Jet noise is a common problem in minimum quantity lubrication (MQL) technology. This should be given great attention because of its serious impacts on the physical and mental health of the operators. In this study, a micro-grooved nozzle is proposed based on the noise reduction concept of biological micro-grooves. The flow field and acoustic characteristics of an original nozzle and a micro-grooved nozzle were investigated numerically to help better understand the noise reduction mechanism. The reasons for noise generation and the effects of the length (L), width (W) and depth (δ) of the micro-grooves on noise reduction were analyzed. It was found that jet noise is generated by the large-scale vortex ring structure and the pressure fluctuations caused by its motion. The overall sound pressure level (OASPL) decreased with the increases in W and δ, and increased with the increase in L. Among of them, δ has the greatest effect on noise reduction. The maximum noise reduction achieved was 6.66 dB, as verified by the OASPL test. Finally, the noise reduction mechanism was discussed in terms of the flow field, vorticity and the frequency characteristics. Micro-grooves can enhance the mixing of airflow inside the nozzle and accelerate the process of large-scale vortices breaking into smaller-scale vortices. It also reduces the sound pressure level (SPL) of middle frequencies, as well as the SPL of high frequencies on specific angles.