LC–MS–MS Separation and Simultaneous Determination of Tolterodine and its Active Metabolite, 5-Hydroxymethyl Tolterodine in Human Plasma

Electrochemical Oxidation of Tolterodine

“…The fragmentation spectrum of tolterodine (Figure 5a) shows fragment ions at m / z 284 (loss of a propyl group from the amine), at m / z 197 (cleavage of N ‐ethyl‐ N , N ‐diisopropylamine group) and the most intensive ion at m / z 147 (cleavage of the N , N ‐diisopropylamine group together with the unsubstituted phenyl ring and a hydrogen). The fragmentation spectrum of tolterodine corresponds to the spectrum reported in the literature 7.…”

Electrically-enhanced microextraction combined with maltodextrin-modified capillary electrophoresis for quantification of tolterodine enantiomers in biological samples

“…Common methods that have been used for routine analysis of TOL enantiomers were chromatographic and immunoassay techniques [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. In all these methods, the problem of sample pretreatment 8.…”

“…TOL enantiomers have a narrow therapeutic/toxic index and the degree of plasma protein binding of TOL is approximately 96% [12]. In order to ensure correct determination of each enantiomer of TOL, the drug-protein interactions have to be eliminated.…”

“…Determination of TOL has already been carried out using chromatographic methods such as gas chromatography-mass spectrometry (GC-MS) [7], capillary electrophoresis (CE) [8,9], high performance thin layer chromatography (HPTLC) [10,11] and high performance liquid chromatography (HPLC) with different detectors including tandem mass spectrometry (HPLC-MS-MS) [12][13][14][15], mass spectrometry (HPLC-MS) [16,17], and UV detection (HPLC-UV) [18][19][20][21][22]. But only a few published chromatographic methods are available for the determination of TOL enantiomers [8,9,18,19].…”

Determination of Tolterodine tartrate in bulk and formulation by extractive colorimetric method using Tropaeolin OOO-1

“…The methods reported in the literature for its determination either in biological matrix or in pharmaceutical formulations are ultra violet (UV) and visible spectrophotometric methods [5][6][7][8][9], High Performeance Liquid Chromatography (HPLC) [10,14], Ultra High performance liquid chromatography (UPLC) [14] and potentiometric determinations using ion selective electrodes [15]. Even though, the regular methods such as HPLC and liquid chromatography-electron spray tandem mass spectrophotmetry (LC-MS/MS) are more accurate in estimating the drug in nanogram level but they need complex sample treatment, expensive solvents and instruments, and reagents for analysis.…”