Layer‐by‐layer self‐assembly polyelectrolytes loaded with cyclic adenosine monophosphate enhances the osteo/odontogenic differentiation of stem cells from apical papilla

Abstract

Cyclic adenosine monophosphate (cAMP) is a second messenger involved in the dental regeneration. However, efficient long-lasting delivery of cAMP that is sufficient to mimic the in vivo microenvironment remains a major challenge. Here, cAMP was loaded in stem cells from apical papilla (SCAPs) using layer-by-layer self-assembly with gelatin and alginate polyelectrolytes (LBL-cAMP-SCAPs). LBL-cAMP-SCAPs expressed cAMP and increased

Cyclic Adenosine Monophosphate-Enhanced Calvarial Regeneration by Bone Marrow-Derived Mesenchymal Stem Cells on a Hydroxyapatite/Gelatin Scaffold

“…cAMP, a physiologically important secondary messenger, plays a significant role in transmitting intracellular signals by conveying the cAMP-dependent pathway. cAMP is responsible for inducing osteogenic differentiation by enhancing the osteogenic potential of stem cells from apical papilla and human mesenchymal stem cells (MSCs). , Such a mechanism is able to promote the osteogenic effects of MSCs that were further correlated with the cAMP/protein kinase A (PKA) signaling pathway . Activation of cAMP/PKA also promoted osteoblast cell adhesion on biodegradable scaffolds .…”