Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells

Abstract

Gene and drug delivery systems need crucial update in the issue of nanocarriers. Layered double hydroxides (LDHs) are known as biocompatible inorganic lamellar nanomaterials with versatile properties. In the present study, Zn/Al-LDH nanoparticle was synthesized and characterized by FTIR, XRD, SEM, TEM and Zeta potential tests and then intercalated with valproate and methyldopa by co-precipitation and ion exchange methods. These nanocarriers were applied as high activity nanolayers-based delivery systems. On the other hand, Zn/Al-LDH þ plasmid/gene (pCEP4/Cdk9) evaluated on C2C12 myoblast cells. Cooperation loading indicated high efficiency of sorting and release of drugs. Additionally, the Real-Time PCR and Western blotting results for plasmid-gene (pCEP4/Cdk9) delivery showed that Zn/Al-LDH nanoparticles can be used as an effective carrier in cellular uptake and release of genes for gene therapy. Easy and cost-effective production of Zn/Al-LDH nanoparticles proposed them as potential alternatives for the traditional routs of drug/gene delivery.

Layered Double Hydroxides: A Toolbox for Chemistry and Biology

“…The authors delaminated the LDH nanoparticles by intercalating lactate into the layers of LDH The results from cellular internalization experiments demonstrated a strong inhibition of the proliferation of HL-60 cancer cells exposed to As-myc-LDH hybrids, reaching 65% growth inhibition compared with untreated cells. In a more recent report, ZnAl LDH nanoparticles were loaded with the plasmid pCEP4 to permit the expression of the Cdk9 gene in C2C12 myoblasts cells, as confirmed by PCR and Western blotting results [187]. They were also loaded with valproate and methyldopa drugs, allowing for sustained pH-triggered drug delivery.…”

Overcoming multidrug resistance in cancer: Recent progress in nanotechnology and new horizons

“…Nanomedicine, innovative and alternative use of nanotechnology in medicine, opens new windows in the reversing MDR due to its unique biological and physicochemical characteristics. Some features, which make using of nanomedicine suitable and promising in overcoming the resistant phenotype of tumor cells, include small size of the drug carrier, passive or active targeting to cancer cells, improved pharmacokinetics and biodistribution, reduced side effects of the formulated drug, intrinsic inhibitory activity to MDR efflux pump proteins, and feasibility for delivery of multiple therapeutic agents in a single formulation . Multiple mechanisms of MDR provide a diverse avenue for therapeutic interventions to reverse MDR.…”

“…Some features, which make using of nanomedicine suitable and promising in overcoming the resistant phenotype of tumor cells, include small size of the drug carrier, passive or active targeting to cancer cells, improved pharmacokinetics and biodistribution, reduced side effects of the formulated drug, intrinsic inhibitory activity to MDR efflux pump proteins, and feasibility for delivery of multiple therapeutic agents in a single formulation. 4 Multiple mechanisms of MDR provide a diverse avenue for therapeutic interventions to reverse MDR. In this review, we provide an overview of conventional strategies as well as novel methods for reversing MDR in cancer cells.…”

Synthesis and Application of Magnetic@Layered Double Hydroxide Multicore-Shell Nanostructure as a Novel Anti-Inflammatory Drugs Nanocarrier

“…Inorganic x+ [A n− ] x/n .mH 2 O, where M 2+ and M 3+ are respectively di-and trivalent metal cations, and A is n-valent interlayer guest anion. The primary constituents of LDHs are the charged layers that provide diverse chemical compounds with versatile usability, for example, biocompatibility, adsorption, intercalation and ion exchange [8][9][10] . These are the basis of LDHs diverse technology applications in a variety of elds including medicine, polymer industries, electrochemistry, food, catalysis, drug delivery separation and more.…”

“…In opposition to the previous drug delivery systems, which show low circulation stability, poor bioavailability, and drug degradation; LDH as exquisite drug nano-carriers are comparatively economical with little toxicity for the cells. Moreover, easy of production, and large capacity for affective drug transportation; make them as an ideal nano-carreirs [9] . Additionally, the achievements of previous studies in this scope have encouraged us to used LDH nanostructures, as a suitable drug carrier.…”