Latex Allergy within a Cohort of Not-at-Risk Subjects with Respiratory Symptoms: Prevalence of Latex Sensitization and Assessment of Diagnostic Tools

Mari, Adriano; Scala, Enrico; D’Ambrosio, Claudio; Breiteneder, Heimo; Wagner, Stefan

doi:10.1159/000099080

Cited by 26 publications

(19 citation statements)

References 69 publications

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“…Thirteen latex-proteins have been identified and characterized [1]. Hev b5 and Hev b6 play a major role in sensitized healthcare-workers [2,3] and in patients with respiratory symptoms [4]. Healthcare-workers seem having persistent skin-test-reactivity despite avoiding latex-contact [5].…”

mentioning

confidence: 99%

Microarray‐based component‐resolved diagnosis of latex allergy: isolated IgE‐mediated sensitization to latexprofilin Hev b8 may act as confounder

Schuler

Schmid‐Grendelmeier

Harr

2013

Clinical & Translational All

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Immediate type allergy to latex is still a widespread problem. Latex-allergic patients undergoing diagnostic and operative medical procedures are at risk of potentially life-threatening reactions. Accurate diagnostic methods are therefore crucial. The aim of this retrospective study was to discriminate between sensitization and relevant allergy to latex based on an easy and suitable diagnostic approach. In 14 patients with clinical symptoms and 27 controls, latex skin prick tests (SPT), IgE against latex (CAP) and serological component resolved specific latex-allergen determination (Hev b1, b3, b5, b6, b7, b8, b9, b10, b11) based on ImmunoCAP ISAC were performed. SPT correlated very well with clinically manifest latex-allergy demonstrating a high specificity (95%) (and a low sensitivity). However, CAP levels to crude latex could not safely discriminate between purely sensitized and latex-allergic patients. The majority of patients mono-sensitized to the latex profilin Hev b8 did not suffer from any relevant symptoms upon contact with latex. However, in two patients with latex-allergy diagnosed by elevated specific IgE only sensitized against Hev b8, additional sensitization to carbohydrate cross-reactive determinants (CCD) was found. In the case of positive serum IgE against latex and negative SPT, component-resolved diagnosis including IgE against specific latex-proteins, specially Hev b8, and carbohydrate cross-reactive determinants (CCD) is a useful tool to discriminate between latex-sensitization and latex-allergy.

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confidence: 99%

Microarray‐based component‐resolved diagnosis of latex allergy: isolated IgE‐mediated sensitization to latexprofilin Hev b8 may act as confounder

Schuler

Schmid‐Grendelmeier

Harr

2013

Clinical & Translational All

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“…Among the 15 molecularly characterized NRL Hev b allergens, Hev b 5 and b 6 seem to play a major role in sensitizing healthcare workers [30,42] and patients with respiratory symptoms [43]. Moreover, spina bifida patients typically show sensitization to Hev b 1 and b 3, but also to Hev b 5, b 6 [44] and b 7 [45], even if to a lesser extent.…”

Section: Discussionmentioning

confidence: 99%

Molecular profile of sensitization in subjects with short occupational exposure to latex

Lamberti¹,

Buonanno²,

Ritonnaro³

et al. 2015

Int J Occup Med Environ Health

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Objectives:We examined the prevalence of latex allergy in subjects with occupational exposure to latex allergens for less than 5 years, determining the disease spectrum in symptomatic workers. We identified the most frequent molecular allergens by Immuno-CAP (ICAP), correlating the findings with skin prick test (SPT) results. Material and Methods: Seven hundred twenty-three healthcare students using latex gloves on a regular basis were invited to participate in a baseline questionnaire screening. An ICAP serum test was performed only when a possible latex allergy was indicated by the questionnaire. Results: The total number of participants responding to the baseline survey was 619. Glove-related symptoms were indicated by 4% (N = 25) of the students. The most common symptom was contact dermatitis (N = 18, 72%). In 12 subjects, ICAP revealed a real sensitization to latex, with a recombinant latex allergen profile showing a high frequency for rHev b 6.01 specific immunoglobulin E (sIgE) (N = 9, 67%). In these individuals, skin symptoms were more prevalent than other types (88%). Conclusions: The combined positivity for rHev b 6.01, rHev 8 and rHev b 5 determined by ICAP identified 92% of latex-allergic subjects with short-term exposure to latex.

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“…Nevertheless, the clinical relevance of an allergenic molecule-based diagnosis has become more and more evident by the use of small panels of allergenic molecules via established in vitro systems [18,19]. An increasingly comprehensive panel of natural and recombinant allergens is currently available on singleplex IgE detection systems [4••].…”

Section: Allergens: Extracts or Moleculesmentioning

confidence: 99%

Microarrayed Allergen Molecules for the Diagnosis of Allergic Diseases

Mari

Alessandri

Bernardi

et al. 2010

Curr Allergy Asthma Rep

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IgE-mediated allergic diseases are among the most prevalent diseases worldwide. The use of extracts in the skin test and the additional use of IgE testing still represent the current basis for the diagnostic work-up. During the past 30 years, knowledge of the molecular structure of allergens has increased dramatically, and the characterization and production of allergenic molecules, as natural purified compounds or recombinant products, is allowing us to approach the allergy diagnostic work-up differently. Much of this is based on the adoption of microtechnology since the first release of a biochip for IgE detection. Its use has prompted the development of new concepts linked to the diagnosis of allergic diseases. This review describes the background of allergy diagnosis and the tools currently used for specific IgE detection. It gives insight into the most recent advancement in the field of biotechnology leading to allergenic molecule availability, microtechnology leading to the routine use of protein biochips for IgE detection, and how they should be combined with information technology.

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Latex Allergy within a Cohort of Not-at-Risk Subjects with Respiratory Symptoms: Prevalence of Latex Sensitization and Assessment of Diagnostic Tools

Cited by 26 publications

References 69 publications

Microarray‐based component‐resolved diagnosis of latex allergy: isolated IgE‐mediated sensitization to latexprofilin Hev b8 may act as confounder

Microarray‐based component‐resolved diagnosis of latex allergy: isolated IgE‐mediated sensitization to latexprofilin Hev b8 may act as confounder

Molecular profile of sensitization in subjects with short occupational exposure to latex

Microarrayed Allergen Molecules for the Diagnosis of Allergic Diseases

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