Lectin histochemical studies on the olfactory epithelium and vomeronasal organ in the Japanese striped snake, <i>Elaphe quadrivirgata</i>

Kondoh, Daisuke; Yamamoto, Yoshio; Nakamuta, Nobuaki; Taniguchi, Kazumi; Taniguchi, Kazuyuki

doi:10.1002/jmor.10864

Cited by 20 publications

(22 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They are considered to be the major analytic tool for the study of both soluble and cellular glycoconjugates. They are mainly specific to the terminal carbohydrates of sugar chains [7] and are extensively used for the differentiation of cells according to their glycoconjugate contents on the histological sections [9,17,19,21,22,24]. In this study, the VNO of the adult Corriedale sheep was examined by lectin histochemistry to reveal the binding patterns with the glycoconjugates in both the vomeronasal sensory and non-sensory epithelia of the sheep.…”

mentioning

confidence: 99%

Lectin Histochemical Studies on the Vomeronasal Organ of the Sheep

Ibrahim

Nakamuta

Taniguchi

et al. 2013

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The vomeronasal organ of sheep was examined using lectin histochemistry in order to compare the types and amounts of the glycoconjugates among various components of the vomeronasal sensory and non-sensory epithelia. In the vomeronasal sensory epithelium, Dolichos biflorus agglutinin (DBA) stained particular cells, located at the same level as the vomeronasal receptor cells, while the distribution, shape and number of the stained cells did not correspond to those of the vomeronasal receptor cells. Datura stramonium lectin (DSL), Concanavalin A (Con A), Phaseolus vulgaris agglutinin-E (PHA-E) and Phaseolus vulgaris agglutinin-L (PHA-L) labeled the basal cells of both vomeronasal sensory and non-sensory epithelia. While, Wheat germ agglutinin (WGA), Succinylated-wheat germ agglutinin (s-WGA), Lycopersicon esculentum lectin (LEL), Solanum tuberosum lectin (STL) and Ricinus communis agglutinin-I (RCA-120) labeled the basal cells of the sensory epithelium, and Bandeiraea simplicifolia lectin-I (BSL-I) stained the basal cells of the non-sensory epithelium, respectively. Seventeen lectins labeled the free border of both vomeronasal sensory and non-sensory epithelia, while Sophora japonica agglutinin (SJA), Jacalin and Pisum sativum agglutinin (PSA) labeled neither free border of the sensory nor that of non-sensory epithelia. The expression pattern of glycoconjugate was similar, but not identical, in the free border between the sensory and non-sensory epithelia. These results indicate that there are dissimilar features in the type and amount of glycoconjugates between the vomeronasal sensory and non-sensory epithelia, and at the same time, among the various cell types either in the vomeronasal sensory or non-sensory epithelium.

show abstract

mentioning

confidence: 99%

Lectin Histochemical Studies on the Vomeronasal Organ of the Sheep

Ibrahim

Nakamuta

Taniguchi

et al. 2013

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…As both snakes and lizards possess well-developed vomeronasal system, the present results support that glycoconjugate moieties are similar between the main olfactory bulb and the accessory olfactory bulb in the species with well-developed vomeronasal system. In addition, the lectin binding patterns in the receptor cells are reported to be similar between the main olfactory system and the vomeronasal system in the Japanese striped snake [17]. These similarities of lectin binding patterns between the main olfactory system and the a) The internal plexiform layer was stained weakly, and the mitral cell, granule cell and ependymal cell layer were stained faintly.…”

Section: Discussionmentioning

confidence: 75%

“…On the other hand, in the olfactory and vomeronasal epithelia of the Japanese striped snake, 4 lectins among these 15 lectins, SBA, PNA, ECL and PSA, stain the cell processes of the receptor cells only after sialic acid removal [18] and do not stain them before this treatment [17]. These reports suggest that several glycoconjugate moieties in receptor cells are capped by si- alic acid residues in the olfactory and vomeronasal epithelia and are not capped by sialic acid residues in the nerve and glomerular layers of the main and accessory olfactory bulbs.…”

Section: Discussionmentioning

confidence: 99%

Histological and Lectin Histochemical Studies on the Main and Accessory Olfactory Bulbs in the Japanese Striped Snake, <i>Elaphe quadrivirgata</i>

et al. 2013

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The main and accessory olfactory bulbs were examined by histological methods and lectin histochemistry in the Japanese striped snake. As the results, the histological properties are similar between the main olfactory bulb and the accessory olfactory bulb. In lectin histochemistry, 21 lectins used in this study showed similar binding patterns in the main olfactory bulb and the accessory olfactory bulb. In detail, 15 lectins stained these olfactory bulbs with similar manner, and 6 lectins did not stain them at all. Two lectins, Lycopersicon esculentum lectin (LEL) and Solanum tuberosum lectin (STL), stained the nerve and glomerular layers and did not stain any other layers in both olfactory bulbs. Four lectins, Soybean agglutinin (SBA), Vicia villosa agglutinin (VVA), Peanut agglutinin (PNA) and Phaseolus vulgaris agglutinin-L (PHA-L) stained the nerve and glomerular layers more intensely than other layers in both olfactory bulbs. In addition, VVA showed the dot-like stainings in the glomeruli of both olfactory bulbs. These findings suggest that the degree of development and the properties of glycoconjugates are similar between the main olfactory bulb and the accessory olfactory bulb in the Japanese striped snake.

show abstract

“…The differences in the properties of secretory granules contained in the supporting cells suggest the functional difference between these two epithelia. In other vertebrates, binding patterns for lectins in the cytoplasm of supporting cells are also different between the OE and the VNO [2,7,14,23]. These differences have been considered to be related to the differences in the olfactory function between the OE and the VNO.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Glycoproteins Produced by the Associated Gland in the Olfactory Organ of Lungfish

Nakamuta

Taniguchi

et al. 2013

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The olfactory organ of African lungfish, Protopterus annectens, contains two distinct sensory epithelia: the lamellar olfactory epithelium and the recess epithelium. These epithelia correspond to the olfactory epithelium and the vomeronasal organ of tetrapods, respectively. In contrast to the lamellar olfactory epithelium, which has no associated gland, the recess epithelium is equipped with associated glands. Although the glandular cells and/or the supporting cells are generally presumed to secrete proteins involved in the function of olfactory sensory epithelia, the properties of these proteins in lungfish have not been evaluated to date. In this study, we investigated the associated glands in the olfactory organ of lungfish by transmission electron microscopy and found that the glandular cells contain numerous secretory granules and secrete them from the apical membrane. In addition, we analyzed the olfactory organ by lectin histochemistry using 16 biotinylated lectins. All lectins labeled the secretory granules in the glandular cells with different staining patterns from those of the supporting cells in the lamellar olfactory epithelium or in the recess epithelium. Furthermore, lectin blotting analysis showed that multiple bands were detected by the lectins which specifically labeled the glandular epithelium of the olfactory organ. These results indicate that the secretory products of the associated glands in the recess epithelium have different properties from those of the supporting cells in the olfactory sensory epithelia and contain multiple glycoproteins with different carbohydrate moieties. KEY WORDS: electron microscopy, fish, glycoprotein, vomeronasal organ.doi: 10.1292/jvms.12-0547; J. Vet. Med. Sci. 75 (7): [887][888][889][890][891][892][893] 2013 Many vertebrates have two distinct olfactory organs, the olfactory epithelium (OE) and the vomeronasal organ (VNO) for detecting chemical substances in the environment. Unlike the OE found in all vertebrates, it has been suggested that the VNO is not present in fish, but is present only in terrestrial vertebrates [4]. However, the lungfish, phylogenetically the closest fish to tetrapods, have epithelial crypts that express VNO markers of tetrapods [10]. Recently, we have examined the olfactory organ of African lungfish, Protopterus annectens, and revealed the histological and ultrastructural characteristics of the lamellar OE and the recess epithelium, corresponding to the OE of ordinary fish and the VNO of tetrapods, respectively [19]. These two sensory epithelia are separately distributed in the olfactory organ of lungfish: the lamellar OE is located on the surface of olfactory lamellae and the recess epithelium in recesses at the base of lamellae [19].In all vertebrates, olfactory receptors detect chemical substances dissolved in the mucous fluid covering the sensory epithelium [8,20]. The OE and the VNO of terrestrial vertebrates are equipped with the associated glands: the Bowman's glands in the OE and the Jacobson's glands in the VNO [3...

show abstract

Lectin histochemical studies on the olfactory epithelium and vomeronasal organ in the Japanese striped snake, Elaphe quadrivirgata

Cited by 20 publications

References 49 publications

Lectin Histochemical Studies on the Vomeronasal Organ of the Sheep

Lectin Histochemical Studies on the Vomeronasal Organ of the Sheep

Histological and Lectin Histochemical Studies on the Main and Accessory Olfactory Bulbs in the Japanese Striped Snake, <i>Elaphe quadrivirgata</i>

Analysis of Glycoproteins Produced by the Associated Gland in the Olfactory Organ of Lungfish

Contact Info

Product

Resources

About