Lactose Malabsorption in Two Sibling Rhesus Monkeys
(Macaco mulatta)

Short-chain fatty acids (SCFA) are considered to have important physiological functions. However, to prove this, SCFA must be determined, which is rather difficult as a lot of factors interfere with their production. This review focuses on the factors that influence the prediction of short-chain fatty acid formation in the large intestine of monogastric mammals. To mimic the in vivo situation, when predicting the amount of short-chain fatty acids produced from a certain substrate based on in vitro models, one has to estimate the amount of this substrate entering the large intestine, the retention time in the different parts of the large intestine, and the substrate fermentability. Instead of in vitro models, direct and indirect techniques may be used to measure short-chain fatty acid production in vivo. Direct techniques include the measurement of input and output or measuring differences in SCFA between portal and venous blood whereas indirect techniques measure the end products of fermentation. In this case, other factors have to be taken into account, including technical limitations and ethical considerations. In this review it is concluded that the choice for a method will rely on the purpose of the study taking into account the (dis)advantages of every method.

show abstract

Microbiome signature suggestive of lactose-intolerance in rhesus macaques (Macaca mulatta) with intermittent chronic diarrhea

Maaskant,

Voermans,

Levin

et al. 2024

anim microbiome

View full text Add to dashboard Cite

Background Chronic diarrhea is a common cause of mortality and morbidity in captive rhesus macaques ( Macaca mulatta ). The exact etiology of chronic diarrhea in macaques remains unidentified. The occurrence of diarrhea is frequently linked to dysbiosis within the gut microbiome. Research into microbiome signatures correlated with diarrhea in macaques have predominantly been conducted with single sample collections. Our analysis was based on the metagenomic composition of longitudinally acquired fecal samples from rhesus macaques with chronic diarrhea and clinically healthy rhesus macaques that were obtained over the course of two years. We aimed to investigate potential relationships between the macaque gut microbiome, the presence of diarrhea and diet interventions with a selection of commercially available monkey diets. Results The microbiome signature of macaques with intermittent chronic diarrhea showed a significant increase in lactate producing bacteria e.g. lactobacilli , and an increase in fermenters of lactate and succinate. Strikingly, two lactose free diets were associated with a lower incidence of diarrhea. Conclusion A lactose intolerance mechanism is suggested in these animals by the bloom of Lactobacillus in the presence of lactose resulting in an overproduction of intermediate fermentation products likely led to osmotically induced diarrhea. This study provides new insights into suspected microbiome-lactose intolerance relationship in rhesus macaques with intermittent chronic diarrhea. The integration of machine learning with metagenomic data analysis holds potential for developing targeted dietary interventions and therapeutic strategies and therefore ensuring a healthier and more resilient primate population. Supplementary Information The online version contains supplementary material available at 10.1186/s42523-024-00338-z.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Lactose Malabsorption in Two Sibling Rhesus Monkeys (Macaco mulatta)

Cited by 3 publications

References 1 publication

Impact of concerning excipients on animal safety: insights for veterinary pharmacotherapy and regulatory considerations

Impact of concerning excipients on animal safety: insights for veterinary pharmacotherapy and regulatory considerations

Prediction of In Vivo Short-Chain Fatty Acid Production in Hindgut Fermenting Mammals: Problems and Pitfalls

Microbiome signature suggestive of lactose-intolerance in rhesus macaques (Macaca mulatta) with intermittent chronic diarrhea

Contact Info

Product

Resources

About