Laser-assisted deposition of bacteriorhodhopsin assemblies

Agarwal, Gunjan; Phadke, Ratna S.

doi:10.1088/0957-4484/10/3/318

Cited by 10 publications

(7 citation statements)

References 18 publications

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“…They showed that the constituent molecules in the deposited material displayed characteristic native properties and the assemblies retained their morphological identity. In a following publication, the same authors reported the growth of supramolecular assemblies consisting of the photosensitive protein bacteriorhodhopsin (bR) embedded in the lipid l -α-phosphatidylcholine distearoyl (DSPC) by PLD . Again the authors found that the structural and functional properties of the bR+DSPC assemblies were preserved by PLD.…”

Section: 34 Pld Of Biomaterialsmentioning

confidence: 98%

Laser Deposition of Polymer and Biomaterial Films

et al. 2003

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Section: 34 Pld Of Biomaterialsmentioning

confidence: 98%

Laser Deposition of Polymer and Biomaterial Films

et al. 2003

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“…Phadke and Agarwal used a variation of this technique to form films of bacteriorhodhopsin and glucose oxidase. 35,36 To reduce the laser interaction with the protein, a composite target was formed containing the protein in a surfactant matrix. The surfactant was found to protect the biomaterial, and films of active protein were formed.…”

Section: Introductionmentioning

confidence: 99%

“…The infrared spectrum of the deposited material was nearly identical to the bulk, pressed target material, but a mass distribution of the deposited material was not reported. Phadke and Agarwal used a variation of this technique to form films of bacteriorhodhopsin and glucose oxidase. , To reduce the laser interaction with the protein, a composite target was formed containing the protein in a surfactant matrix. The surfactant was found to protect the biomaterial, and films of active protein were formed.…”

Section: Introductionmentioning

confidence: 99%

Novel Laser-Based Deposition of Active Protein Thin Films

et al. 2001

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This paper reports the deposition of active protein thin films by a novel laser-based approach termed matrix-assisted pulsed laser evaporation (MAPLE). We have deposited uniform 10 nm to nearly 1 μm thin films of insulin and horseradish peroxidase (HRP). We performed several experiments to characterize the chemical integrity of the deposited films. Matrix assisted laser desorption/ionization and liquid chromatography/electrospray ionization mass spectrometry experiments performed on MAPLE-deposited insulin films indicate that the laser−material interaction involved in this deposition technique does not modify the protein's mass. Fourier transform infrared spectroscopy experiments show that the chemical functionality and secondary structure of MAPLE-deposited HRP are nearly identical to those of the native protein. We also find that deposited HRP films retain their ability to catalyze the reduction of 3,3‘-diaminobenzidine (DAB), suggesting that the active site of transferred proteins is unaffected by the MAPLE process. We also produced patterns and multilayers with feature sizes from 20 to 250 μm by depositing different biomaterials through a shadow mask. Patterns of physisorbed HRP were then protected from dissolution in an aqueous environment by a semipermeable polymer overlayer that was deposited in situ using pulsed laser deposition. This polymer membrane protects the protein pattern when it is exposed to DAB solution and enables the optical observation of HRP activity for spots as small as 2000 μm2. These results demonstrate that MAPLE is a preferred technique for depositing active biomolecules for applications ranging from microfluidic sensor devices to gene and protein recognition microarrays.

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“…1), which allows to grow films of inorganic [22,23] as well as organic materials [24][25][26]. It has been proven that this technique is suitable for deposition of thin films of biomaterials such as DNA [27], enzymes [28] and proteins [29,30]. The femtosecond lasers due to their advantages in material processing, such as reduced thermal effects, target damage and lower ablation threshold, can be used for the transfer of biological materials [31].…”

Section: Laser Printing Of Biomaterialsmentioning

confidence: 99%