Laminin Modified Infection‐Preventing Collagen Membrane Containing Silver Sulfadiazine–Hyaluronan Microparticles

Abstract

The newly developed laminin modified infection-preventing collagen membrane consists of a 3 component laminate, comprising 2 outer collagen layers and a central laminin layer. The 2 outer collagen layers (dense and porous layers) were fabricated by air-drying and freeze drying, respectively, and the laminin layer was formed by a straightforward liquid coating method. In addition, hyaluronan based microparticles containing silver sulfadiazine (AgSD) were incorporated into the 2 collagen layers (AgSD content 50 microg/cm2). Laminin coated collagen surfaces did not promote fibroblast attachment but showed a retarded fibroblast proliferation rate and an increased rate of collagen synthesis versus pure collagen surfaces. In an animal study, a laminin coating on a nonmedicated collagen membrane significantly increased both wound size reduction and vessel proliferation 7 days after application versus polyurethane film. Interestingly, the laminin coated AgSD medicated collagen membrane demonstrated higher wound size reduction and vessel proliferation and lower inflammation than the polyurethane control, suggesting that the laminin AgSD medicated collagen membrane substantially improves dermal wound healing.

Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends

“…Current strategies for tissue regenerative wound dressings have also aimed at the development of implantable matrices that mimic the natural tissue. In this context, natural proteins such as collagen (Grzybowski et al 1997;Lee et al 2002;Ruszczak 2003;Wu et al 2003;Sripriya et al 2004), gelatin (Chang et al 2003b), soy protein (Silva et al 2003(Silva et al , 2005b, casein (Silva et al 2003) and silk fibroin (Santin et al 1999) have been studied separately or as composite materials in a template to be used in skin tissue engineering due to their similarity with the living tissues. An important issue for the treatment of an infected wound is to sustain sufficient drug concentration at the site of infection.…”

“…An important issue for the treatment of an infected wound is to sustain sufficient drug concentration at the site of infection. Therefore, different types of medicated collagen dressings with antibiotics were developed (Lee et al 2002;Sripriya et al 2004). Lee et al (2002) have developed an infection-preventing bilayered membrane by combining silver sulphadiazine and a laminin-modified collagen membrane, which was shown to facilitate the dermal wound healing process.…”

Triphala Incorporated Collagen Sponge—A Smart Biomaterial for Infected Dermal Wound Healing

“…This has been overcome successfully by topical application of drugs, and collagen dressings with antibiotics have also been developed to control infection [7][8][9]. Development of bacterial resistance to antibiotics continues, and the use of new antimicrobial agents is increasing [10].…”

Air‐dried 3D‐collagen–chitosan biocomposite scaffold for tissue engineering application

“…Stress–strain measurement was carried out on an autograph (recorder) at a crosshead speed of 20 mm/min at 25°C and at a relative humidity of 65 and 85%. Percentage of elongation at break was measured using a universal testing machine (INSTRON model 1405) according to Vogel at an extension rate of 5 mm/min [26].…”