Lactate dehydrogenase isoenzyme patterns of human lymphatic and myeloid leukemic leukocytes

Hule, V

doi:10.1016/0009-8981(67)90208-2

Cited by 8 publications

(5 citation statements)

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“…Recent studies (Dioguardi, Agostoni, Fiorelli, and Lomanto, 1963;Bottomley, Locke, and Ingram, 1966;Hule, 1967) indicate that polymorphonuclear cells and lymphocytes have different LDH isoenzyme patterns. One may thus expect to find LDH shifts in parallel with the type of the cellular material.…”

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Lactate dehydrogenase in synovial fluid diagnostic evaluation of total activity and isoenzyme patterns.

Em¹,

Wieme²

1968

Annals of the Rheumatic Diseases

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The work of Ropes and Bauer (1953) has focused attention on the analysis of synovial fluid in the differential diagnosis of joint effusion. White blood cell total and differential counts, glucose assay, and mucin precipitation tests have thus all been applied to the distinction of non-inflammatory, mild inflammatory, severe inflammatory, and infectious effusions. These analyses do not, however, indicate the true origin of the effusion, and we have carried out this investigation to find out whether differential assay of lactate dehydrogenase (LDH) isoenzymes could yield more specific information.It is known that the percentage of polymorphonuclear cells (PMN) and of lymphocytes is different in non-inflammatory, inflammatory, and infectious effusions. Recent studies (Dioguardi, Agostoni, Fiorelli, and Lomanto, 1963;Bottomley, Locke, and Ingram, 1966;Hule, 1967) indicate that polymorphonuclear cells and lymphocytes have different LDH isoenzyme patterns. One may thus expect to find LDH shifts in parallel with the type of the cellular material. It could also be of interest to look for any enzyme contribution originating in the lining walls of the synovia.Vesell, Osterland, Bearn, and Kunkel (1962) and especially Cohen (1964), who studied the LDH activity of synovial fluid in rheumatic disease states, reported that total synovial fluid LDH was raised in patients with inflammatory articular disease, and found marked rises of LDH5 in total synovial fluids of rheumatoid, gouty, and infected joints. They only found a minimal increase of LDH5 in fluids from cases of degenerative joint disease. Although these observations suggest that this type of analysis will be of little diagnostic value, one must remember that Vesell and others (1962) studied only a limited number of cases, including several of ill-defined aetiology. Cohen (1964) used LDH isoenzyme differentiation only as a complement to other enzyme studies; he confined himself to a visual interpretation of the LDH patterns in a relatively small number of cases.We therefore felt that interesting results might be obtained by applying a quantitative technique of LDH differentiation to a large number of cases of well-established aetiology.We have also determined LDH isoenzyme patterns separately on cell-free fluid and on cellular material after ultrasonic disintegration. MethodsSynovial fluids were aspirated with sterile precautions from different joints (knee, elbow, hip, ankle) without using local anesthetics. In supplement to the analyses mentioned, the fluids were also submitted to bacteriological culture and to differential count of the white blood cells.Before the determination of total LDH activity and analysis of the LDH patterns, a constant amount of the synovial fluid (10 ml.) was centrifuged at 50,000 G. and 100 C. temperature for 30 min. In this way a clear cell-free supernatant and a cellular sediment were obtained. The supernatant was used as such. The sediment was washed three times with saline and finally resuspended in exactly 5 ml. saline. This suspensio...

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Lactate dehydrogenase in synovial fluid diagnostic evaluation of total activity and isoenzyme patterns.

Em¹,

Wieme²

1968

Annals of the Rheumatic Diseases

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“…Farriaux et al 1967). In leukaemia the shift appears to depend on the type of leukaemia (Hule 1967, Rabinowitz & Dietz 1967.…”

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confidence: 97%

Lactate Dehydrogenase Isoenzymes in Thrombocytes in Various Diseases

Knudsen

Gormsen

1968

Scandinavian Journal of Haematology

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LDH isoenzymes in thrombocytes from 41 healthy subjects and 85 patients suffering from various diseases combined with changes in platelet function were studied. The isoenzymes were separated by disc acrylamide electrophoresis. In normal thrombocytes LDH 3 constituted around 41 per cent, LDH 2 around 32 per cent, LDH 1 around 12 per cent and LDH 4 around 14 per cent of the total amount, whereas only very little LDH 5 was found. The results have been evaluated statistically. No differences were found between younger and older healthy persons. In patients with hepatic cirrhosis and polycythaemia vera an increase in ratio between H and M units (increase in T‐values) was demonstrated. Significant lower T‐values were found in patients with cancer and acute phlebothrombosis. No relationship between isoenzyme parameters of thrombocytes and serum was found.

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“…Abnormal electrophoretic pattern of leucocyte enzymes have been found in patients with leukaemia. The alkaline phosphatase pattern may be grossly abnormal in chronic myelocytic leukaemia (CML) (Robinson et al 1965(Robinson et al , 1966 and the LDH pattern seems to show quantitative differences in CML as well as in chronic lymphatic leukaemia (CLL) (Bottomley et al 1966, Hule 1967, Rabinowitz & Dietz 1967. The present paper reports on abnormal phosphoglucomutase components in CLL.…”

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An Abnormal Phosphoglucomutase Component in Chronic Lymphatic Leukaemia

Lamm

Jensen

1970

Scandinavian Journal of Haematology

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An abnormal weak lymphocyte PGM isozyme was found in 5 out of 13 patients with CLL. Nothing conclusive can be said about its origin, but it deserves further attention and also indicates that the PGM pattern should be studied in other types of leukaemia. Care must be taken in the PGM phenotyping of leucocytes from patients with CLL and maybe also in patients with other haematological disorders, as a PGM31 individual may be misinterpreted as type PGM32–1.

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Lactate dehydrogenase isoenzyme patterns of human lymphatic and myeloid leukemic leukocytes

Cited by 8 publications

References 3 publications

Lactate dehydrogenase in synovial fluid diagnostic evaluation of total activity and isoenzyme patterns.

Lactate dehydrogenase in synovial fluid diagnostic evaluation of total activity and isoenzyme patterns.

Lactate Dehydrogenase Isoenzymes in Thrombocytes in Various Diseases

An Abnormal Phosphoglucomutase Component in Chronic Lymphatic Leukaemia

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