Lectin Histochemical Studies on the Vomeronasal Organ of the Sheep

Ibrahim, Dalia; Nakamuta, Nobuaki; Taniguchi, Kazumi; Taniguchi, Kazuyuki

doi:10.1292/jvms.12-0532

Cited by 18 publications

(22 citation statements)

References 25 publications

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“…The VNO of other cetartiodactyl species comprises several veins within the lamina propria deep in the NSE (Adams, 1986;Ibrahim et al, 2013Ibrahim et al, , 2015Kratzing, 1971;Park et al, 2014;Salazar et al, 1997Salazar et al, , 2007Salazar et al, , 2008Takigami et al, 2000;Vedin et al, 2010) like that of the giraffes shown herein. These veins are assumed to play a role in a pumping mechanism (Døving & Trotier, 1998) that uptakes odorants into the vomeronasal lumen of cetartiodactyla species.…”

Section: Discussionmentioning

confidence: 74%

“…The behavior in giraffes is generally considered as a flehmen response in which pheromones are transferred into the VNO. However, a VNO has not been established in giraffes, although the morphological and histological features of the VNO have been determined in other species of the same order such as cattle (Adams, 1986;Salazar, Quinteiro, Alemañ, & Prieto, 2008;Salazar, Quinteiro, & Cifuentes, 1997), goats (Besoluk, Eken, & Boydak, 2001;Takigami, Mori, & Ichikawa, 2000), sheep (Kratzing, 1971;Ibrahim, Nakamuta, Taniguchi, & Taniguchi, 2013;Salazar, Alemañ, Cifuentes, & Troconiz, 2007), deer (Park et al, 2014), moose (Vedin, Eriksson, & Berghard, 2010), camels (Ibrahim et al, 2015;Karimi, Mansoori Ale Hashem, Ardalani, Sadrkhanloo, & Hayatgheibi, 2014), and pigs (Salazar et al, 1997). As the vomeronasal system plays a critical role in the mating of animals that show flehmen behavior (Døving & Trotier, 1998), knowledge about the VNO in giraffes is important to comprehend the reproduction and social communication of giraffes.…”

Section: Introductionmentioning

confidence: 99%

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Histological features of the vomeronasal organ in the giraffe, Giraffa camelopardalis

Kondoh

Nakamura

Ono

et al. 2017

Microscopy Res & Technique

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The vomeronasal organ (VNO) that preferentially detects species-specific substances is diverse among animal species, and its morphological properties seem to reflect the ecological features of animals. This histological study of two female reticulated giraffes (Giraffa camelopardalis reticulata) found that the VNO is developed in giraffes. The lateral and medial regions of the vomeronasal lumen were covered with sensory and nonsensory epithelia, respectively. The vomeronasal glands were positive for periodic acid-Schiff and alcian blue (pH 2.5) stains. The VNO comprises several large veins like others in the order Cetartiodactyla, suggesting that these veins function in a pumping mechanism in this order. In addition, numerous thin-walled vessels located immediately beneath the epithelia covering the lumen entirely surrounded the vomeronasal lumen. This sponge-like structure might function as a specific secondary pump in giraffes.

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Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Histological features of the vomeronasal organ in the giraffe, Giraffa camelopardalis

Kondoh

Nakamura

Ono

et al. 2017

Microscopy Res & Technique

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“…The present histological, histochemical and ultrastructual findings show that the bear VNO possesses three types of secretory cells: PAS-positive and AB-negative secretory cells of vomeronasal glands, PAS-positive and AB-positive multicellular intraepithelial gland cells and goblet cells. The VNO of most mammals that have been investigated (Salazar et al 1996(Salazar et al , 1997Roslinski et al 2000;Ibrahim et al 2013;Lee et al 2016;Kondoh et al 2017b) possesses either PASpositive and AB-positive secretory cells of the vomeronasal glands (cow, sheep and giraffe) or PAS-positive and ABnegative secretory cells (cat, horse, lemur and vole), in addition to goblet cells. Wallaby possesses only PAS-positive and AB-negative glands, although a few cells do react to AB (Schneider et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Morphological and histological features of the vomeronasal organ in the brown bear

Tomiyasu

Kondoh

Sakamoto³

et al. 2017

Journal of Anatomy

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The vomeronasal organ (VNO) is a peripheral receptor structure that is involved in reproductive behavior and is part of the vomeronasal system. Male bears exhibit flehmen behavior that is regarded as the uptake of pheromones into the VNO to detect estrus in females. However, the morphological and histological features of the VNO in bears have not been comprehensively studied. The present study investigated the properties and degree of development of the VNO of the brown bear by histological, histochemical and ultrastructural methods. The VNO of bears was located at the same position as that of many other mammals, and it opened to the mouth like the VNO of most carnivores. The shape of the vomeronasal cartilages and the histological features of the sensory epithelium in the bear VNO were essentially similar to those of dogs. Receptor cells in the VNO of the bear possessed both cilia and microvilli like those of dogs. The dendritic knobs of receptor cells were positive for anti-G protein alpha-i2 subunit (G ) but negative for anti-G protein alpha-o subunit indicating preferential use of the V1R-G pathway in the vomeronasal system of bears, as in other carnivores. The VNO of the bear possessed three types of secretory cells (secretory cells of the vomeronasal gland, multicellular intraepithelial gland cells and goblet cells), and the present findings showed that the secretory granules in these cells also had various properties. The vomeronasal lumen at the middle region of the VNO invaginated toward the ventral region, and this invagination contained tightly packed multicellular intraepithelial gland cells. To our knowledge, this invagination and intraepithelial gland masses in the VNO are unique features of brown bears. The VNO in the brown bear, especially the secretory system, is morphologically well-developed, suggesting that this organ is significant for information transmission in this species.

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“…The sheep VNO consists of a pair of tubes lined by epithelium placed on both sides of the nasal septum. The VNO and its associated connective tissue, the lamina propria, are surrounded by an incomplete ring of hyaline vomeronasal cartilage [Kratzing, 1971;Ibrahim et al, 2013].…”

Section: Doi: 101159/000487758mentioning

confidence: 99%

Immunolocalization of Receptor and Chemoreceptor Modules in the Sheep Vomeronasal Organ

Ibrahim¹

2018

Cells Tissues Organs

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The vomeronasal organ (VNO) is the peripheral receptor organ of the accessory olfactory system, which is responsible for both sexual and innate behaviors. The degree of neuronal differentiation and maturation of the vomeronasal receptor cells together with the verification of the presence of the solitary chemoreceptor cells (SCCs) in the VNO of Corriedale sheep were assessed using immunofluorescence. A protein gene product 9.5 (PGP 9.5), which is a neuronal marker recognized to be expressed in most neurons of vertebrate species, an olfactory marker protein (OMP) that is precise for mature olfactory receptor cells, and lastly phospholipase C-β₂ (PLC-β₂), a marker in the signal transduction pathway of SCCs, were all tested. The cell bodies and dendrites of almost all receptor cells in the sensory epithelium were strongly positive for PGP 9.5 and to a lesser extent for OMP. In the nonsensory wall, all cells were negative for both PGP 9.5 and OMP; however, some positive PGP 9.5 immunoreactive fibers were identified. For PLC-β₂, only 1 basally situated SCC could be identified in the sensory epithelium. A higher number was demonstrated in the nonsensory wall. Corriedale sheep possess matured, fully differentiated vomeronasal receptor cells in their sensory wall, suggesting an appropriate pheromone perception. Additionally, the VNO in sheep may participate in the usual transduction mechanisms, though it is seemingly not a chemoreceptor organ.

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Lectin Histochemical Studies on the Vomeronasal Organ of the Sheep

Cited by 18 publications

References 25 publications

Histological features of the vomeronasal organ in the giraffe, Giraffa camelopardalis

Histological features of the vomeronasal organ in the giraffe, Giraffa camelopardalis

Morphological and histological features of the vomeronasal organ in the brown bear

Immunolocalization of Receptor and Chemoreceptor Modules in the Sheep Vomeronasal Organ

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