Lactiplantibacillus plantarum 22A-3 isolated from pickle suppresses ovalbumin-induced food allergy in BALB/c mice and 2,4-dinitrochlorobenzene-induced atopic dermatitis in NC/Nga mice

Mizuno, Masashi; Ohto, Nobuaki; Kuwahara, Hiroshige

doi:10.1016/j.jbiosc.2021.05.001

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…In addition, it is important to note that there is no uniform understanding of the trend of Lactobacillaceae in allergic individuals. Some studies have demonstrated that the abundance of Lactobacillaceae decreases in allergic individuals and that increasing the abundance of Lactobacillaceae has the effect of suppressing the allergic reaction. , …”

Section: Discussionmentioning

confidence: 99%

“…Some studies have demonstrated that the abundance of Lactobacillaceae decreases in allergic individuals and that increasing the abundance of Lactobacillaceae has the effect of suppressing the allergic reaction. 46,47 The altered intestinal flora in mice triggering OVA hypersensitivity may be associated with the downregulation of the aminoacyl-tRNA pathway. Compared to the control group, the serum metabolome of OVA mice showed a significant downregulation of the aminomethyl-tRNA synthesis metabolic pathway (p < 0.001).…”

Section: ■ Discussionmentioning

confidence: 99%

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Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice

et al. 2022

J. Agric. Food Chem.

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Gut microbiota plays an important role in the regulation of food allergy. However, the interactions between the gut flora and immune system are not well studied. Here, we obtained ovalbumin (OVA)-sensitive BALB/c mice, combined with serum untargeted metabolomics to investigate the mechanisms of the interactions. The serum metabolomics results showed that 17 serum metabolites were downregulated, enriched in the aminoacyl-tRNA biosynthesis pathway, whereas indole-3-propionic acid (IPA) was increased. Six operational taxonomic units (OTUs) at the family level were altered and correlated with immune endpoints. Combined metabolomic and microbiomic analyses revealed that IPA levels were correlated with differential bacterial OTUs and a positive correlation with Treg in splenic lymphocytes. These results suggest that the regulatory effects of intestinal flora on allergic responses may be achieved by metabolizing tryptophan to produce indole derivatives and the aminoacyl-tRNA biosynthesis pathway. The formation of OVA tolerance in mice may be related to the enrichment of Peptostreptococcaceae, Ruminococcaceae, and Lactobacillaceae.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice

et al. 2022

J. Agric. Food Chem.

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“…Method of manufacturing DNCB solutions and inducing AD. DNCB solution [Choi et al, 2011;Yang et al, 2015;Mizuno et al, 2021] was obtained by diluting DNCB with AOO solution (olive oil:100% acetone = 1:3). Hairless mice, with AD induced by applying DNCB, were assigned into three groups, namely, a Sham group and two experimental groups (PEMF 15 and 75 Hz group).…”

Section: Pemf Cores and Parametersmentioning

confidence: 99%

Evaluation of Atopic Dermatitis Improvement Caused by Low‐Level, Low‐Frequency Pulsed Electromagnetic Fields

Kim

Lee

et al. 2022

Bioelectromagnetics

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This study aimed to evaluate the effectiveness of using low‐level, low‐frequency pulsed electromagnetic field (LLLF_PEMF) stimulation to improve atopic dermatitis induced by 2,4‐dinitrochlorobenzene (DNCB). Twenty 6‐week‐old hairless mice were randomly divided into Normal (n = 5), PEMF 15 Hz (n = 5), PEMF 75 Hz (n = 5), and Sham (n = 5) groups. Following the onset of atopic dermatitis symptoms, PEMF groups (15 and 75 Hz) were stimulated with LLLF_PEMF (15 mT) for 8 h per day for 1 week. Sensory evaluation analysis revealed a significant difference between the PEMF 15 Hz group and Sham group (P < 0.05), but these differences were not visually obvious. While both the PEMF and Sham groups had atopic dermatitis lesions, lesion size was significantly smaller in the two PEMF groups than in the Sham group (P < 0.001). Additionally, changes in epithelial thickness because of skin inflammation significantly decreased for both PEMF groups, compared with the Sham group (P < 0.001). In conclusion, these results suggest that PEMF stimulation in vivo triggers electro‐chemical reactions that affect immune response. © 2022 Bioelectromagnetics Society.

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Levilactobacillus brevis IBARAKI‐TS3 Isolated From Pickles Promotes Production of Interleukin‐10 via Toll‐Like Receptor 2 in Human M2 Macrophages

Tobita,

Iwasa

2024

Cell Biochemistry & Function

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M2 macrophages play an important role in food allergy. Several studies have reported that lactic acid bacteria isolated from pickles exert antiallergic effects. We investigated the effects of several strains of lactic acid bacteria on the immune function of M2 macrophages. M2 macrophages differentiated from THP‐1 cell line by interleukin‐4 (IL‐4) and IL‐13 strongly expressed CD163, CD206, and HMOX1 mRNA. Levilactobacillus brevis IBARAKI‐TS3 (IBARAKI‐TS3) isolated from pickles was identified as a lactic acid bacterium that enhances the expressions of IL‐10 and EBI3 mRNA in M2 macrophages. IBARAKI‐TS3 induced the expression of genes involved in Toll‐like receptor (TLR) signaling, such as IRAK, mitogen‐activated protein kinases (MAPKs), and NF‐κB mRNA. IBARAKI‐TS3–induced IL‐10 production was suppressed by anti‐TLR2–neutralizing antibodies. Furthermore, the IBARAKI‐TS3–induced increase in IL‐10 levels was significantly reduced in TLR2–knockdown M2 macrophages compared to M2 macrophages. These results suggest that IBARAKI‐TS3 promotes of IL‐10 production via TLR2 in M2 macrophages.

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Lactiplantibacillus plantarum 22A-3 isolated from pickle suppresses ovalbumin-induced food allergy in BALB/c mice and 2,4-dinitrochlorobenzene-induced atopic dermatitis in NC/Nga mice

Cited by 6 publications

References 24 publications

Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice

Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice

Evaluation of Atopic Dermatitis Improvement Caused by Low‐Level, Low‐Frequency Pulsed Electromagnetic Fields

Levilactobacillus brevis IBARAKI‐TS3 Isolated From Pickles Promotes Production of Interleukin‐10 via Toll‐Like Receptor 2 in Human M2 Macrophages

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