Lactobacillus acidophilus La5, Bifidobacterium BB12, and Lactobacillus casei DN001 modulate gene expression of subset specific transcription factors and cytokines in peripheral blood mononuclear cells of obese and overweight people

Abstract

Probiotics are believed to have interaction with immune cells through sustained effects on gene expression of different cytokines and transcription factors. The present randomized doubled-blind controlled clinical trial was performed recruiting 75 individuals with BMI 25-35, who were randomly assigned to the following three groups: Group 1 (n = 25) who consumed regular yogurt as part of a low calorie diet [RLCD], group 2 (n = 25) who received probiotic yogurt with a LCD [PLCD] and group 3 (n = 25) who consumed probiotic yogurt without LCD [PWLCD] for 8 week. Participants in PLCD and PWLCD groups received 200 g/day yogurt containing Lactobacillus acidophilus La5, Bifidobacterium Bb12, and lactobacillus casei DN001 10(8) cfu/gr. The expression of the FOXP3, T-bet, GATA3, TNF-α, IFN-γ, TGF-β, and ROR-γt in PBMCs genes were assessed, before and after intervention. In three groups, ROR-γt expression was reduced (P = 0.007) and FOXP3 was increased (P < 0.001). The expression of TNFα, TGFβ, and GATA3 genes did not change among all groups after intervention. Interestingly, the expression of T-bet gene, which was significantly decreased in PLCD and PWLCD groups (P < 0.001), whereas gene expression of IFN-γ decreased in all three groups. Our results suggest that weight loss diet and probiotic yogurt had synergistic effects on T-cell subset specific gene expression in peripheral blood mononuclear cells among overweight and obese individuals.

Probiotic Foods and Supplements Interventions for Metabolic Syndromes: A Systematic Review and Meta-Analysis of Recent Clinical Trials

“…In the reviewed studies, only Hariri et al [54] reported a significant decrease of DBP and SBP when participants took Lactobacillus and Bifidobacterium foods or supplements. Other studies [50,52,57,64] reported no significant differences in blood pressure. Also, Agerholm-Larsen et al [61] suggested that there was a significant decrease in SBP and a significant increase in DBP between the intervention group and the control group.…”

“…In terms of sample size calculation, 8 RCT studies did not report any type of power calculations [42,47,49,50,52,54,55,61]. One study reported post hoc calculation [66].…”

“…Six reviewed studies showed significant differences in the waist circumference, hip circumference, or both [51-53, 55, 56, 59]. While other 5 studies found no significant changes in these anthropometric outcome measurements [42,48,50,52,54].…”

“…Four studies [42,[51][52][53] reported significant differences in BMI between intervention and control group. While the rest of the studies [54][55][56][57] did not find significant BMI changes after the intervention.…”

The Gut Bacteria-Driven Obesity Development

“…A recently published meta-analysis found only 4 high-quality, randomized controlled studies comparing the therapeutic efficacy of probiotics with placebo and using body weight loss as the main end-point (table 1) [76,77,78,79]. The analysis of the pooled data failed to demonstrate significant differences in body weight, body mass index and mean visceral fat mass between the probiotic group and controls, therefore suggesting that probiotics are ineffective on body weight modulation [80].…”