Large deformation of living cells using laser traps

Lim, Chwee Teck; Dao, Ming; Suresh, S.; Sow, Chorng Haur; Chew, K. T.

doi:10.1016/j.actamat.2003.12.028

Cited by 151 publications

(135 citation statements)

References 23 publications

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“…The change in mechanical properties causes serious impairment of blood flow and results in severe anemia, coma, or even death. Suresh and coworkers [269][270][271][272] studied the mechanical response of living cells. Ingenious experimental techniques developed in collaboration with Lim enabled establishing the load extension response of a single cell.…”

Section: Mechanical Testingmentioning

confidence: 99%

Biological materials: Structure and mechanical properties

Meyers

Chen

Lin

et al. 2008

Progress in Materials Science

2,167

1,129

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Most natural (or biological) materials are complex composites whose mechanical properties are often outstanding, considering the weak constituents from which they are assembled. These complex structures, which have risen from hundreds of million years of evolution, are inspiring Materials Scientists in the design of novel materials.Their defining characteristics, hierarchy, multifunctionality, and self-healing capability, are illustrated. Self-organization is also a fundamental feature of many biological materials and the manner by which the structures are assembled from the molecular level up. The basic building blocks are described, starting with the 20 amino acids and proceeding to polypeptides, polysaccharides, and polypeptides-saccharides. These, on their turn, compose the basic proteins, which are the primary constituents of 'soft tissues' and are also present in most biominerals. There are over 1000 proteins, and we describe only the principal ones, with emphasis on collagen, chitin, keratin, and elastin. The 'hard' phases are primarily strengthened by minerals, which nucleate and grow in a biomediated environment that determines the size, shape and distribution of individual crystals. The most important mineral phases are discussed: hydroxyapatite, silica, and aragonite.Using the classification of Wegst and Ashby, the principal mechanical characteristics and structures of biological ceramics, polymer composites, elastomers, and cellular materials are presented. Selected systems in each class are described with emphasis on the relationship between their structure and mechanical response. A fifth class is added to this: functional biological materials, which have a structure developed for a specific function: adhesion, optical properties, etc.An outgrowth of this effort is the search for bioinspired materials and structures. Traditional approaches focus on design methodologies of biological materials using conventional synthetic 0079-6425/$ -see front matter Ó

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Section: Mechanical Testingmentioning

confidence: 99%

Biological materials: Structure and mechanical properties

Meyers

Chen

Lin

et al. 2008

Progress in Materials Science

2,167

1,129

View full text Add to dashboard Cite

show abstract

“…Guck et al (2000) calculated the stress distribution generated from two divergent counterpropagating beams around a single cell but the cell deformation was obtained from experimental measurements. Dao et al (2003), Lim et al (2004a) and Liu et al (2006) presented numerical simulations, based on mechanical models for two-bead attached RBCs, while Bareil et al (2006) numerically simulated the deformation of RBCs by OS. A model using the concept of Euler buckling instability has been used to capture the essential physics of RBC folding in an optical trap (Ghosh et al 2006).…”

Section: Optical-mechanical Interplaymentioning

confidence: 99%

Optical tweezers for single cells

Zhang

Liu

2008

J. R. Soc. Interface.

674

441

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Optical tweezers (OT) have emerged as an essential tool for manipulating single biological cells and performing sophisticated biophysical/biomechanical characterizations. Distinct advantages of using tweezers for these characterizations include non-contact force for cell manipulation, force resolution as accurate as 100 aN and amiability to liquid medium environments. Their wide range of applications, such as transporting foreign materials into single cells, delivering cells to specific locations and sorting cells in microfluidic systems, are reviewed in this article. Recent developments of OT for nanomechanical characterization of various biological cells are discussed in terms of both their theoretical and experimental advancements. The future trends of employing OT in single cells, especially in stem cell delivery, tissue engineering and regenerative medicine, are prospected. More importantly, current limitations and future challenges of OT for these new paradigms are also highlighted in this review.

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“…The shear modulus of discotic RBCs were measured as ~ 10 μN/m (Dao, Lim et al,2003). Using optical twezers system with a high power laser, the shear modulus values of RBCs under large deformation (corresponding to 400 pN) was measured as 11-18 μN/m while initial values were 19-30 μN/m, showing hyperelastic constitutive response (Lim, Dao et al,2004). Optical stretcher, a variant of optical tweezers, uses two diverging laser beams from opposite directions (Guck, Ananthakrishnan et al,2001).…”

Section: Optical Tweezersmentioning

confidence: 99%