Lack of chitin synthase genes impacts capsular architecture and cellular physiology in Cryptococcus neoformans

Rodrigues, Jéssica; Ramos, Caroline L.; Frasés, Susana; Godinho, Rodrigo M. da C.; Fonseca, Fernanda L.; Rodrigues, Márcio L.

doi:10.1016/j.tcsw.2018.05.002

Cited by 20 publications

(15 citation statements)

References 38 publications

(86 reference statements)

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“…Cell wall thickness did not interfere with vesicle passage since C. albicans mutants with altered cell wall dimensions and composition were similar in both their in vitro susceptibility to liposomal amphotericin B and the ability of liposomes to penetrate the cell wall (Walker et al, ). This observation is in agreement with the fact that in C. neoformans chitin synthase mutants EV production and cell wall porosity had no apparent correlation (Rodrigues et al, ).…”

Section: Going Back Inside: Uptake Of Evs By Fungal Cellssupporting

confidence: 89%

A two‐way road: novel roles for fungal extracellular vesicles

Rodrigues

Casadevall

2018

Molecular Microbiology

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The biological functions of fungal extracellular vesicles (EVs) or exosomes have been mostly determined on the basis of the assumption that they are vehicles of trans-cell wall transport and molecular export. The possibility that fungal cells can bind to and internalize EVs remained largely unaddressed. Recent studies, however, demonstrated that fungal cells can internalize host-derived and/or fungal EVs through processes that profoundly modify their regular physiology. To illustrate this novel view, we discuss (i) the uptake of plant EVs by phytopathogenic fungi culminating in growth defects and virulence attenuation, (ii) the influence of EV internalization in prion transmission and biofilm formation in yeast cells, and (iii) the EV-mediated transfer of virulence in isolates of Cryptococcus gattii. These recent observations indicate that the functions exerted by EVs in fungal cells result from previously unknown mechanisms of bidirectional transport, opening new venues for the investigation of how EVs impact fungal physiology.

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Section: Going Back Inside: Uptake Of Evs By Fungal Cellssupporting

confidence: 89%

A two‐way road: novel roles for fungal extracellular vesicles

Rodrigues

Casadevall

2018

Molecular Microbiology

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“…For instance, a C. neoformans mutant lacking a putative G 1 /S cyclin gene had increased production of EVs (Garcia-Rodas et al, 2014 ). Chitin synthase genes (CHS) were also suggested to play a role in vesicular release, as inferred from the observation that the deletion of chitin synthases genes in C. neoformans resulted in a significantly reduced release of EVs (Rodrigues et al, 2018 ). Recently, it was also shown that S. cerevisiae cytoplasmic organelles, called intracellular vesicle clusters (IVCs), serve as sites for the synthesis and selection of EV-associated proteins tagged for secretion (Winters et al, 2020 ).…”

Section: Biogenesis Selection Of Cargo and Release Of Evs In Fungimentioning

confidence: 99%

Extracellular Vesicles in Fungi: Past, Present, and Future Perspectives

Rizzo

Rodrigues

Janbon

2020

Front. Cell. Infect. Microbiol.

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109

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Extracellular vesicles (EVs) have garnered much interest in the cell biology and biomedical research fields. Many studies have reported the existence of EVs in all types of living cells, including in fifteen different fungal genera. EVs play diverse biological roles, from the regulation of physiological events and response to specific environmental conditions to the mediation of highly complex interkingdom communications. This review will provide a historical perspective on EVs produced by fungi and an overview of the recent discoveries in the field. We will also review the current knowledge about EV biogenesis and cargo, their role in cell-to-cell interactions, and methods of EV analysis. Finally, we will discuss the perspectives of EVs as vehicles for the delivery of biologically active molecules.

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“…For the SEM, the treated H99 cells were washed with PBS and fixed by 2.5% glutaraldehyde. The specimens for SEM were prepared according to the standard sample preparation method as described ( 63 ). The gold-coated samples were scanned by the SEM (Hitachi SU8010).…”

Section: Methodsmentioning

confidence: 99%