Lanthanide Complexes and Quantum Dots: A Bright Wedding for Resonance Energy Transfer

Charbonnière, Loı̈c J.; Hildebrandt, Niko

doi:10.1002/ejic.200800332

Cited by 106 publications

(94 citation statements)

References 96 publications

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“…5C) (11, 90). Furthermore, by using long-lifetime fluorophores, such as lanthanide dyes and time-gated fluorescence spectroscopy (91), it is possible to discard the initial short-lifetime autofluorescence burst and detect only the long-lifetime fluorescence signal that is reporting on the target. Another exciting prospect is in vivo photoacoustic sensing, which combines NIR excitation with ultrasonic detection based on the photoacoustic effect and yields higher spatial resolution and deeper tissue penetration than fluorescence techniques.…”

Section: Moving Toward Applicationsmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

904

686

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Colloidal nanoparticle biosensors have received intense scientific attention and offer promising applications in both research and medicine. We review the state of the art in nanoparticle development, surface chemistry, and biosensing mechanisms, discussing how a range of technologies are contributing toward commercial and clinical translation. Recent examples of success include the ultrasensitive detection of cancer biomarkers in human serum and in vivo sensing of methyl mercury. We identify five key materials challenges, including the development of robust mass-scale nanoparticle synthesis methods, and five broader challenges, including the use of simulations and bioinformatics-driven experimental approaches for predictive modeling of biosensor performance. The resultant generation of nanoparticle biosensors will form the basis of high-performance analytical assays, effective multiplexed intracellular sensors, and sophisticated in vivo probes.Evolution has given rise to organisms of staggering complexity. Our now extensive knowledge of biological systems pales in comparison to the remaining mysteries. Unraveling these requires tools that probe the molecular machinery of life and provide detailed feedback on complex networks of subtle interactions. Such tools are cornerstones of biomedical research and practice, and improvements in these lead directly to a better understanding of fundamental biology, monitoring of health, and diagnosis of disease. Colloidal nanoparticle biosensors are a class of biological probe that will not only yield improved biological sensing but also provide a step change in our ability to probe the biomolecular realm. Nanoparticles can act as high-performance sensors because nanomaterials exhibit unique and useful behaviors not present in their bulk form: for example, bright tunable fluorescence from semiconductor nanoparticles and localized surface plasmon resonance (LSPR) phenomena in metallic nanoparticles. These particles exhibit intense responses to incident light (or other stimuli), and the ability to modulate this response by interaction with target analytes makes them excellent biosensor signal transducers. Outputs can be quantitative or qualitative depending on the functionality of

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Section: Moving Toward Applicationsmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

904

686

View full text Add to dashboard Cite

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“…FRET has been reported for nanocomposites made with dyes attached by physisorption [40] by ligand exchange [25,41,42] and by covalent bonds [3,15,27]. Although all three techniques yield the desired short separation distance between dye and nanoparticle, the strong covalent bond is best able to withstand subsequent processing steps.…”

Section: Selection Of Optical Antenna and Matching With Sensitizing Ionmentioning

confidence: 99%

“…When the distance from the optical antenna to the nanoparticle is on the order of 5 nm, the energy of the light captured by the optical antenna can be transferred to the nanoparticle by Förster Resonance Energy Transfer (FRET), a nonradiative mode of energy transfer proceeding via a short-range dipole-dipole interaction [26,27]. The optical antenna concept has been applied to dye-induced downconversion [15,27] and is the driving force for dyesensitized solar cells [28].…”

Section: Introductionmentioning

confidence: 99%

“…The optical antenna concept has been applied to dye-induced downconversion [15,27] and is the driving force for dyesensitized solar cells [28]. Recently, Zou et al [25] applied the optical antenna concept to upconversion, reporting a dye-induced upconversion with an activation bandwidth of ∼100 nm (versus <20 nm for direct atomic absorption) and a 1,100-fold increase in peak upconversion efficiency.…”

Section: Introductionmentioning

confidence: 99%

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Visible Discrimination of Broadband Infrared Light by Dye-Enhanced Upconversion in Lanthanide-Doped Nanocrystals

Dupuy

Allen

Williams

et al. 2014

Journal of Nanotechnology

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Optical upconversion of near infrared light to visible light is an attractive way to capture the optical energy or optical information contained in low-energy photons that is otherwise lost to the human eye or to certain photodetectors and solar cells. Until the recent application of broadband absorbing optical antennas, upconversion efficiency in lanthanide-doped nanocrystals was limited by the weak, narrow atomic absorption of a handful of sensitizer elements. In this work, we extend the role of the optical antenna to provide false-color, visible discrimination between bands of infrared radiation. By pairing different optical antenna dyes to specific nanoparticle compositions, unique visible emission is associated with different bands of infrared excitation. In one material set, the peak emission was increased 10-fold, and the width of the spectral response was increased more than 10-fold.

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“…Using acceptors such as quantum dots (QD) and phycobiliproteins (PE), which possess significantly higher absorption and molar extinction coefficients, R 0 values of 90 to 100 Å have been measured when using lanthanide donors. [18][19][20] Therefore, in principle, it should be possible to monitor FRET at distances less than 150 Å under practical experimental conditions when using acceptable donor-acceptor pairs. However, it should be noted that both chelated lanthanide donors and acceptors, such as QD and PE molecules, are significantly larger than conventional organic acceptor molecules.…”

mentioning

confidence: 99%

Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores: enhanced transfer in the presence of multiple acceptors

et al. 2012

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Abstract. Using commercially available organic fluorophores, the current applications of Förster (fluorescence) resonance energy transfer (FRET) are limited to about 80 Å. However, many essential activities in cells are spatially and/or temporally dependent on the assembly/disassembly of transient complexes consisting of large-size macromolecules that are frequently separated by distances greater than 100 Å. Expanding the accessible range for FRET to 150 Å would open up many cellular interactions to fluorescence and fluorescence-lifetime imaging. Here, we demonstrate that the use of multiple randomly distributed acceptors on proteins/antibodies, rather than the use of a single localized acceptor, makes it possible to significantly enhance FRET and detect interactions between the donor fluorophore and the acceptor-labeled protein at distances greater than 100 Å. A simple theoretical model for spherical bodies that have been randomly labeled with acceptors has been developed. To test the theoretical predictions of this system, we carried out FRET studies using a 30-mer oligonucleotide-avidin system that was labeled with the acceptors DyLight649 or Dylight750. The opposite 5′-end of the oligonucleotide was labeled with the Alexa568 donor. We observed significantly enhanced energy transfer due to presence of multiple acceptors on the avidin protein. The results and simulation indicate that use of a nanosized body that has been randomly labeled with multiple acceptors allows FRET measurements to be extended to over 150 Å when using commercially available probes and established protein-labeling protocols.

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Lanthanide Complexes and Quantum Dots: A Bright Wedding for Resonance Energy Transfer

Cited by 106 publications

References 96 publications

Colloidal nanoparticles as advanced biological sensors

Colloidal nanoparticles as advanced biological sensors

Visible Discrimination of Broadband Infrared Light by Dye-Enhanced Upconversion in Lanthanide-Doped Nanocrystals

Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores: enhanced transfer in the presence of multiple acceptors

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