Leaf senescence induced by EGY1 defection was partially restored by glucose in Arabidopsis thaliana

Chen, Cuiyun; Wang, Jin; Zhao, Xin

doi:10.1186/s40529-016-0120-3

Cited by 13 publications

(14 citation statements)

References 42 publications

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“…Taken together, these results suggest that SiYGL2 may be involved in delaying the onset of leaf senescence. This is consistent with the results reported for the SiYGL2 homolog AtEGY1 in Arabidopsis ( Chen et al, 2016 ).…”

Section: Discussionsupporting

confidence: 93%

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SiYGL2 Is Involved in the Regulation of Leaf Senescence and Photosystem II Efficiency in Setaria italica (L.) P. Beauv.

et al. 2018

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A yellow-green leaf mutant was isolated from EMS-mutagenized lines of Setaria italica variety Yugu1. Map-based cloning revealed the mutant gene is a homolog of Arabidopsis thaliana AtEGY1. EGY1 (ethylene-dependent gravitropism-deficient and yellow-green 1) is an ATP-independent metalloprotease (MP) that is required for chloroplast development, photosystem protein accumulation, hypocotyl gravitropism, leaf senescence, and ABA signal response in A. thaliana. However, the function of EGY1 in monocotyledonous C4 plants has not yet been described. The siygl2 mutant is phenotypically characterized by chlorotic organs, premature senescence, and damaged PS II function. Sequence comparisons of the AtEGY1 and SiYGL2 proteins reveals the potential for SiYGL2 to encode a partially functional protein. Phenotypic characterization and gene expression analysis suggested that SiYGL2 participates in the regulation of chlorophyll content, leaf senescence progression, and PS II function. Additionally, our research will contribute to further characterization of the mechanisms regulating leaf senescence and photosynthesis in S. italica, and in C4 plants in general.

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Section: Discussionsupporting

confidence: 93%

“…These data indicated that the loss of AtEGY1 function accelerated leaf aging and decreased photosystem II (PS II) efficiency. In addition, exogenously applied glucose could rescue these mutant phenotypes ( Chen et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

SiYGL2 Is Involved in the Regulation of Leaf Senescence and Photosystem II Efficiency in Setaria italica (L.) P. Beauv.

et al. 2018

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“…Our results showed that chlorophyll contents were low in pop2-3 mutant than wild-type plant after 30 days in normal growth condition. Probably low chlorophyll content in mutant showed precocious leaf senescence [ Figure 1a], which was similar to the reported data in egy1 mutants, [27] porB1porC-1 double mutant of older seedlings, [30] and mex1 leaves of A. thaliana. [31] The chlorophyll content of GABA-T complement plant was approximately similar to wild-type plant up to 30 days [ Figure 1a].…”

Section: Discussionsupporting

confidence: 89%

“…Similar experiments were performed for the complementation of egy1 mutant to ensure the function of ethylene-dependent gravitropism-deficient and yellowgreen 1 in regulation of leaf development and senescence in A. thaliana. [27] Several other investigators also performed the same experiment to confirm the role of a respective genes such as TLP 18.3 gene, [12] ASP2 gene, [28] and CYP724A1 gene. [29] We have previously established physiological parameters for the screening of leaf senescence that based on the determination of chlorophyll content, ion leakage, and MDA content at different leaf developmental stages.…”

Section: Discussionmentioning

confidence: 99%

“…The result was as similarly found in egy1 mutant leaf of A. thaliana. [27] Determination of lipid peroxidation is another parameter to measure the leaf senescence. Our result showed that increases the level of lipid peroxidation and cell damage in pop2-3 mutant plant in an earlier stage of leaf development as compared to wild type [ Figure 1c].…”

Section: Discussionmentioning

confidence: 99%

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Ansari¹

2017

IJGP

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Aim: Gamma-aminobutyric acid (GABA) is the chief inhibitory neurotransmitter in the mammalian central nervous system, and its important role is reducing the neuronal excitability throughout the nervous system. The GABA transaminase (GABA-T) enzyme catalyzes the transamination of GABA to form succinic semialdehyde using pyruvate as amino acid acceptor in GABA shunt pathway. The Arabidopsis thaliana GABA-T gene (pollenpistil incompatibility 2 [POP2]; At3g22200) belongs to Class III pyridoxal-phosphate-dependent aminotransferase family. The full-length cDNA of the gene encodes a protein of 513 amino acids residues and located at chromosome 3. It could be demonstrated that the GABA-T is involved in the regulation of leaf senescence in A. thaliana. Materials and Methods: To ensure the role of GABA-T gene in leaf senescence, we have performed functional complementation using transformation of pPZP200GB-GABA-T construct into pop2-3 T-DNA insertion mutant (GABA-T knockout mutant) of A. thaliana (GABA-T complement plant) that the mutant plant reverts back to wild-type plant. We have measured and compared the physiological characteristics associated with senescence (chlorophyll content, ion leakage and lipid peroxidation) and quantified the GABA shunt components (GABA content, GABA-T, and glutamate decarboxylase activity) of the wild-type, pop2-3 mutant, and GABA-T complement plant of A. thaliana. Results: The result showed the low chlorophyll content, increased ion leakage, and higher level of lipid peroxidation in pop2-3 mutant than wild-type plant after 30 days in normal growth condition. GABA-T pop2-3 mutants act as signals that mediate early leaf senescence. GABA-T complement plant shared similar characteristics of senescence parameters with wild-type plants. Conclusion: These data concluded that GABA-T is involved in the regulation of leaf senescence in A. thaliana.

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iTRAQ-based quantitative proteomic analysis reveals dynamic changes during daylily flower senescence

Shi

Zou

et al. 2018

Planta

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Sugar-related metabolic biological processes and metabolic pathways as well as invertase, protease, and ribosomal proteins may be critical regulators controlling the circadian rhythm and ephemeral properties of daylily flowers. Daylily is a familiar perennial flower. The daylily flower opens at dawn and withers away at night. Flower longevity in almost all daylily varieties from opening to fading is less than 24 h. In the past decades, the physiological changes and genetic responses to senescence in daylily flowers have been reported. However, the main metabolic pathways and biological processes involved in daylily flower senescence and the proteins involved in premature senility of daylily flowers are poorly understood. Herein, we identified differences between the proteomes of four developmental stages (s1-s4) of daylily flowers using iTRAQ-based quantitative proteomic methods. A total of 445 proteins (containing at least two unique peptides) were identified, and differentially expressed proteins (upregulation ≥ 1.5 or downregulation ≤ 0.67, P value ≤ 0.05) were detected between these stages in the following numbers: 58 (s2/s1), 59 (s3/s1), 31 (s3/s2), 64 (s4/s1), 52 (s4/s2), and 29 (s4/s3). Protein functions and classifications were analyzed based on GO, KEGG, and COG, and expressive hierarchical cluster analysis and functional enrichment analysis for differentially expressed proteins were carried out. A comparison of the late stages (s3 and s4) with the early stage (s1) revealed that the sugar (hexose, monosaccharide, and glucose) metabolic process GO category was the most enriched, and sugar (galactose, pentose, starch, and sucrose) metabolism pathways constituted the most enriched KEGG category. Finally, the potential research value of invertase, protease, and ribosomal proteins for revealing the mechanism underlying the circadian rhythm and ephemeral properties of daylily flowers are discussed. These data and analyses provide new insight into the senescence mechanism of daylily flowers.

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Leaf senescence induced by EGY1 defection was partially restored by glucose in Arabidopsis thaliana

Cited by 13 publications

References 42 publications

SiYGL2 Is Involved in the Regulation of Leaf Senescence and Photosystem II Efficiency in Setaria italica (L.) P. Beauv.

SiYGL2 Is Involved in the Regulation of Leaf Senescence and Photosystem II Efficiency in Setaria italica (L.) P. Beauv.

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iTRAQ-based quantitative proteomic analysis reveals dynamic changes during daylily flower senescence

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