Large‐scale isolation of high‐purity anthocyanin monomers from mulberry fruits by combined chromatographic techniques

Abstract

Anthocyanins have attracted attention over the past several decades because of their beneficial health effects. In this research, a strategy combining column chromatography and high-speed countercurrent chromatography was developed for the separation of high-purity anthocyanin monomers from mulberry fruits. After purification using Amberlite XAD-7HP column with 80% ethanol (0.1% HCl), a fraction of anthocyanins mixtures with a purity of 68.6% was obtained. High-speed countercurrent chromatography with a biphasic solvent system of n-butanol/methyl tert-butyl ether/acetonitrile/water/trifluoroacetic acid (30:10:10:50:0.05, v/v) was used to separate the anthocyanin monomers. Three monomers of delphinidin-3-O-rutinoside, cyanidin-3-O-rutinoside, and cyanidin-3-O-glucoside were obtained, and identified by H and C NMR spectroscopy and high-performance liquid chromatography with electrospray ionization-mass spectrometry. The method developed in this work can be used to conduct large-scale separations of anthocyanin monomers from mulberry fruits and other plants.

Inhibitory Effect on Acrolein by Cyanidin-3-O-glucoside and Its Acrolein Adducts from the Pigment of Mynica Red

“…It was preliminarily proven that C3G–ACR was a mono-ACR adduct of C3G. Based on the known NMR data of C3G, , the 13 C NMR spectra of C3G–ACR showed a carbon skeleton similar to that of C3G except for three additional carbon signals at δ C 95.15, δ C 26.70, and δ C 16.32 (Table ). Additionally, it was also observed from its 1 H NMR spectrum that the proton signal of C3G at δ H 6.64 (1H, d, J = 1.9 Hz) disappeared, and additional proton signals appeared at δ H 2.81 (2H, m), δ H 2.17 (2H, m), and δ H 5.82 (1H, m), indicating that the ACR group was joined at C-6 in the A ring of C3G.…”

Valorization Options of Strawberry Extrudate Agro-Waste. A Review

“…Peonidin-3-glucoside and malvidin-3-glucoside were obtained from grapes in a single step, while in a second step, cyanidin-3-glucoside was isolated [72]. In another research, the separation of anthocyanin monomers of high purity from mulberry fruits was developed [73].…”

Enhancing the Potential Exploitation of Food Waste: Extraction, Purification, and Characterization of Renewable Specialty Chemicals from Blackcurrants (Ribes nigrum L.)

“…10,13,[19][20][21] Unrefined simple extracts or tinctures, although often colored, contain only low levels of anthocyanins and are of limited use, however approaches for the preparation of extracts containing relatively high levels of anthocyanins for large scale applications have been reported. [22][23][24] Extraction and semi-purification of polyphenols Dried blackcurrant epicarp (30 g) was immersed in mL water acidified with 0.01% v/v conc. HCl and stirred gently by magnetic follower at room temperature for 2 hours.…”