Laser-based micro/nanoengineering for biological applications

Stratakis, Emmanuel; Ranella, Anthi; Farsari, Maria; Fotakis, C.

doi:10.1016/j.pquantelec.2009.06.001

Cited by 100 publications

(61 citation statements)

References 125 publications

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“…(12)(13)(14)(15)(16)(17) Pulse laser ablation is an attractive approach for topographically and chemically modifying surfaces. Polymer cohesion energy produced by a UV nanosecond pulse laser breaks predominantly inter-chain bonds.…”

Section: Introductionmentioning

confidence: 99%

“…(12,13) However, the precise mechanism underlying laser ablation of poly(methyl methacrylate) (PMMA) remains to be elucidated due to its complexity; it cannot currently be described by a single photochemical or mathematical model. Nonetheless, various laser ablation technologies based on surface modification have been proposed for protein immobilization (18,19) and wettability modification, (14,15) suggesting that surfaces can be modified by laser ablation for antibody immobilization. We previously described that UV laser irradiation of a plastic surface enhanced the affinity of the surface for antibodies.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic Sandwich ELISA Utilizing a Concave Microchannel Surface Generated by Nanosecond Laser Ablation

2015

Sensors and Materials

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A practical point-of-care sandwich enzyme-linked immunosorbent assay (ELISA) microchip remains elusive. A concave area on the undersurface of a microchannel was formed by nanosecond pulse laser ablation and its utility for a microfluidic system based on capillary-driven flow supporting such a microchip was determined. Ablation using a 193 nm wavelength, 1.76 J•cm −2 UV laser modified the surface of poly(methyl methacrylate), providing a surface amenable for antibody immobilization. A concave microchannel 10 µm deep and 260 µm in diameter allowed both successful antibody microdeposition and complete reagent replacement required for a practical ELISA microchip. Quantitative analysis of concentrations of procollagen-type I carboxyterminal propeptide (PICP) using this microchannel showed a linear relationship at PICP concentrations between 0-600 ng·ml −1 , which is adequate for clinical estimation of PICP concentrations in the blood.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic Sandwich ELISA Utilizing a Concave Microchannel Surface Generated by Nanosecond Laser Ablation

2015

Sensors and Materials

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“…An interesting approach is based on doping the host resin used in the 2PP with compounds of interest (organic dyes and bioactive agents). Such approach enables the fabrication of devices with specific characteristics for different areas of optics [8][9][10] and biology [11][12][13][14][15][16]. Furthermore, the manufacture of components using 2PP can be carried out in stages, allowing the fabrication of microdevices with multi-doping components [17].…”

Section: Introductionmentioning

confidence: 99%

Direct laser writing by two-photon polymerization as a tool for developing microenvironments for evaluation of bacterial growth

Otuka

Corrêa

Fontana

et al. 2014

Materials Science and Engineering: C

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“…Also these adhesive microdomains can lose their characteristics over time because cell secretions change the areas of chemical modification as well as the surface itself [21]. Other techniques like photolytography [1,22], laser printing [22], and direct laser writing [23] have several processing steps or require long processing times to pattern large area samples.…”

Section: Introductionmentioning

confidence: 99%

Contactless Laser-Assisted Patterning of Surfaces for Bio-Adhesive Microarrays

Perez-Hernandez¹,

Paumer²,

Pompe³

et al. 2012

Biointerphases

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Micropatterned surfaces with cell adhesive areas, delimited by protein repellent microstructures, are in high demand for its potential use as relevant biological assays. This is not only because such surfaces allow directing cell growth in a spatially localized and restricted manner, but also because they can be used to elucidate basic cell growth and orientation mechanisms. Here, it is presented a laser-assisted micropatterning technique to fabricate large area microstructures of poly (ethylene glycol) hydrogel onto a cell adhesive surface: a biofunctional maleic anhydride copolymer. By varying photoinitiator, laser intensity, copolymer as well as the hydrogel layer thickness, the optimum conditions to produce high quality features were found. The suitability of these micropatterned substrates for bioassay applications was proved by cell adhesion studies. The introduced procedure could be used to prepare a broad range of microarrays for certain bioanalytical approaches and to create different types of biofunctional surfaces.

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Laser-based micro/nanoengineering for biological applications

Cited by 100 publications

References 125 publications

Microfluidic Sandwich ELISA Utilizing a Concave Microchannel Surface Generated by Nanosecond Laser Ablation

Microfluidic Sandwich ELISA Utilizing a Concave Microchannel Surface Generated by Nanosecond Laser Ablation

Direct laser writing by two-photon polymerization as a tool for developing microenvironments for evaluation of bacterial growth

Contactless Laser-Assisted Patterning of Surfaces for Bio-Adhesive Microarrays

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