Lactobacillus acidophilus, L. plantarum, L. rhamnosus, and L. reuteri Cell-Free Supernatants Inhibit Candida parapsilosis Pathogenic Potential upon Infection of Vaginal Epithelial Cells Monolayer and in a Transwell Coculture System
            <i>In Vitro</i>

Spaggiari, Luca; Sala, Arianna; Ardizzoni, Andrea; Seta, Francesco De; Singh, Dhirendra Kumar; Gácser, Attila; Blasi, Elisabetta; Pericolini, Eva

doi:10.1128/spectrum.02696-21

Cited by 31 publications

(23 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, our findings hint that L. acidophilus is possibly associated with the abundance of specific bacterial species. This finding agrees with studies that have shown that L. acidophilus occurrence associates with a healthy cervix and a protective immune response, which may indicate its ability to enhance beneficial Lactobacillus species such as L. crispatus 41 – 44 . Alternatively, L. acidophilus occurrence may also lead to a decrease in the abundance of Lactobacillus species that generally dominate the CVM, facilitating the colonization by other microbes, an increase in diversity, and the transition to a different CST.…”

Section: Discussionsupporting

confidence: 92%

In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling

Molina

Andralojc

Huynen

et al. 2022

npj Biofilms Microbiomes

View full text Add to dashboard Cite

The cervicovaginal microbiome (CVM) correlates with women’s cervical health, and variations in its composition are associated with high-risk human papillomavirus (hrHPV) infection outcomes. Cervicovaginal microbes have been grouped into five community state types (CSTs) based on microbial community composition and abundance. However, studying the impact of CSTs in health and disease is challenging because the current sequencing technologies have limited confident discrimination between closely related and yet functionally different bacterial species. Circular probe-based RNA sequencing (ciRNAseq) achieves high-resolution microbiome profiling and therefore provides in-depth and unambiguous knowledge about the composition of the CVM. Based on ciRNAseq profiling of a large cohort of cervical smears (n = 541), we here define subgroups of CSTs I, III, and IV based on intra-CST differences with respect to abundances of Lactobacillus acidophilus (CSTs I-A vs. I-B and CSTs III-A vs. III-B), Lactobacillus iners (CSTs I-A vs. I-B and CSTs III-A vs. III-B), and Megasphaera genomosp type 1 (CSTs IV-A vs. IV-B). Our results further support the existence of subgroups of CST IV-C that are dominant for non-Lactobacillus species and have intermediate microbial diversity. We also show that CST V is associated with uninfected conditions, and CST IV-A associates with hrHPV-induced cervical disease. In conclusion, we characterized new subdivisions of cervicovaginal CSTs, which may further advance our understanding of women’s cervical health and hrHPV-related progression to disease.

show abstract

Section: Discussionsupporting

confidence: 92%

In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling

Molina

Andralojc

Huynen

et al. 2022

npj Biofilms Microbiomes

View full text Add to dashboard Cite

show abstract

“…By chromatogram analysis, the different elution peaks and their areas were used for semi-quantitative evaluation of the specific products detected in treated and untreated cells. Table 2 shows that untreated Candida cells secreted consistent amounts of tyrosol, in line with our recently published data [ 32 , 33 ].…”

Section: Resultssupporting

confidence: 90%

“…Certainly, the opposed effects observed on farnesol and tyrosol warrant further investigations; in any case, we may conclude that the regulation of these two AI is a key step in the accomplishment of antibiofilm effects by PomeGr. It should be noted that the present data are in line with recent papers demonstrating the complex modulation of Candida tyrosol/farnesol by other bioactive compounds [ 32 , 33 ].…”

Section: Discussionsupporting

confidence: 93%

“…PomeGr extract is known to be a complex mixture of components, such as polyphenols with a broad spectrum of activities, including antimicrobial, antioxidant and anti-inflammatory effects [ 31 , 32 , 33 , 34 , 35 ]. Initially, to establish the biochemical profile of the PomeGr extract used in this study, HPLC-ESI-MS analysis was performed and a representative chromatogram is shown in Figure 2 .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Pomegranate Extract Affects Fungal Biofilm Production: Consumption of Phenolic Compounds and Alteration of Fungal Autoinducers Release

Colombari

Tagliazucchi

Odorici

et al. 2022

IJERPH

Self Cite

View full text Add to dashboard Cite

Candida albicans expresses numerous virulence factors that contribute to pathogenesis, including its dimorphic transition and even biofilm formation, through the release of specific quorum sensing molecules, such as the autoinducers (AI) tyrosol and farnesol. In particular, once organized as biofilm, Candida cells can elude conventional antifungal therapies and the host’s immune defenses as well. Accordingly, biofilm-associated infections become a major clinical challenge underlining the need of innovative antimicrobial approaches. The aim of this in vitro study was to assess the effects of pomegranate peel extract (PomeGr) on C. albicans growth and biofilm formation; in addition, the release of tyrosol and farnesol was investigated. The phenolic profile of PomeGr was assessed by high-performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis before and after exposure to C. albicans. Here, we showed that fungal growth, biofilm formation and AI release were altered by PomeGr treatment. Moreover, the phenolic content of PomeGr was substantially hampered upon exposure to fungal cells; particularly pedunculagin, punicalin, punicalagin, granatin, di-(HHDP-galloyl-hexoside)-pentoside and their isomers as well as ellagic acid–hexoside appeared highly consumed, suggesting their role as bioactive molecules against Candida. Overall, these new insights on the anti-Candida properties of PomeGr and its potential mechanisms of action may represent a relevant step in the design of novel therapeutic approaches against fungal infections.

show abstract

“…Based on the above mentioned, the term "postbiotic-like" will be used in this review. This term has recently employed to refer the use of cell-free supernatants of lactic acid bacteria (Spaggiari et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Role of lactic acid bacteria and yeasts in sourdough fermentation during breadmaking: Evaluation of postbiotic-like components and health benefits

et al. 2022

View full text Add to dashboard Cite

Sourdough (SD) fermentation is a traditional biotechnological process used to improve the properties of baked goods. Nowadays, SD fermentation is studied for its potential health effects due to the presence of postbiotic-like components, which refer to a group of inanimate microorganisms and/or their components that confer health benefits on the host. Some postbiotic-like components reported in SD are non-viable microorganisms, short-chain fatty acids, bacteriocins, biosurfactants, secreted proteins/peptides, amino acids, flavonoids, exopolysaccharides, and other molecules. Temperature, pH, fermentation time, and the composition of lactic acid bacteria and yeasts in SD can impact the nutritional and sensory properties of bread and the postbiotic-like effect. Many in vivo studies in humans have associated the consumption of SD bread with higher satiety, lower glycemic responses, increased postprandial concentrations of short-chain fatty acids, and improvement in the symptoms of metabolic or gastrointestinal-related diseases. This review highlights the role of bacteria and yeasts used for SD, the formation of postbiotic-like components affected by SD fermentation and the baking process, and the implications of functional SD bread intake for human health. There are few studies characterizing the stability and properties of postbiotic-like components after the baking process. Therefore, further research is necessary to develop SD bread with postbiotic-related health benefits.

show abstract

Lactobacillus acidophilus, L. plantarum, L. rhamnosus, and L. reuteri Cell-Free Supernatants Inhibit Candida parapsilosis Pathogenic Potential upon Infection of Vaginal Epithelial Cells Monolayer and in a Transwell Coculture System In Vitro

Abstract: The incidence of VVC by non- albicans Candida (NAC) species, such as C. parapsilosis , is increasing. Treatment failure is common in NAC-VVC because some species are resistant or poorly susceptible to the antifungal agents normally employed.

Cited by 31 publications

References 39 publications

In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling

In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling

Pomegranate Extract Affects Fungal Biofilm Production: Consumption of Phenolic Compounds and Alteration of Fungal Autoinducers Release

Role of lactic acid bacteria and yeasts in sourdough fermentation during breadmaking: Evaluation of postbiotic-like components and health benefits

Contact Info

Product

Resources

About