Larval development and metamorphic behaviour of the subtropical spionid polychaete Pseudopolydora vexillosa

Mok, Flora Siu Yan; Thiyagarajan, Vengatesen; Qian, Pei‐Yuan

doi:10.1016/j.jembe.2007.12.029

Cited by 9 publications

(19 citation statements)

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“…In most spionid polychaetes, metamorphosis includes the loss of the larval swimming chaete, the redirection of the palps, and the development of a ciliated groove along the palps, all of which can be completed in a very short time [11]. In this study, we hypothesized that post-translational modification, particularly protein phosphorylation, is involved in regulating morphogenesis during larval metamorphosis.…”

Section: 0 Discussionmentioning

confidence: 99%

“…In general, marine invertebrate larvae have evolved to undergo speedy metamorphosis to minimize the time that they are most vulnerable to predation, which is the period after losing their larval structures and before complete settlement in the new habitat [ 1 ]. In most spionid polychaetes, metamorphosis includes the loss of the larval swimming chaete, the redirection of the palps, and the development of a ciliated groove along the palps, all of which can be completed in a very short time [ 11 ]. In this study, we hypothesized that post-translational modification, particularly protein phosphorylation, is involved in regulating morphogenesis during larval metamorphosis.…”

Section: 0 Discussionmentioning

confidence: 99%

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Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa

et al. 2011

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BackgroundThe metamorphosis of the spionid polychaete Pseudopolydora vexillosa includes spontaneous settlement onto soft-bottom habitats and morphogenesis that can be completed in a very short time. A previous study on the total changes to the proteome during the various developmental stages of P. vexillosa suggested that little or no de novo protein synthesis occurs during metamorphosis. In this study, we used multicolor fluorescence detection of proteins in 2-D gels for differential analysis of proteins and phosphoproteins to reveal the dynamics of post-translational modification proteins in this species. A combination of affinity chromatography, 2D-PAGE, and mass spectrometry was used to identify the phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles.ResultsWe reproducibly detected 210, 492, and 172 phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles, respectively. The highest percentage of phosphorylation was observed during the competent larval stage. About 64 stage-specific phosphoprotein spots were detected in the competent stage, and 32 phosphoproteins were found to be significantly differentially expressed in the three stages. We identified 38 phosphoproteins, 10 of which were differentially expressed during metamorphosis. These phosphoproteins belonged to six categories of biological processes: (1) development, (2) cell differentiation and integrity, (3) transcription and translation, (4) metabolism, (5) protein-protein interaction and proteolysis, and (6) receptors and enzymes.ConclusionThis is the first study to report changes in phosphoprotein expression patterns during the metamorphosis of the marine polychaete P. vexillosa. The higher degree of phosphorylation during the process of attaining competence to settle and metamorphose may be due to fast morphological transitions regulated by various mechanisms. Our data are consistent with previous studies showing a high percentage of phosphorylation during competency in the barnacle Balanus amphitrite and the bryozoan Bugula neritina. The identified phosphoproteins may play an important role during metamorphosis, and further studies on the location and functions of important proteins during metamorphosis are warranted.

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confidence: 99%

Section: 0 Discussionmentioning

confidence: 99%

Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa

et al. 2011

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“…From these cultures, pre-competent larvae, competent larvae, and newly metamorphosed juveniles of Pseudopolydora vexillosa , (Fig. 1 ) were collected according to Mok et al [ 52 ] Briefly, larvae are released from the adult's tube when they have 3 fully developed setigers then develop for 6 days at 28°C until they become competent at the 13-14 setiger stage. Larvae will then settle and metamorphose within 24 hours when provided sediment substratum [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa

2009

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BackgroundWhile the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle.ResultsUnlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase).ConclusionsThis was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.

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“…Some factors such as ingestion rates, digestive rates, assimilation efficiency or differences in nutritional chemistry affect larval growth (Pechenik and Fisher, 1979). The nutritional value of any algal species for a particular organism depends not only on its chemical component, but also on the cell size (Brown and Robert, 2002;Mok et al, 2008). The biochemical component of the algal diet as a food for larvae also influences energy storage (Hansen, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…The biochemical component of the algal diet as a food for larvae also influences energy storage (Hansen, 1993). The amount of energy accumulated during the pre-metamorphosis stage influences larval settlement and ability to metamorphose successfully (Jarrett, 2003;Mok et al, 2008). Moreover, for species with feeding larvae, the type of food or food quality has a direct effect on larvae growth and energy reserves (Thiyagarajan and Qian, 2003).…”

Section: Discussionmentioning

confidence: 99%

Influence of Different Algal Diets on Larval Growth Rates in the Marine Serpulidae Polychaete Worm Spirobranchus kraussii

Lavajoo

2019

Croatian Journal of Fisheries

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Effects of food availability on larval growth and survival of Spirobranchus kraussii were studied by feeding larvae different algal diets. Newly hatched larvae of S. kraussii were fed four different marine microalgae species, singly and in various mixtures. The best growth was observed when fed C. vulgaris, N. oculata as a single species and mixed-algal diet during day 15 after fertilization. Mortality was low for larvae (max. 5%); survival rate more than 95%. These results suggest that S. kraussii larvae have the capacity to feed using alternative sources of energy, and food size and quality can affect their growth and sustainability.

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Larval development and metamorphic behaviour of the subtropical spionid polychaete Pseudopolydora vexillosa

Cited by 9 publications

References 38 publications

Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa

Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa

Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa

Influence of Different Algal Diets on Larval Growth Rates in the Marine Serpulidae Polychaete Worm Spirobranchus kraussii

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