Leaf Total Nitrogen Concentration as an Indicator of Nitrogen Status for Plantlets and Young Plants of Eucalyptus Clones

Ferreira, Eric Victor de Oliveira; Novais, Roberto Ferreira; Médice, Bruna Maximiano; Barros, Nairam Félix de; Silva, Ivo Ribeiro

doi:10.1590/01000683rbcs20140689

Cited by 12 publications

(7 citation statements)

References 29 publications

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“…Forde and Lea (2007) suggested that at different stages of growth there may be great variation in the level of soluble amino acids, but glutamate varies less than other amino acids, particularly glutamine. For clone VM-01, the greater glutamine (Table 1) and glutamate ( Fig 3b) concentrations in plants grown at a higher N rate are in accordance with greater NH 4 + concentrations (Ferreira et al, 2015a). High NH 4 + concentration can be toxic to plants (Marschner, 2012) and thus it is incorporated into glutamine and glutamate (Ireland and Lea, 1999).…”

Section: Root Amino Acidsmentioning

confidence: 64%

“…This fact was associated with a higher total N concentration (Fig 1b) and greater protein synthesis (Ferreira et al, 2015b) and photosynthetic rate (Ferreira et al, 2015c), which resulted in an increase in the total dry matter production of the clones (Fig 1c) since the amino acids taken up are sources of C skeletons for plant metabolism (Majerowicz and Kerbauy, 2002). The smaller concentrations in leaf amino acids of the plants grown at lower N rates (0 and 0.74 mmol L -1 of NH 4 NO 3 ) were also observed through visual symptoms of generalized chlorosis, since total N concentration (Fig 1b) and chlorophyll levels were both lower (Ferreira et al, 2015a). Chlorosis is developed following the removal of the sink, causing depletion in total N, proline, arginine, and serine (Schaffer et al, 1986).…”

Section: Shoot Amino Acidsmentioning

confidence: 93%

“…Glutamine synthetase (GS) combines NH 4 + with glutamate for synthesis of glutamine (Forde and Lea, 2007). Thus, in general, the highest levels of glutamine (Fig 4b) were associated with higher levels of NH 4 + (Ferreira et al, 2015a) and glutamate (Fig 4c), but not associated with GS activity in the leaves of the clones (Ferreira et al, 2015b). Seifferta et al (2004) also found that glutamine concentrations increased significantly in oilseed rape leaves with an increase in N supply.…”

Section: Shoot Amino Acidsmentioning

confidence: 98%

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Nitrogen supply affects root and shoot amino acid composition in Eucalyptus clones

Ferreira¹,

Novais²,

Dubay³

et al. 2016

Aust J Crop Sci

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Changes in amino acid composition are frequently observed in plants under various conditions of stress, such as nutrient deficiencies. Hence, amino acids have been proposed as more sensitive indicators of N status than total N concentration. The goal of this study was to evaluate alterations in amino acid composition of young eucalyptus clones as a result of variable N supply. An experiment was carried out in a greenhouse with two Eucalyptus clones (VM-01 and I-144) and six N application rates (0, 0.74, 2.93, 4.39, 5.85, and 8 mmol L-1 of NH 4 NO 3) grown in a nutrient solution in a randomized block design. Amino acid concentrations varied greatly as a function of N supply and depended on the organ (root or leaf) and the genotype evaluated. Roots showed greater number of amino acids than leaves (17 and 14, respectively), probably because of a higher amino acids synthesis or translocation to the roots. For both clones, N deficiency induced a significant decrease in proline, arginine, and methionine concentration in roots and a decrease in tyrosine, alanine, threonine, and methionine in leaves. These decreases were also associated with lower total N concentration and total dry matter of the eucalyptus clones. In conclusion, N supply affects amino acid composition, and the amino acids listed above are likely to be more sensitive indicators of N status than total N in eucalyptus clones.

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Section: Root Amino Acidsmentioning

confidence: 64%

Section: Shoot Amino Acidsmentioning

confidence: 93%

Section: Shoot Amino Acidsmentioning

confidence: 98%

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Nitrogen supply affects root and shoot amino acid composition in Eucalyptus clones

Ferreira¹,

Novais²,

Dubay³

et al. 2016

Aust J Crop Sci

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View full text Add to dashboard Cite

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“…Immediately following photosynthesis measurements on 3 June 2014 and 10 June, 9 July, 21 July and 22 August 2015, three leaves were excised from each sapling, dried at 60°C for 72 hr, weighed and homogenized for nitrogen concentration analysis via flash combustion (Thermoquest NC 2500 autoanalyser). We use foliar nitrogen as an indicator of plant nitrogen status and proxy for plant nitrogen uptake (de Ferreira, Novais, Médice, de Barros, & Silva, ). Litterfall production and stem growth were assessed as measures of above‐ground productivity in 2013 (to determine baseline values before treatment was initiated) and in 2014 only, as herbivory damage in 2015 precluded comparable measurements due to stem regrowth and early season leaf flush failure.…”

Section: Methodsmentioning

confidence: 99%

Snow depth, soil temperature and plant–herbivore interactions mediate plant response to climate change

et al. 2018

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Northern forest ecosystems are projected to experience warmer growing seasons, as well as winters with reduced snowpack depth and duration. Reduced snowpack will expose soils to cold winter air and lead to increased frequency of freeze‐thaw cycles. The interactions between warmer soils in the growing season and colder soils in winter may have important implications for the phenology, productivity and nutrient content of forest plants. We conducted an experiment at Hubbard Brook Experimental Forest, NH, USA, to examine the effects of growing season warming, reduced depth and duration of winter snowpack, as well as increased frequency of soil freeze‐thaw cycles on sugar maple (Acer saccharum) and red maple (Acer rubrum) saplings. We examined the direct effects of soil temperatures on plant root health, timing of leaf‐out, foliar nitrogen, rates of photosynthesis and growth, as well as the indirect effects of snowpack reduction on herbivory on plant stems. A smaller winter snowpack and increased frequency of soil freeze‐thaw cycles in winter led to increased root damage and delayed leaf‐out for maple saplings. Snowpack reduction decreased rates of stem herbivory in winter, indicating that alleviation of above‐ground stem damage in winters with reduced snowpack may offset the root damage incurred from successive soil freeze‐thaw cycles in winters with low snowpack. Synthesis. By examining the response of two dominant tree species to simulated climate change in both the growing season and winter, we find that plant responses are mediated through a combination of changes in soil temperature and plant–herbivore interactions that differentially affect above‐ and below‐ground plant components. These results highlight the feedbacks between trophic levels that shape forest function and demonstrate the need for considering climate change across seasons in global change experiments to determine how forest function may change in the future.

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“…Tree leaves were collected biweekly beginning in May 2012 and continuing to the end of September 2014 to determine effects of the irrigation system and N treatment on leaf N concentration as an indicator of tree N status [32]. Data for 2015 were reported in Ayars et al [17].…”

Section: Plant Measurementsmentioning

confidence: 99%

Carbon and Nitrogen Dynamics Affected by Drip Irrigation Methods and Fertilization Practices in a Pomegranate Orchard

et al. 2019

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Knowledge of carbon (C) and nitrogen (N) dynamics under different irrigation practices in pomegranate orchards is novel and essential to develop sustainable production systems. The aim of this research was to determine the effect of high-frequency drip irrigation and different rates of N fertilizer on C and N distribution in the soil and N uptake by pomegranate fruit and leaves. The main treatments were surface drip irrigation (DI) and subsurface drip irrigation (SDI), and the sub-treatments used were three initial N rates (N1, N2, and N3). As trees grew larger, the N application rate increased. From 2013-2015, trees received the following rates of N: 62-113 (N1), 166-263 (N2), or 244-342 kg/ha (N3). Soil and leaf total C (TC) and N (TN), soil dissolved organic C (DOC), soil nitrate (NO 3 − ), and total N uptake by fruit were evaluated between 2012 and 2015. Soil samples were collected to 120 cm depth at 15 cm increments. DI resulted in higher concentrations of TN, TC, NO 3 − , and DOC in the upper 75 cm depth than SDI. The N3 treatment resulted in higher concentrations of TN, TC, NO 3 − , and DOC under both DI and SDI. Neither DI nor SDI at the N1 or N2 levels increased TN and NO 3 − concentrations at 105-120 cm soil depth, indicating reduced leaching risk using high-frequency drip irrigation. Higher N uptake by fruit was observed in SDI than in DI in 2014 and 2015, and in N2 and N3 treatments compared with N1 in 2013 and 2014. The data indicate that the application rate at 166-263 kg/ha (N2) provided sufficient N for a 4-6-year-old pomegranate orchard and that high-frequency SDI is a promising technology for achieving higher N use efficiency and minimizing leaching loss of NO 3 − and DOC.

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Leaf Total Nitrogen Concentration as an Indicator of Nitrogen Status for Plantlets and Young Plants of Eucalyptus Clones

Cited by 12 publications

References 29 publications

Nitrogen supply affects root and shoot amino acid composition in Eucalyptus clones

Nitrogen supply affects root and shoot amino acid composition in Eucalyptus clones

Snow depth, soil temperature and plant–herbivore interactions mediate plant response to climate change

Carbon and Nitrogen Dynamics Affected by Drip Irrigation Methods and Fertilization Practices in a Pomegranate Orchard

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