The present study was conducted on six cattle and two buffaloes with intestinal obstruction. Four cases had intestinal volvulus, three had intussusception and one case could have been volvulus or torsion. The haemato-biochemical parameters of these eight animals were studied, together with 10 healthy cows and 10 buffaloes as control. Rectal examination was helpful in subjective assessment of volvulus and intussusception but could not help in definitive diagnosis. Multiple dilated intestinal loops in pelvic cavity could be a diagnostic feature of intestinal volvulus. Complete anorexia, colic, loss of defecation, rumen atony, dehydration, tachycardia and tachypenia were the most pronounced clinical symptoms. The animals with intestinal obstruction had significantly higher packed cell volume, neutrophil count and significantly lower lymphocytic count. Reversal of neutrophil: lymphocyte ratio and moderate to marked left shift along with moderate to severe toxic changes in the neutrophils was a consistent finding. Serum biochemical analysis showed significant increase of aspartate aminotrasferase, fibrinogen, lactate and C-reactive protein levels and significant reduction in albumin, fibrinogen ratio, potassium, chloride, calcium and phosphorus levels. However, fibrinogen and fibrinogen ratio may be misleading and need to be interpreted cautiously.The rumen chloride level was increased. Peritoneal fluid changes were consistent with septic peritonitis, increased specific gravity, total protein, total cell count and number of neutrophils. Blood gas analysis revealed hypochloremic hypokalemic metabolic alkalosis with compensatory respiratory acidosis. So these biochemical changes should be taken into consideration while dealing with intestinal obstruction in cattle and buffaloes.