LC–MS Determination of Gabapentin from Human Plasma

Ojha, Ashwini; Rathod, Rajeshwari; Patel, Chaula; Padh, Harish

doi:10.1365/s10337-007-0423-3

Cited by 12 publications

(4 citation statements)

References 28 publications

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“…These fragment ions were also present in the LE function and had been reported by other authors for determination of gabapentin by QqQ. [43][44][45] Elemental composition for these two fragments was calculated based on their accurate masses obtaining errors of 0.7 and 0.2 mDa, respectively.…”

Section: Application To Samplesmentioning

confidence: 54%

Target and non-target screening strategies for organic contaminants, residues and illicit substances in food, environmental and human biological samples by UHPLC-QTOF-MS

et al. 2012

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In this paper, we illustrate the potential of ultra-high performance liquid chromatography (UHPLC) coupled with hybrid quadrupole time-of-flight mass spectrometry (QTOF MS) for large scale screening of organic contaminants in different types of samples. Thanks to the full-spectrum acquisition at satisfactory sensitivity, it is feasible to apply both (post)-target and non-target approaches for the rapid qualitative screening of organic pollutants in food, biological and environmental samples. Different strategies have been applied and compared in this work. The first approach consists of target screening based on automatically extracting the exact analyte masses with a narrow mass window (AE10 mDa). The selection of analytes can be made after MS acquisition as non-specific analyte information is required when injecting the samples. The second, non-targeted approach, consists of a first component detection step followed by the search of the detected components in home-made spectral libraries. In this work, two types of libraries have been evaluated: a theoretical database, including the molecular formula of a large number of pollutants ($1000), and an empirical mass spectra library which includes a lower number of compounds for which reference standards were available. In all cases the confidence of the identification process was excellent, thanks to the value of information given in QTOF MS E acquisition mode (i.e. simultaneous acquisition of low and high energy TOF MS spectra in a unique run). Both, target and non-target approaches, are complementary and both have advantages and drawbacks. Their application to different types of samples has allowed the detection of diverse organic compounds, for example the mycotoxin fumonisin B1 in food samples, cocaine and several metabolites in human urine, as well as several pesticides, antibiotics and drugs of abuse in urban wastewater.

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Section: Application To Samplesmentioning

confidence: 54%

Target and non-target screening strategies for organic contaminants, residues and illicit substances in food, environmental and human biological samples by UHPLC-QTOF-MS

et al. 2012

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“…1 The several analytical methods have been developed for the determination of GB in biological fluids. Among the methods are gas chromatography (GC), 2,3 gas chromatography-mass spectrometry (GC-MS), [4][5][6] high-performance liquid chromatography (HPLC), [7][8][9][10][11][12][13][14][15][16][17][18][19][20] liquid chromatography-mass spectrometry (LC-MS), [21][22][23][24] capillary electrophoresis (CE) 25 and spectrofluorimetry. 26 A few analytical methods have been reported for the determination of GB in pharmaceutical preparations in the literature including spectrofluorimery and spectrophotometry, 27,28 flow analysis, 29 CE 30 and HPLC.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Spectrofluorimetric Method of Analysis for Gabapentin in Pure and Pharmaceutical Preparations

Ulu

Kel

2011

Chin. J. Chem.

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Highly sensitive spectrofluorimetric method was developed and validated for the determination of gabapentin in pure and pharmaceutical preparations. The method was based on nucleophilic substitution reaction of gabapentin with 4-fluoro-7-nitrobenzofurazan (NBD-F) in an alkaline medium (pH 9.5) to form a highly fluorescent derivative that was measured at 521 nm after excitation at 458 nm. The factors affecting the reaction was carefully studied and optimized. The method was successfully validated for linearity, limit of detection, limit of quantification, accuracy, precision, robustness and specificity. Under the optimized conditions, linear relationship with good correlation coefficient (0.9998) was found between the relative fluorescence intensity and gabapentin concentration in the range of 10-100 ng•mL -1 . The limit of detection and limit of quantification were 0.43 and 1.30 ng•mL -1 , respectively.The proposed method was successfully applied to the determination of gabapentin in its pharmaceutical capsules.The results obtained by the proposed method were comparable with those obtained by the official method. Statistical comparison by t-and F-tests revealed that there was no significant difference between the results of the two methods with respect to mean values and standard deviations at the 95% confidence level.

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“…Gabapentin (1-(aminomethyl)cyclo-hexaneacetic acid) is a structural analogue of g-aminobutyric acid (GABA) (Scheme 1) and its action is attributed to the irreversible inhibition of the enzyme GABA-transaminase, thus preventing the physiological degradation of GABA in the brain; a secondary mechanism of a blockade for GABA uptake is also suggested 1 , it is an antiepileptic effective in the treatment of partial seizures with or without secondary generalization and is used as adjunctive therapy in patients unresponsive to or intolerant of standard antiepileptic drugs 2 . A survey of the literature reveals that there were few reported methods for the determination of gabapentin using spectrophotometric technique [3][4][5] , spectrofluorimetry 6,7 , capillary electrophoresis 8 , LC-MS 9 and HPLC 10,11 . Scheme 1.…”

Section: Introductionmentioning

confidence: 99%

Spectrscopic and Conductometric Analysis of Gabapentin

Anis

Hosny

Abdellatef

et al. 2011

Journal of Chemistry

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Four simple, sensitive and reproducible methods were developed for the determination of gabapentin (GPT) in pure form and in pharmaceutical preparations. Methods A and B are based on the reaction of cupric chloride with gabapentin to form stable complex, which could be measured spectrophotometrically at λmax246 nm (method A) or by using conductometric technique (method B). While method C and D depends on the formation of ion pair complex between the studied drug and bromothymol blue, bromocresol green respectively this was extractable with methylene chloride. The concentration ranges were 40-95 μg mL-1, 1-15 mg, 100-800 and 10-150 μg mL-1for methods A, B, C and D respectively .The optimization of various experimental conditions were described .The results obtained showed good recoveries, Ringbom optimum concentration ranges were calculated, in addition to molar absorptivity and sandell’s sensitivity, detection and quantification limits. The methods were successfully applied to the determination of GPT in bulk and pharmaceutical preparations. The results were favorably comparable with the official method. The molar combining ratio for methods (A-B) was found to be (2:1) (drug: reagent) while for method (C-D) it was found to be (1:1).

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LC–MS Determination of Gabapentin from Human Plasma

Cited by 12 publications

References 28 publications

Target and non-target screening strategies for organic contaminants, residues and illicit substances in food, environmental and human biological samples by UHPLC-QTOF-MS

Target and non-target screening strategies for organic contaminants, residues and illicit substances in food, environmental and human biological samples by UHPLC-QTOF-MS

Sensitive Spectrofluorimetric Method of Analysis for Gabapentin in Pure and Pharmaceutical Preparations

Spectrscopic and Conductometric Analysis of Gabapentin

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