Lactobacillus paracasei R3 protects against dextran sulfate sodium (DSS)-induced colitis in mice via regulating Th17/Treg cell balance

Abstract

Inflammatory bowel diseases (IBD), mainly comprising ulcerative colitis (UC) and Crohn's Disease, are most often a polygenic disorder with contributions from the intestinal microbiome, defects in barrier function, and dysregulated host responses to microbial stimulation. Strategies that target the microbiota have emerged as potential therapies and, of these, probiotics have gained the greatest attention. Herein, we isolated a strain of Lactobacillus paracasei R3 (L.p R3) with strong biofilm formation ability from infant feces. Interestingly, we also found L.p R3 strain can ameliorate the general symptoms of murine colitis, alleviate inflammatory cell infiltration and inhibit Th17 while promote Treg function in murine dextran sulfate sodium (DSS)-induced colitis. Overall, this study suggested that L.p R3 strain significantly improves the symptoms and the pathological damage of mice with colitis and influences the immune function by regulating Th17/Treg cell balance in DSS-induced colitis in mice.

Alleviation of DSS-induced colitis in mice by a new-isolated Lactobacillus acidophilus C4

“…The mucophilic bacterium Akkermansia is a good example to protect the intestinal barrier by acetate and propionate production ( Hiippala et al, 2018 ). Unlike the results of other studies where strains alleviated DSS-induced colitis by a single effect ( Chen et al, 2015 ; Huang et al, 2021 ), we have demonstrated that the protective effect of L. acidophilus C4 for the repair of the intestinal mucous is achieved by relieving colon tissue damage, reducing the level of tissue inflammatory factors, and regulating the oxidative stress in mice. In addition, L. acidophilus C4 can maintain the homeostasis of the intestinal microenvironment by regulating the composition and abundance of the intestinal microbiota and the production of SCFAs.…”

Bioactive polysaccharides promote gut immunity via different ways

“…37,38 Inulin may promote Th17-type immune response by significantly upregulating ( p < 0.05) the abundance of Lactobacillus with Monoglobus to produce large amounts of SCFAs and significantly improving ( p < 0.05) nine disordered metabolic pathways. 40 Fucoidan is more similar to carrageenan in improving disordered metabolic pathways. Fucoidan exerts optimal metabolic disorder improvement by significantly inhibiting ( p < 0.05) all CTX immunosuppressive marker bacteria, significantly promoting the beneficial bacteria Rikenella , and significantly restoring ( p < 0.05) CD4/CD8 and upregulating TNF-α, IL-17, and IL-22.…”

Curcumin regulates the homeostasis of Th17/Treg and improves the composition of gut microbiota in type 2 diabetic mice with colitis

“…The immune homeostasis of Th17/Treg plays a key role in host health and its disruption often induces autoimmune diseases, including IBD (Huang et al, 2021) and DM (Zhang et al, 2021b). Th17 cells are pro-inflammatory, secreting large amounts of IL-17A upon activation, causing damage to the colonic mucosa (Owaga et al, 2015), killing pancreatic β-cells and enhancing insulin tolerance (Kiernan & Maciver, 2020).…”