Lactobacilli vaginal colonisation after oral consumption of Respecta® complex: a randomised controlled pilot study

Abstract

Consumption of L. acidophilus La-14, L. rhamnosus HN001 in combination with bovine lactoferrin leads to vaginal detection; even 1 week after consumption was stopped. This provides a basis for future studies on urogenital tract health.

Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus La-14 Attenuate Gardnerella vaginalis-Infected Bacterial Vaginosis in Mice

“…In addition, when L1 and L2 were orally administered as a mixture (PM), both L1 and L2 were detected in the vagina, as previously reported [18]. Administration of L1, L2, or PM reduced the number of GV detected in the vagina.…”

“…Oral administration of the probiotic mixture (PM; containing L1 and L2) resulted in the colonization of L1 and L2 in the vaginas of healthy women [18]. To determine whether these probiotics could also colonize the vaginas of mice, we orally or intravaginally administered PM or one of its probiotic components, L1 or L2, and assessed the vaginas from the presence of attached probiotics by qPCR (Figure 1).…”

“…Primers (Macrogen, Seoul, Korea) were prepared according to the method of de Alberti et al [18]: L1 forward: 5′-TGC TTG CAT CTT GAT TTA ATT TTG-3′, reverse: 5′-GGT TCT TGG ATY TAT GCG GTA TTA G-3′; L2 forward: 5′-TGC AAA GTG GTA GCG TAA GC-3′, reverse: 5′-CCT TTC CCT CAC GGT ACT G-3′; GV forward: 5′-TTA CTG GTG TAT CAC TGT AAG G-3′, reverse: 5′-CCG TCA CAG GCT GAA CAG T-3′; AV forward: 5′-GGT GAA GCA GTG GAA ACA CT-3′, reverse: 5′-ATT CGC TTC TGC TCG CGC A-3′; 16S rDNA forward: 5′-AGA GTT TGA TCC TGG CTC AG-3′, reverse: 5′-AAG GAG GTG WTC CAR CC-3′.…”

“…Strus et al reported that an orally administered probiotic mixture of Lactobacillus fermentum 57A, Lactobacillus plantarum 57B, and Lactobacillus gasseri 57C colonized the vagina of healthy women [17]. Arberti et al also reported that oral administration of a probiotic mixture (PM; Respecta ® ) consisting of Lactobacillus rhamnosus HN001 (L1), Lactobacillus acidophilus La-14 (L2), and lactoferrin RCXTM resulted in colonization of the probiotics in the vaginas of healthy women [18]. These results suggest that orally administered probiotics may ameliorate BV by colonizing the vagina.…”

Oral Probiotics Alter Healthy Feline Respiratory Microbiota

“…There is growing evidence that oral probiotics not only influence the GI tract directly, but also have immunomodulatory effects at distant sites, including the urogenital and respiratory tracts, oral cavity/dentition, mammary gland and skin. Although oral probiotics can prevent and/or decrease frequency or severity of diseases outside of the gastrointestinal tract (i.e., an indirect effect) (Alberda et al, 2007; Lappin et al, 2009; Di Nardo et al, 2014; Esposito et al, 2014; Lee et al, 2015; Zuccotti et al, 2015; Fuchs-Tarlovsky et al, 2016; Gruner et al, 2016; Vieira et al, 2016; Zamani et al, 2016), few studies have directly evaluated the appearance of orally administered probiotic species or changes in host microbial communities at distant sites (De Alberti et al, 2015; Mastromarino et al, 2015; Treven et al, 2015). A recent study in pre-menopausal women demonstrated an increase in orally administered probiotic species in vaginal swabs using qPCR analysis, setting the stage for use of oral probiotics to prevent or treat urogenital infections (Mezzasalma et al, 2017).…”