Lactatdehydrogenase (LDH) prior and post implantation of ATS® heart valves

Matschke, Klaus; Schade, Ina; Kappert, Utz; Tugtekin, Sems Malte; Schneider, Jens; Jung, F.; Knaut, Michael

doi:10.1016/j.ijcard.2004.12.029

Cited by 4 publications

(3 citation statements)

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“…The effect observed in this study may be of major clinical significance considering that LDH is measured e.g. during heart surgery and serves as an important prognostic factor in cardiac interventions [1,10,15,18,20]. Furthermore, LDH also has high relevance as a marker for ischemic processes and cell necrosis, both in vitro [9,17,24] and in vivo [2,4,19,23].…”

Section: Discussionmentioning

confidence: 59%

“…This leads to the release of elevated quantities of intracellular components, such as LDH and other substances, into the cell culture medium or into the circulation system. Thus the detection of LDH in cell culture media [22,26], in the animal model [8], and in the blood plasma of patients [14,20] is regarded as an indicator of impaired cell membrane integrity [3,25].…”

Section: Introductionmentioning

confidence: 99%

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Reduced diagnostic value of lactate dehydrogenase (LDH) in the presence of radiographic contrast media

Franke

Fuhrmann

Mrowietz³

et al. 2010

Clinical Hemorheology and Microcirculation

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Isoforms of the enzyme lactate dehydrogenase (LDH) were found in almost all cells of the organism and an elevated activity of LDH in the circulation is thought to be a clear indicator of elevated cell destruction coinciding with an increased release of components from the cellular cytoplasm, e.g. LDH. Here, we report on an in-vitro examination to test whether radiographic contrast media (RCM) could induce cell destruction followed by an increase in LDH release. The RCM were tested in non-flow cultures of human umbilical venous endothelial cells (HUVEC) of the fourth passage seeded on extracellular matrix and the results were compared to those from control cultures not exposed to contrast media.The examination revealed that the addition of contrast media to the cell culture media supplemented with pooled human serum (HSP) as source of exogenous LDH was followed by a strong decrease in LDH activity both in the absence and presence of HUVEC. Within 1.5 min after the addition of contrast media to the culture medium supplemented with HSP (30% vol of the culture medium were replaced by either of two contrast media, Iodixanol or Iopromide) the LDH activity decreased about 80% compared to the initial values. In contrast, the LDH activity did not change in cell culture media not supplemented with RCM.The partial replacement of HSP supplemented cell culture medium by RCM will cause a dilution of cell culture medium constituents. The decrease of LDH activity, however, was much stronger than the decrease thought to be attributable to the effects of dilution of cell culture medium, so that the role of dilution seems to be a minor one in this case. It has to be assumed that the RCM could interact with the LDH available in the culture medium as well as with the substrates delivered with the measurement system for the assessment of LDH activity, so that both, the amount of LDH and the activities of enzymes involved might be influenced.In the presence of HUVEC a similar effect was observed. Here, a little less strong decrease of LDH activity occurred compared to the decrease in cell culture medium without HUVEC. This was unexpected because a considerable amount of HUVEC were detached after the addition of contrast media and many of these cells were damaged seriously so that a significant amount of endogenous LDH should have been released.These unexpected results make it necessary to re-evaluate those past time examinations focussed on cell damage/destruction in the presence of contrast media, where the measurement of LDH activity was used as indicator or cell vitality and where cell decease rates were correlated to questionable toxic influences. According to the results of the examination reported here it is difficult to uphold the interpretation of recently published findings that contrast media almost exclusively induce cellular apoptosis and not necrosis.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Reduced diagnostic value of lactate dehydrogenase (LDH) in the presence of radiographic contrast media

Franke

Fuhrmann

Mrowietz³

et al. 2010

Clinical Hemorheology and Microcirculation

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“…It is accepted that determination of LDH and CK activity provides one of the biochemical indexes for the evaluation and diagnosis of heart disease, due to the level of LDH in serum reflecting injury in the permeability of the cardiomyocytes, and the activity level of CK being directly related to the consumption and supply of myocardial oxygen and energy, muscle contraction, and mitochondrial function ( Agress, 1965 ; Matschke et al, 2005 ; Ingwall, 2009 ; Zervou et al, 2016 ; Bak and Schousboe, 2017 ; Klein et al, 2020 ). Furthermore, the mitochondrial membrane potential and the intensity of calcium fluorescence, which were testing indexes in the present study, play an essential role in the mitochondrial function of myocardial tissue homeostasis ( Skarka and Ostadal, 2002 ; Dibb et al, 2007 ; Kadenbach et al, 2011 ; Davlouros et al, 2016 ; Zorova et al, 2018 ; Schartner et al, 2019 ; Lai and Qiu, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Potential cardiotoxicity induced by Euodiae Fructus: In vivo and in vitro experiments and untargeted metabolomics research

Zhang

Lü²,

Ren³

et al. 2022

Front. Pharmacol.

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Background: Euodiae Fructus, a well-known herbal medicine, is widely used in Asia and has also gained in popularity in Western countries over the last decades. It has known side effects, which have been observed in clinical settings, but few studies have reported on its cardiotoxicity.Methods: In the present study, experiments using techniques of untargeted metabolomics clarify the hazardous effects of Euodiae Fructus on cardiac function and metabolism in rats in situations of overdosage and unsuitable syndrome differentiation. In vitro assays are conducted to observe the toxic effects of evodiamine and rutaecarpine, two main chemical constituents of Euodiae Fructus, in H9c2 and neonatal rat cardiomyocytes (NRCMs), with their signaling mechanisms analyzed accordingly.Results: The cardiac cytotoxicity of evodiamine and rutaecarpine in in vivo experiments is associated with remarkable alterations in lactate dehydrogenase, creatine kinase, and mitochondrial membrane potential; also with increased intensity of calcium fluorescence, decreased protein expression of the cGMP-PKG pathway in H9c2 cells, and frequency of spontaneous beat in NRCMs. Additionally, the results in rats with Yin deficiency receiving a high-dosage of Euodiae Fructus suggest obvious cardiac physiological dysfunction, abnormal electrocardiogram, pathological injuries, and decreased expression of PKG protein. At the level of endogenous metabolites, the cardiac side effects of overdose and irrational usage of Euodiae Fructus relate to 34 differential metabolites and 10 metabolic pathways involving among others, the purine metabolism, the glycerophospholipid metabolism, the glycerolipid metabolism, and the sphingolipid metabolism.Conclusion: These findings shed new light on the cardiotoxicity induced by Euodiae Fructus, which might be associated with overdose and unsuitable syndrome differentiation, that comes from modulating the cGMP-PKG pathway and disturbing the metabolic pathways of purine, lipid, and amino acid. Continuing research is needed to ensure pharmacovigilance for the safe administration of Chinese herbs in the future.

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Heme oxygenase derived carbon monoxide and iron mediated plasmatic hypercoagulability in a patient with calcific mitral valve disease

Thompson

Nielsen

Castro

et al. 2014

J Thromb Thrombolysis

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We present a case of a patient with calcific mitral valve stenosis and plasmatic hypercoagulability. Using thrombelastography, the patient was determined to have an abnormally large velocity of plasma thrombus growth and strength with reduced vulnerability to lysis. Critically, increased carboxyhemoglobin concentration (2.4 %) was present, likely secondary to hemolysis from mitral stenosis and engagement of systemic heme oxygenase. It was determined that the patient's plasmatic hypercoagulability was in part due to carboxyhemefibrinogen formation and iron-enhancement of coagulation via two thrombelastographic methods. In conclusion, future investigation of the involvement of both carbon monoxide and iron mediated hypercoagulability in the setting of stenotic valve disease is warranted.

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Lactatdehydrogenase (LDH) prior and post implantation of ATS® heart valves

Cited by 4 publications

References 3 publications

Reduced diagnostic value of lactate dehydrogenase (LDH) in the presence of radiographic contrast media

Reduced diagnostic value of lactate dehydrogenase (LDH) in the presence of radiographic contrast media

Potential cardiotoxicity induced by Euodiae Fructus: In vivo and in vitro experiments and untargeted metabolomics research

Heme oxygenase derived carbon monoxide and iron mediated plasmatic hypercoagulability in a patient with calcific mitral valve disease

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