. Neither ISO nor Mg2+ altered the concentrations of lipid peroxidation markers 24 hours post MI induction.ConclusionAlthough Mg2+ had no detrimental effects on electrical or haemodynamic activity in ISO-induced MI, the lack of infarct prevention may detract from its utility in MI therapy.

(E)-N′-(1-(7-Hydroxy-2-Oxo-2H-Chromen-3-Yl) Ethylidene) Benzohydrazide, a Novel Synthesized Coumarin, Ameliorates Isoproterenol-Induced Myocardial Infarction in Rats through Attenuating Oxidative Stress, Inflammation, and Apoptosis

“…Oxidative stress has been considered a conjoint pathological mechanism, and it contributes to initiation and progression of various cardiovascular dysfunctions, such as myocardial infarction (MI) [30,31]. Moreover, many researchers have suggested that oxidative stress induces amplification of the inflammatory response and apoptosis leading to the initiation of ischemic lesion [32,33].…”

A novel synthetised sulphonylhydrazone coumarin (E)‐4‐methyl‐N′‐(1‐(3‐oxo‐3H‐benzo[f]chromen‐2‐ yl)ethylidene)benzenesulphonohydrazide protect against isoproterenol‐induced myocardial infarction in rats by attenuating oxidative damage, biological changes and electrocardiogram

“…When used in excessive doses, isoproterenol exerts deleterious effects on the heart of myocardial infarction model rats, leading to many morphological, pathophysiological, and metabolic aberrations comparable to those taking place in human MI. [4][5][6][7] Since MI remains a public health problem resulting in morbidities and mortalities at higher rates, 8 several studies have been focused, over the last few years, on the development of new synthetic compounds with promising pharmacological activity for prevention and treatment of MI or cardiovascular diseases. [9][10][11] Coumarins, acting as antagonists to vitamin K, are widespread natural chemicals found in a variety of plant sources that represent the most promising cardioprotective compounds playing an important role against thromboembolic diseases.…”

“…Since MI remains a public health problem resulting in morbidities and mortalities at higher rates, 8 several studies have been focused, over the last few years, on the development of new synthetic compounds with promising pharmacological activity for prevention and treatment of MI or cardiovascular diseases 9–11 . Coumarins, acting as antagonists to vitamin K, are widespread natural chemicals found in a variety of plant sources that represent the most promising cardioprotective compounds playing an important role against thromboembolic diseases 12 .…”

Integration of Metabolomics With Pharmacodynamics to Elucidate the Anti-myocardial Ischemia Effects of Combination of Notoginseng Total Saponins and Safflower Total Flavonoids

“…MI is characterized by an acute condition of ischemic necrosis of heart muscle that occurs as a result of the interruption in the supply of myocardial oxygen and nutrients ( Zhou et al, 2008 ). Overstimulation of isoproterenol (ISO), a synthetic β-adrenergic receptor agonist was reported to induce MI through severe cardiac stress, including the highly cytotoxic free radicals producing, intracellular Ca 2+ overload, and apoptosis ( Garson et al, 2015 ). The experimental model of ISO-induced MI offered a reliable technique to investigate the efficacy of various potentially cardioprotective agents, which has been frequently documented in previous studies ( Fathiazad et al, 2012 ).…”