Objective To investigate the effect of silver diamine fluoride (SDF) and potassium iodide (KI) treatment on dentine discolouration and the shear bond strength (SBS) of glass ionomer cements (GICs) to artificial caries‐affected dentine. Materials and methods Dentine slices from human molars were demineralised to mimic caries‐affected dentine. They were randomly allocated for treatment (n = 20 per treatment) with SDF + KI, SDF (positive control) or water (negative control). All slices were immersed in the artificial saliva for 24 hours after treatments. The colour of the treated surfaces was assessed using the CIELAB system. Lightness values were measured. Total colour change (∆E) was calculated using water as the reference group, and was visible to the naked eyes if ∆E > 3.7. All dentine slices were bonded with GICs. The SBS was assessed using a universal testing machine. Colour parameters and the SBS were analysed using a one‐way ANOVA test. Results The slices treated with SDF + KI had a higher lightness value those slices treated with water, whereas those treated with SDF presented a lower lightness value compared with those treated with water. The treatment with SDF + KI did not introduce any adverse colour effect to demineralised dentine (∆E = 14.4), whereas the application of SDF alone caused significant staining (∆E = 24.6). The SBS values (mean ± SD) after treatment with SDF + KI, SDF and water were 3.0 ± 1.4 MPa, 2.3 ± 0.9 MPa and 2.6 ± 1.1 MPa, respectively (P = 0.217). Conclusion The immediate application of KI solution after SDF treatment does not negatively affect adhesion of GICs to artificial caries‐affected dentine. Moreover, KI treatment can reduce discolouration of demineralised dentine caused by SDF.

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As soon as saliva contacts the teeth surface, salivary proteins adhere to the tooth surface to form acquired salivary pellicle. The formation of this acquired salivary pellicle is a dynamic and selective process of macromolecular adsorption and desorption. Although acquired salivary pellicle contains proteins and peptides, it also contains lipids, and other macro-molecules, all of which contribute to its protective properties. Acquired salivary pellicle is related to the development of common oral diseases, such as erosion, dental caries, and periodontal disease. Acquired salivary pellicle acts as a natural barrier to prevent a tooth's surface from making direct contact with acids and to protect it from erosive demineralization. It contributes to the control of dental erosion by modulating calcium and phosphate concentrations on the tooth surface. It also influences the initial colonizer of oral biofilm and affects the transportation pathway of the acidic products of cariogenic bacteria, which affects the development of dental caries. In addition, it influences periodontal disease by acting on the colonization of periodontal pathogens. This paper's aim is to provide an overview of the acquired salivary pellicle, highlighting its composition, structure, function, role in common oral diseases, and modification for the prevention of oral diseases.

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