Large-Scale Production of Liposomes by A Microfluidizer

Nanoengineered Drug Delivery Systems for Enhancing Antibiotic Therapy

“…Since their inception, the most commonly applied methods used for preparing liposomes include thin‐film hydration, reversed‐phase evaporation, solvent injection techniques, and detergent dialysis . Materials used for preparation, classification, and different techniques for the preparation of liposomes can be found elsewhere in the literature …”

Liposomes Size Engineering by Combination of Ethanol Injection and Supercritical Processing

“…The technique was developed by Batzri and Korn and optimized by several authors up to the pilot scale, because it was reported as a simple, rapid, and easy to scale up method. The liposomes size, encapsulation efficiency lamellarity, and stability are well controlled and reproducible …”

“…The liposomes size, encapsulation efficiency lamellarity, and stability are well controlled and reproducible. 14,15 Although various techniques have been developed for the bench-scale liposome formation, the broad application of liposomes in drug delivery is still hindered by scale-up issues. Liposome formation processes should be validated according to good manufacturing practice protocols prior to commercialization.…”

Preparation and Characterization of Liposomal Delivery System of Natural Heme Protein

“…Today, one of the main industrial methods for obtaining small unilamellar liposome vesicles with active pharmaceutical ingredients included is high-pressure homogenization. The advantage of this method, in comparison with the methods of ultrasound, freeze-thaw, injection, etc., is the standard approaches and potential scaling ability to large-scale production, high yield, minimal oxidation and hydrolysis of phospholipids, integrity of the drug, stability of the resulting liposomes and control over key parameters in the technology process [30][31][32]. The selected homogenization mode may allow obtaining liposomes of standard composition, the bulk of which is represented by particles no larger than 200 nm.…”