Laser Raman detection of platelet as a non-invasive approach for early and differential diagnosis of Alzheimer's disease

Abstract

Early and differential diagnosis of Alzheimer's disease (AD) is a problem that puzzled many doctors. Reliable markers in easy-assembling samples are of considerable clinical diagnostic value. In this work, laser Raman spectroscopy (LRS) was developed a new method that potentially allows early and differential diagnosis of AD from the platelet sample. Raman spectra of platelets isolated from different ages of AD transgenic mice and non-transgenic controls were collected and analyzed. Multilayer perceptron networks (MLP) classification method was used to classify spectra and establish the diagnostic models. For differential diagnosis, spectra of platelets isolated from AD, Parkinson's disease (PD) and vascular dementia (VD) mice were also discriminated. Two notable spectral differences at 740 and 1654 cm −1 were revealed in the mean spectrum of platelets isolated from AD transgenic mice and the controls. MLP displayed a powerful ability in the classifying of early, advanced AD and the control group, and in differential diagnosis of PD and advanced AD, as well as VD and advanced AD. The results suggest that platelet detecting by LRS coupled with MLP analysis appears to be an easy and accurate method for early and differential diagnosis of AD. This technique could be rapidly promoted from laboratory to the hospital.

A laser Raman tweezers study of eryptosis

“…Spectroscopy techniques such as Raman spectroscopy may provide a simpler way to analyse the cellular changes without external labelling . Raman spectroscopy measurements have been used for detection and monitoring of different blood‐related disorders . Raman spectroscopy combined with optical tweezers—a technique for trapping micron‐sized particles—provides a sophisticated way to monitor the biomolecules inside live cells .…”

Dengue blood analysis by Raman spectroscopy

“…Raman spectros copy (RS) is a powerful technique for optical diagnos tics of biological tissues. It was used for the detection of diseased tissues such as cervical pre cancer, viability transitions of umbilical cord mesenchymal of stem cells, rapid blood analysis, measurement of biological analytes, ischemia damaging action on blood serum, optical trapping of mononuclear cells, molecular abnormalities in benign and malignant tissues, non invasive approach for early and differential diagnosis of Alzheimer's disease using platelets and in invio der matology [2][3][4][5][6][7][8][9]. It is a spectroscopic technique used to study vibrational, rotational, and other low fre quency modes in a system and the mechanism of bind ing of human serum albumin to molecular probe fluo rescein [10].…”

Analysis of platelets in hypertensive and normotensive individuals using Raman and Fourier transform infrared‐attenuated total reflectance spectroscopies

“…Thus, both techniques can be considered complementary. Raman spectroscopy has been implemented successfully to study platelet behavior, conformational changes in the platelet‐specific integrin α II β 3 , and possible biomarkers in platelets that may be related to the development of Alzheimer's disease . Meanwhile, FTIR‐ATR has been used to characterize the adhesion of platelets onto different surfaces and biochemical changes induced by hemodialysis in plasma …”