Lactate as clinical tumour biomarker: Optimization of lactate detection and quantification in MR spectroscopic imaging of glioblastomas

Bisdas, Sotirios; Schäfer, R; Kolb, Rupert; Bender, Benjamín; Klose, Uwe

doi:10.1016/j.ejrad.2020.109171

Cited by 13 publications

(11 citation statements)

References 15 publications

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“…Lactate promoted sEV release by B16-F10 and Hepa1-6 cells in a dose-dependent manner in vitro ( Figure S1A ), and treatment with 10 mM lactate did not affect the viability of either cell line ( Figure S1B ). A higher lactate concentration of 10 mM is reported in tumor tissues ( 22 , 23 ), so 10 mM lactate was used in subsequent in vitro experiments. We further confirmed lactate promoted TEV production by assessing the total protein amount of sEVs, the particle concentration of sEVs and the levels of sEV proteins from equal numbers of cells ( Figures 1A-C ).…”

Section: Resultsmentioning

confidence: 99%

Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis

Man

Zhu²,

Ren³

et al. 2022

Front. Oncol.

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Lactate and tumor cell-derived extracellular vesicles (TEVs) both contribute to tumor progression. However, it is still unclear whether lactate can accelerate tumor development by directly promoting TEV production. Here, we show that lactate decreases intracellular cAMP levels and subsequent PKA activation via GPR81, which inhibits the PKA-induced ubiquitination of HIF-1α that causes degradation. Then, the HIF-1α-mediated transcription of Rab27a is enhanced, leading to increased TEV release. In this way, lactate promotes lung metastasis by murine melanoma. In addition, we show that serum lactate levels are positively correlated with serum EV levels and Rab27a and HIF-1α protein levels in the tumor tissues of lung cancer patients. Thus, our results reveal a novel mechanism underlying lactate-mediated tumor progression induced by TEVs and provide new strategies for tumor therapy.

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Section: Resultsmentioning

confidence: 99%

Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis

Man

Zhu²,

Ren³

et al. 2022

Front. Oncol.

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“…For this reason, ex vivo 1 H-NMR spectra from intact tissue samples usually exhibit particularly intense lactate resonances, which are to a great extent artifacts introduced by anaerobic glycolysis induced by a lack of oxygen, the latter being the result of ischemia. This is true notwithstanding that many tumors typically produce large amounts of lactate in vivo [21,22]. Tumor-generated lactate would also be detectable in excised tissue and in spheroids, but undetectable in cells harvested and/or extracted from monolayer cultures because lactate produced by tumor cells is often efficiently exported into the extracellular space (see [23] for 1 H-NMR spectroscopy of extra vs. intracellular metabolism).…”

Section: In Vitro Vs In Vivo Nmr Spectroscopic Analysismentioning

confidence: 99%

Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls

Lutz

Bernard

2022

Molecules

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This is a broad overview and critical review of a particular group of closely related ex vivo and in vitro metabolic NMR spectroscopic methods. The scope of interest comprises studies of cultured cells and excised tissue, either intact or after physicochemical extraction of metabolites. Our detailed discussion includes pitfalls that have led to erroneous statements in the published literature, some of which may cause serious problems in metabolic and biological interpretation of results. To cover a wide range of work from relevant research areas, we consider not only the most recent achievements in the field, but also techniques that proved to be valid and successful in the past, although they may not have generated a very significant number of papers more recently. Thus, this comparative review also aims at providing background information useful for judiciously choosing between the metabolic ex vivo/in vitro NMR methods presented. Finally, the methods of interest are discussed in the context of, and in relation to, other metabolic analysis protocols such as HR-MAS and cell perfusion NMR, as well as the mass spectrometry approach.

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“…Tissue lactate can be detected by magnetic resonance spectroscopy. Recently, steps have been taken to make these diagnostic processes more reliable [ 23 ]. Detection of cerebral lactate with magnetic resonance spectroscopy has been repeatedly associated with poorer survival [ 24 ].…”

Section: Lactatementioning

confidence: 99%

Hyperlactatemia and other perioperative metabolic disturbances in neuroanesthesia

Klimek

Smalen

Janssen

2022

Current Opinion in Anaesthesiology

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Purpose of reviewThe concept of 'brain-body cross-talking' has gained growing interest in the last years. The understanding of the metabolic disturbances (e.g., hypernatraemia/hyponatraemia and hyperlactatemia) in neurosurgical patients has improved during the last years. Recent findingsThe impact of elevated lactate without acidosis in neurosurgical patients remains controversial. The pathophysiology of inappropriate secretion of antidiuretic hormone (SIADH) has become clearer, whereas the diagnosis of cerebral salt wasting should be used more carefully.

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Lactate as clinical tumour biomarker: Optimization of lactate detection and quantification in MR spectroscopic imaging of glioblastomas

Cited by 13 publications

References 15 publications

Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis

Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis

Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls

Hyperlactatemia and other perioperative metabolic disturbances in neuroanesthesia

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