Lectin Histochemistry for Glycoconjugates in the Small Intestines of Piglets Naturally Infected with Isospora suis.

Choi, Bo-Young; Sohn, Yong-Sung; Choi, Changsun; Chae, Chanhee

doi:10.1292/jvms.65.389

Cited by 14 publications

(8 citation statements)

References 12 publications

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“…The inner wall of the esophagus in the present animals show non keratinized stratified squamous epithelium as the same observed of the African Giant Rat [8] (Cricetomys gambianus-Waterhouse, 1840) [10,15].…”

Section: Discussionsupporting

confidence: 73%

Comparative Histological Studies of the Esophagus Wall of Oryctolagus cuniculus Rabbit Adult, Young and Lactating Using Light Microscope

Selim¹,

Hazaa²,

Goda³

2017

J Cytol Histol

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Background: As the different parts of the alimentary canal, the esophagus is an important part of it, because it facilitate the passage of food material to the stomach. The purpose of this study was to compare the structure of the esophageal wall of rabbit before weaning (lactating) and after weaning (young and adult) to determine the structural changes that accompany the changes of the feeding pattern from lactating to adult.

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Section: Discussionsupporting

confidence: 73%

Comparative Histological Studies of the Esophagus Wall of Oryctolagus cuniculus Rabbit Adult, Young and Lactating Using Light Microscope

Selim¹,

Hazaa²,

Goda³

2017

J Cytol Histol

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“…This approach also requires cumbersome isolation and digestion procedures. Therefore, a semiquantitative histochemical lectin binding approach which had been successfully used by others (Chae and Lee, 1995;Chae, 1997;Choi et al, 2003;Freitas et al, 2005) in assessing Table 5. Lectin binding on the dome and corona regions of the ileum in control, antibiotic-fed, and gnotobiotic pigs 1 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 − 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 − VVA 0.17 ± 0.17 0.00 ± 0.00 0.33 ± 0.21 − 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 − Group 2 GSL-II 0.00 ± 0.00 0.00 ± 0.00 0.17 ± 0.17 − 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 − DSL 2.00 ± 0.26 1.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, there is a possibility that some lectins present in the feed may mask the carbohydrate moieties present on the intestinal surfaces and may interfere in their in vitro detection because we did not analyze the presence of specific lectins in the feed in this study. We processed the tissue sections for lectin staining using the procedures that efficiently strip the endogenous ligands from epithelial glycoconjugates and have been successfully used by others (Chae and Lee, 1995;Chae, 1997;Choi et al, 2003;Freitas et al, 2005). All 23 lectins efficiently bound (binding intensities of 1 to 3) to the intestinal epithelium of control pigs, confirming that glycoconjugates were freely available on epithelial surfaces for lectin binding.…”

mentioning

confidence: 91%

“…Pathogens like Campylobacter, Escherichia coli, Vibrio, and commensals like Lactobacillus display surface lectins on their pili/fimbriae that help in their adherence to the intestinal tract (Freter, 1981;Farthing, 1985;Sharon, 1987;Mouricout, 1997). Enteric viruses like rotavirus and protozoans like Cryptosporidia, Entamoeba histolytica, and Isospora suis frequently attach and invade the intestinal epithelial cells by recognizing carbohydrate moieties on the cell surface (Kelly et al, 2000;Sohn and Chae, 2000;Sousa et al, 2001;Stanley and Reed, 2001;Dormitzer et al, 2002;Choi et al, 2003;Tavares et al, 2005). The lower binding intensities of LCA and MAL in antibioticfed animals compared with control animals suggest the lower expression of glucose/mannose and sialic acid moieties in the gut of 3 animals.…”

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confidence: 99%

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Lectin binding profile of the small intestine of five-week-old pigs in response to the use of chlortetracycline as a growth promotant and under gnotobiotic conditions1,2

George

Lindblom

et al. 2007

Journal of Animal Science

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Antibiotics have traditionally been used for growth promotion in the pork industry; however, their use in animal feed has recently been limited because of human health concerns. The intestinal microbiota plays an important role in mediating many physiological functions such as digestion and animal growth. It was hypothesized that use of antibiotics as growth promotants and subsequent variations in intestinal microbiota induce significant changes in the intestinal glycoconjugate composition, which ultimately affects animal growth and disease susceptibility. The aim of this study was to characterize the lectin binding profiles of the ileum of weanling pigs in response to the absence of intestinal microbiota and to the use of the antibiotic chlortetracycline as growth promotant. Eighteen half-sib piglets obtained by cesarean section were divided into 3 treatment groups (n = 6) and maintained as control, antibiotic-fed, and gnotobiotic piglets until 5 wk of age. The glycoconjugate composition of the ileal tissues was examined by lectin histochemistry. Lycopersicon esculentum lectin, Jacalin, Pisum sativum agglutinin, Lens culinaris agglutinin (LCA), and Sambucus nigra lectin showed significant differences (P < 0.05) in binding intensities on the dome and villous epithelium between the treatment groups. Griffonia simplicifolia lectin I, Glycine maxi agglutinin, and Arachis hypogea agglutinin exhibited differences (P < 0.05) between treatment groups in lectin binding on goblet cells. Triticum vulgaris agglutinin, Pisum sativum agglutinin, and LCA showed significant differences (P < 0.05) in binding intensities on dome, corona, and follicular regions of the ileum among treatment groups of animals. Overall, ileal tissues from gnotobiotic piglets expressed significantly weaker (P < 0.05) lectin binding for many lectins compared with control and antibiotic groups. This suggests that the intestinal microbiota plays an important role in the expression of sugar moieties in the intestine. Lectins LCA, Phaseolus vulgaris Leucoagglutinin, and Maackia amurensis lectin II showed significant differences (P < 0.05) in lectin bindings between control and antibiotic-fed piglets. This indicates that chlortetracycline as a growth promotant induces biologically relevant changes in the lectin binding profile of the ileum. These findings will help in further understanding the role of the gut microbiota and the mechanisms of action of antibiotics as growth promotants in pigs.

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“…In suckling piglets, I. suis occurred with lower percentage (8.9 %) than previously reported (44.7 %) in the province of Havana by Koudela et al (1989); the housing improvement carried out in Cuban piggeries during last years could contribute to this difference. The 66.6 % of I. suis positive pigs was also infected with ETEC, Choi et al (2003) stated that isosporosis could promote intestinal colonization by ETEC due to an increase of glycoconjugates on the jejunal enterocytes.…”

Section: Discussionmentioning

confidence: 99%

Several enteropathogens are circulating in suckling and newly weaned piglets suffering from diarrhea in the province of Villa Clara, Cuba

Rodríguez

Martín

Muñoz

et al. 2012

Trop Anim Health Prod

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Intestinal contents of suckling (n = 45) and newly weaned (n = 45) piglets, suffering from diarrhea in the province of Villa Clara in Cuba, were tested for viral, bacterial, and parasitic enteropathogens from May to June 2008. At least one enteropathogen was identified in 53.3 % of piglets and enterotoxigenic Escherichia coli (ETEC; 25.6 %) was the major pathogen; mostly STa(+)/STb(+) or F4(+)/STa(+)/STb(+) ETEC were isolated. The overall occurrence of the rest of pathogens was 10 % for transmissible gastroenteritis virus (TGEV) and Cryptosporidium parvum, 6.7 % for rotavirus A and Isospora suis, 5.6 % for α-toxigenic Clostridium perfringens, 3.3 % for verotoxigenic E. coli (VTEC), and 2.2 % for Salmonella enterica subspecies enterica serovar Newport. TGEV and α-toxigenic C. perfringens were only identified in suckling piglets, while Salmonella Newport and VTEC were only detected in weaned pigs. Porcine epidemic diarrhea virus (PEDV), β-toxigenic C. perfringens, Eimeria spp., and helminths were not identified. Eight kinds of mixed infections were detected in 25 % of enteropathogen positive piglets. ETEC was present in 10 of 12 mixed infections, and TGEV infections were never combined. This survey demonstrates that several enteropathogens are circulating in piggeries located in the province of Villa Clara in Cuba, and that is necessary to improve surveillance, prevention, and control of enteric infections in order to increase production efficiency.

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Lectin Histochemistry for Glycoconjugates in the Small Intestines of Piglets Naturally Infected with Isospora suis.

Cited by 14 publications

References 12 publications

Comparative Histological Studies of the Esophagus Wall of Oryctolagus cuniculus Rabbit Adult, Young and Lactating Using Light Microscope

Comparative Histological Studies of the Esophagus Wall of Oryctolagus cuniculus Rabbit Adult, Young and Lactating Using Light Microscope

Lectin binding profile of the small intestine of five-week-old pigs in response to the use of chlortetracycline as a growth promotant and under gnotobiotic conditions1,2

Several enteropathogens are circulating in suckling and newly weaned piglets suffering from diarrhea in the province of Villa Clara, Cuba

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