Laryngoscopy findings and histological results in a rabbit gastroesophageal reflux model

Abstract

The role of lower esophageal sphincter (LES) in laryngopharyngeal reflux is controversial. In this study, we used an animal model to investigate the association between LES dysfunction and reflux laryngitis. Twelve healthy New Zealand albino rabbits (2.5-3.5 kg) were utilized in this study. The animals were divided into two groups. Eight rabbits underwent total cardiomyectomy to induce reflux, and the remaining four rabbits underwent a control sham operation. A laryngoscopy and a 24-hour intra-esophageal pH-metry were performed prior to surgery and again 2 and 8 weeks postsurgery. After the final laryngoscopy, all animals were sacrificed to obtain histological results. Total cardiomyectomy significantly increased the reflux index, the duration of the longest reflux episode and the total number of episodes that occurred in 24 h postsurgery. No significant difference was observed in the reflux finding score (RFS) between preoperative and 2-week postoperative rabbits (P = 0.11). But there was a statistically significant change in the RFS before and 8 weeks after the induction of reflux from 4.6 ± 0.9 to 8.3 ± 3.6 (P = 0.02). Submucous gland hyperplasia and inflammation were significantly increased in the reflux group compared to the control group. The results of this study suggest that chronic lower esophageal sphincter dysfunction is associated with reflux laryngitis in rabbits.

Biochemical basis of vocal fold mobilization after microflap surgery in a rabbit model

“…However, in microflap procedures involving more extensive surgical dissection with a larger residual mucosal gap, sound clinical judgment should be appropriately used to guide postoperative voice use recommendations beyond the 3‐day minimum discovered in the present study. Additionally, although laryngopharyngeal reflux (LPR) was not a variable controlled for in the present study, the role of LPR on the healing microflap and the importance of controlling for objectively documented sources of laryngeal irritation during the acute stages of wound healing should not be discounted . LPR, inhaled pathogens, and other sources of laryngeal irritation have the potential to exacerbate the wound healing response and result in poor voice outcomes.…”

“…Additionally, although laryngopharyngeal reflux (LPR) was not a variable controlled for in the present study, the role of LPR on the healing microflap and the importance of controlling for objectively documented sources of laryngeal irritation during the acute stages of wound healing should not be discounted. 24 LPR, inhaled pathogens, and other sources of laryngeal irritation have the potential to exacerbate the wound healing response and result in poor voice outcomes.…”

Expression of claudin-3 in the esophagus and larynx of rat reflux model

“…Secondly, there was no laryngoscopic evaluation of laryngeal appearance in the rat model. In our previous research, we evaluated the laryngeal appearance in a rabbit reflux model [21]. However, the structural differences between the larynxes of rabbits and human beings limit the evaluation.…”

The role of Glut-1 and H+/K+-ATPase expression in hyperplasia of mice laryngeal epithelium induced by pepsin

“…Several GERD models are available, but few LPRD models. Hu et al established a rabbit reflux model employing total cardiomyectomy [19]. The limitations include a difficult operation, the need for postoperative care, and animal morbidity [19,20].…”