Leishmania (Viannia) braziliensis genotypes identified in lesions of patients with atypical or typical manifestations of tegumentary leishmaniasis: Evaluation by two molecular markers

Baptista, Cibele; Schubach, Armando; Madeira, M.F.; Leal, Cristianni Antunes; Pires, Marize Quinhones; Oliveira, Fernanda Santos de; Conceição-Silva, Fátima; Rosalino, Cláudia Maria Valete; Salgueiro, Mariza M; Pacheco, Raquel S.

doi:10.1016/j.exppara.2008.12.006

Cited by 32 publications

(34 citation statements)

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“…It is caused by Leishmania species of the subgenera Leishmania ( Viannia ) and ( Leishmania ) and is distributed from the south of the United States to the north of Argentina [2, 7]. In Brazil, ATL is detected in all states and has shown a high incidence over the last 20 years.…”

Section: Introductionmentioning

confidence: 99%

“…There is wide genetic diversity among the Leishmania parasites; at least seven Leishmania species have been described as the etiological agent of human cutaneous disease, with most cases being caused by Leishmania ( Viannia ) braziliensis . However, L. amazonensis is also important as a causative agent of ATL, having been reported in the northeast, southeast, and west-central parts of Brazil [7–9]. …”

Section: Introductionmentioning

confidence: 99%

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Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

Queiroz

Dias

Matta

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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This study investigates the leishmanicidal activity of five species of plants used in folk medicine in endemic areas of the state of Alagoas, Brazil. Data were collected in the cities of Colonia Leopoldina, Novo Lino, and União dos Palmares, Alagoas state, from patients with cutaneous leishmaniasis (Leishmania amazonensis) who use medicinal plants to treat this disease. Plants extracts were tested at a concentration of 1–100 μg/mL in all experiments, except in an assay to evaluate activity against amastigotes, when 10 μg/mL was used. All plants extracts did not show deleterious activity to the host cell evidenced by LDH assay at 100, 10, and 1 μg/mL after 48 h of incubation. The plants extracts Hyptis pectinata (L.) Poit, Aloe vera L., Ruta graveolens L., Pfaffia glomerata (Spreng.) Pedersen, and Chenopodium ambrosioides L. exhibited direct activity against extracellular forms at 100 μg/mL; these extracts inhibited growth by 81.9%, 82.9%, 74.4%, 88.7%, and 87.4%, respectively, when compared with promastigotes. The plants extracts H. pectinata, A. vera, and R. graveolens also significantly diminished the number of amastigotes at 10 μg/mL, inhibiting growth by 85.0%, 40.4%, 94.2%, and 97.4%, respectively, when compared with control. Based on these data, we conclude that the five plants exhibited considerable leishmanicidal activity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

Queiroz

Dias

Matta

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…( V .) braziliensis in ATL patients [20,21]. The purpose of the present study is to further investigate parasite subpopulations, with a particular focus on the genetic variability of L .…”

Section: Introductionmentioning

confidence: 99%

American tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis: assessment of parasite genetic variability at intra- and inter-patient levels

et al. 2013

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BackgroundThe genetic variability of Leishmania (Viannia) braziliensis was assessed at intra and interpatient levels of individuals with different clinical manifestations of American tegumentary leishmaniasis (ATL).MethodsFifty-two samples, of which 13 originated from cutaneous lesions and 39 from mucosal lesions, provided by 35 patients, were examined by low-stringency single-specific-primer PCR (LSSP-PCR) and phenetic analysis. Genetic variability of L. (V.) braziliensis, in kinetoplast DNA (kDNA) signatures, was compared both from different patients and from different lesions of the same patient. Phenetic analysis was performed to evaluate the degree of heterogeneity of the kDNA minicircles. In order to evaluate inter and intrapatient L. (V.) braziliensis genetic variability, the percentage of shared bands and analysis of the coefficients of similarity were analyzed.ResultsDifferent genetic profiles, representing kDNA signatures of the parasite, were obtained by LSSP-PCR analysis of each sample. Phenetic analysis grouped genetic profiles of different levels of differentiation from more similar to most divergent. The percentage of shared bands at the inter and intrapatient levels was 77% and 89%, respectively. Comparison of the average inter and intrapatient coefficients of similarity and their standard deviations were statistically significant (p < 0.001).ConclusionGenetic variability at the intrapatient level was less pronounced than that between different patients. A conceptual model was proposed to better understand the complexity at both levels.

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“…One of the disadvantages of this technique is the need for previous isolation of the parasite to subsequently obtain a large culture volume, a fact that has contributed to the use of PCR-based methods in many studies 26,27 . However, the variability of parasites of the genus Leishmania has been widely studied by MLEE 11,19,28 .…”

Section: Ethical Considerationsmentioning

confidence: 99%

Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis

Coutinho

Santos

Baptista

et al. 2011

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Domestic dogs are the most important reservoir in the peridomestic transmission cycle of Leishmania (Leishmania) chagasi. The genetic variability of subpopulations of this parasite circulating in dogs has not been thoroughly analyzed in Brazil, even though this knowledge has important implications in the clinical-epidemiological context. METHODS: The objective of this study was to evaluate and compare the phenotypic variability of 153 L. chagasi strains isolated from dogs originating from the municipalities of Rio de Janeiro (n = 57) and Belo Horizonte (n = 96), where the disease is endemic. Strains isolated only from intact skin were selected and analyzed by multilocus enzyme electrophoresis using nine enzyme systems (6PG, GPI, NH1 and NH2, G6P, PGM, MDH, ME, and IDHNADP). RESULTS: The electrophoretic profile was identical for all isolates analyzed and was the same as that of the L. chagasi reference strain (MHOM/BR/74/PP75). Phenetic analysis showed a similarity index of one for all strains, with the isolates sharing 100% of the characteristics analyzed. CONCLUSIONS: The results demonstrate that the L. chagasi populations circulating in dogs from Rio de Janeiro and Belo Horizonte belong to a single zymodeme.

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Leishmania (Viannia) braziliensis genotypes identified in lesions of patients with atypical or typical manifestations of tegumentary leishmaniasis: Evaluation by two molecular markers

Cited by 32 publications

References 35 publications

Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

American tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis: assessment of parasite genetic variability at intra- and inter-patient levels

Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis

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