Langdon durum disomic substitution lines and aneuploid analysis in tetraploid wheat

Joppa, L. R.; Williams, N. D.

doi:10.1139/g88-038

Cited by 158 publications

(126 citation statements)

References 9 publications

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“…Diploid Triticum monococcum subsp monococcum line DV92 (A m genome) and tetraploid Triticum turgidum subsp durum cv Langdon (AB genome) were used. The copy numbers and chromosomal locations of restriction fragment length polymorphism probes were determined by DNA gel blot hybridization using aneuploid nullitetrasomic lines of hexaploid wheat, Chinese Spring (Sears, 1966), and Langdon/Chinese Spring substitution lines in which chromosomes of Langdon were substituted by the Chinese Spring D genome homologs (Joppa and Williams, 1988).…”

Section: Plant Materialsmentioning

confidence: 99%

Rapid Genome Divergence at Orthologous Low Molecular Weight Glutenin Loci of the A and Am Genomes of Wheat[W]

et al. 2003

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To study genome evolution in wheat, we have sequenced and compared two large physical contigs of 285 and 142 kb covering orthologous low molecular weight (LMW) glutenin loci on chromosome 1AS of a diploid wheat species ( Triticum monococcum subsp monococcum ) and a tetraploid wheat species ( Triticum turgidum subsp durum ). Sequence conservation between the two species was restricted to small regions containing the orthologous LMW glutenin genes, whereas Ͼ 90% of the compared sequences were not conserved. Dramatic sequence rearrangements occurred in the regions rich in repetitive elements. Dating of long terminal repeat retrotransposon insertions revealed different insertion events occurring during the last 5.5 million years in both species. These insertions are partially responsible for the lack of homology between the intergenic regions. In addition, the gene space was conserved only partially, because different predicted genes were identified on both contigs. Duplications and deletions of large fragments that might be attributable to illegitimate recombination also have contributed to the differentiation of this region in both species. The striking differences in the intergenic landscape between the A and A m genomes that diverged 1 to 3 million years ago provide evidence for a dynamic and rapid genome evolution in wheat species.

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Section: Plant Materialsmentioning

confidence: 99%

Rapid Genome Divergence at Orthologous Low Molecular Weight Glutenin Loci of the A and Am Genomes of Wheat[W]

et al. 2003

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“…A complete set of substitution lines with D-genome chromosomes replacing each of the 14 chromosome pairs of cv. Langdon (Joppa and Williams 1988) was used to assign selected BAC clones to the A or B genomes.…”

Section: Plant Materialsmentioning

confidence: 99%

“…In many cases it is important to determine the genome source of the selected BACs, a task that is facilitated by the availability of substitution lines of each of the 14 Langdon chromosomes by the homeologous chromosome from the D genome (Joppa and Williams 1988). The high level of restriction fragment length polymorphisms (RFLPs) between the different genomes can be used to assign BACs to the A or B genomes.…”

Section: Determination Of the Genome Source Of Selected Bacs Using Chmentioning

confidence: 99%

Construction and characterization of a half million clone BAC library of durum wheat (Triticum turgidum ssp. durum)

et al. 2003

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Durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, genomes AB) is an economically important cereal used as the raw material to make pasta and semolina. In this paper we present the construction and characterization of a bacterial artificial chromosome (BAC) library of tetraploid durum wheat cv. Langdon. This variety was selected because of the availability of substitution lines that facilitate the assignment of BACs to the A and B genome. The selected Langdon line has a 30-cM segment of chromosome 6BS from T. turgidum ssp. dicoccoides carrying a gene for high grain protein content, the target of a positional cloning effort in our laboratory. A total of 516,096 clones were organized in 1,344 384-well plates and blotted on 28 high-density filters. Ninety-eight percent of these clones had wheat DNA inserts (0.3% chloroplast DNA, 1.4% empty clones and 0.3% empty wells). The average insert size of 500 randomly selected BAC clones was 131 kb, resulting in a coverage of 5.1-fold genome equivalents for each of the two genomes, and a 99.4% probability of recovering any gene from each of the two genomes of durum wheat. Six known copy-number probes were used to validate this theoretical coverage and gave an estimated coverage of 5.8-fold genome equivalents. Screening of the library with 11 probes related to grain storage proteins and starch biosynthesis showed that the library contains several clones for each of these genes, confirming the value of the library in characterizing the organization of these important gene families. In addition, characterization of fingerprints from colinear BACs from the A and B genomes showed a large differentiation between the A and B genomes. This library will be a useful tool for evolutionary studies in one of the best characterized polyploid systems and a source of valuable genes for wheat. Clones and high-density filters can be requested at

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“…A D-genome disomic substitution series of durum wheat (Joppa and Williams 1988) was used for the chromosomal assignment of an amplified STS marker fragment. The following accessions were used to study polymorphisms in the STS alleles: 27 accessions of wild emmer wheat (T. dicoccoides), 61 of cultivated emmer wheat (T. durum, T. turgidum, T. palaeocolchicum, T. pyramidale, T. polonicum, T. ispahanicum, T. carthricum, T. abyssinicum, T. dicoccum), 26 of wild timopheevi wheat (T. araraticum) and nine of cultivated timopheevi wheat Table 1).…”

Section: Methodsmentioning

confidence: 99%

Single nucleotide polymorphisms in an STS region linked to the Ncc-tmp1A locus are informative for characterizing the differentiation of chromosome 1A in wheat.

et al. 2001

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Homoeoalleles of Ncc confer nucleus-cytoplasm (NC) compatibility on NC hybrids of wheat with the D plasmon of Aegilops squarrosa. To dissect the chromosomal region containing Ncc, a RAPD marker linked to the Ncc-tmp1A locus, which is located on chromosome 1A of T. timopheevi, was sequenced and converted to a PCRbased sequence-tagged-site (STS) marker. Five single nucleotide polymorphisms (SNPs) between T. timopheevi and T. turgidum. were detected in a 509-bp genomic DNA fragment. Based on the SNPs, the STS alleles in 164 accessions from emmer wheat, timopheevi wheat and two einkorn wheats, T. urartu and T. boeoticum were surveyed by PCR-RFLP analysis. The sequence comparisons and PCR-RFLP analyses revealed nine alleles based on six SNPs. These SNPs were highly conserved within each group of wheat, and all groups could be distinguished by particular combinations of the SNPs. All accessions of T. urartu had one unique STS allele as compared with the others. Our results indicate that the SNPs in the STS marker linked to the Ncc-tmp1A locus would be informative for studies of the differentiation of chromosome 1A in wheat.

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Langdon durum disomic substitution lines and aneuploid analysis in tetraploid wheat

Cited by 158 publications

References 9 publications

Rapid Genome Divergence at Orthologous Low Molecular Weight Glutenin Loci of the A and Am Genomes of Wheat[W]

Rapid Genome Divergence at Orthologous Low Molecular Weight Glutenin Loci of the A and Am Genomes of Wheat[W]

Construction and characterization of a half million clone BAC library of durum wheat (Triticum turgidum ssp. durum)

Single nucleotide polymorphisms in an STS region linked to the Ncc-tmp1A locus are informative for characterizing the differentiation of chromosome 1A in wheat.

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