Length Scale Dependence of the Dynamic Properties of Hyaluronic Acid Solutions in the Presence of Salt

Horkay, Ferenc; Falus, Péter; Hecht, Anne‐Marie; Geissler, Erik

doi:10.1021/jp106578f

Cited by 11 publications

(21 citation statements)

References 16 publications

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“…In the presence of BC‐dots, the positively charged CS@AuNPs agglomerated, and the fluorescence intensity of BC‐dots decreased ( I ). HA inhibited the agglomeration of CS@AuNPs induced by C‐dots, due to its semirigid coil conformation and high affinity for CS@AuNPs. Consequently, C‐dot fluorescence was further quenched by the monodispersed HA‐CS@AuNPs ( II ).…”

Section: Resultssupporting

confidence: 92%

A dual‐readout nanosensor based on biomass‐based C‐dots and chitosan@AuNPs with hyaluronic acid for determination of hyaluronidase

et al. 2019

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A dual-signal strategy is proposed based on fluorescent biomass-based carbon dots (BC-dots) and chitosan stabilized AuNPs (CS@AuNPs) to determine hyaluronidase (HAase). BC-dots can induce aggregation of CS@AuNPs nanoparticles with a colour change from red to blue. Positively charged CS@AuNPs interacted with the negatively charged hyaluronic acid (HA) through electrostatic adsorption, and CS@AuNPs maintained stability due to the semirigid coil conformation of HA. However, in the presence of HAase, due to enzymatic hydrolysis of HA by HAase, the CS@AuNPs agglomerated. Based on the change of fluorescence and colour, quantitative analysis of HAase was achieved. Linear ranges for the fluorometric and colorimetric determinations were 2.0-70 U mL −1 and 8-60 U mL −1 , respectively, with a detection limit of 0.27 U mL −1 . This dual-signal sensing system possesses high potential for determination of HAase in biological matrices.

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Section: Resultssupporting

confidence: 92%

A dual‐readout nanosensor based on biomass‐based C‐dots and chitosan@AuNPs with hyaluronic acid for determination of hyaluronidase

et al. 2019

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“…53 Finally, hydrated polymers and polymer solutions-in particular, microgels-have also recently been investigated by QENS. [54][55][56][57][58][59][60][61][62][63] Studies on concentrated aqueous solutions show that confinement effects also arise -in this case for the minority water component-when the polymer freezes approaching its glass-transition. The component dynamics in these solutions can be selectively monitored by QENS, a task that is not easily achievable by other usually applied experimental approaches like, for example, dielectric spectroscopy.…”

Section: Recent Progress On Polymer Dynamics By Neutron Scatteringmentioning

confidence: 99%

“…Considering now polyelectrolyte solutions, they also remain incompletely understood due to several reasons: their multicomponent nature, their structural complexity-that can be even increased with the polymer architecture-, and the presence of short-and long-range interactions. The investigation carried out by Horkay et al 57 was conducted on semidiluted solutions of a semirigid biological polyelectrolyte: the sodium salt of hyaluronic acid, one of the most abundant biopolymers in the human body. In this polymer, the local configuration is simpler than that of more flexible polyelectrolytes.…”

Section: Figure 12mentioning

confidence: 99%

Recent progress on polymer dynamics by neutron scattering: From simple polymers to complex materials

Colmenero

Arbe

2012

J Polym Sci B Polym Phys

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ABSTRACT:The recent (from 2010 onward) contributions of quasielastic neutron scattering techniques (time of flight, backscattering, and neutron spin echo) to the characterization and understanding of dynamical processes in soft materials based on polymers are analyzed. The selectivity provided by the combination of neutron scattering and isotopic-in particular, proton/deuterium-labeling allows the isolated study of chosen molecular groups and/or components in a system. This opportunity, together with the capability of neutrons to provide space/time resolution at the relevant length scales in soft matter, allows unraveling the nature of the large variety of molecular motions taking place in materials of increasing complexity. As a result, recent relevant works can be found dealing with dynamical process which associated characteristic lengths and nature are as diverse as, for example, phenyl motions in a glassy linear homopolymer like polystyrene and the chain dynamics of a polymer adsorbed on dispersed clay platelets. © 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 51: 2013 KEYWORDS: molecular dynamics; neutron scattering; relaxation POLYMER DYNAMICS AT DIFFERENT LENGTH AND TIME SCALES: FROM SIMPLE POLYMERS TO COMPLEX MATERIALSPolymers are composed of macromolecules which are built up by a large number N of monomer units linked together by covalent bonds. Because of this macromolecular nature, the structural and dynamic properties of polymeric systems strongly depend on a hierarchy of length and time scales. From a structural point of view, the random coil shape of linear polymer chains in the melt and glassy state-proposed by Flory in the 50s 1 -is nowadays well established by small angle neutron diffraction (SANS). At smaller length scales than those characteristic for the chain dimensions, the local arrangements of the atoms give rise to broad maxima in the static structure factor S(Q) also accessible by neutron diffraction. Usually, a first main peak centered at Q-values ≈ 1 . . . 1.5 Å −1 is present. The T-dependence of this maximum, following the expansion coefficient, indicates an interchain origin, that is, it mainly relates to correlations between atoms belonging to different chains (or to the same chain but well separated segments).2 A second peak located at ∼3 Å −1can also be usually found in S(Q). The small value of the associated characteristic length and its weak T-dependence indicate the intrachain nature of the correlations giving rise to this maximum. We also note that the broadening of the Bragg peaks found in polymers reveals a clear amorphous character. In fact, the features shown in S(Q) in polymeric systems are universal for amorphous materials, not only for polymers, and in particular for glass forming systems. Thus, a universal behavior common for all glass forming materials can also be expected in the dynamics, including the observation of the glass transition phenomenon. 3As anticipated, the complexity of amorphous polymers shows up not only in the structural propertie...

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“…The slower relaxation mode in the bimodal distribution is nondiffusive (the relaxation rate, Γ, follows a power law of ∼ q 3 ; see Figure S7) and also appears in samples of pure NaHA. It can be attributed to the generally observed “slow mode” of polyelectrolytes, seen in particular at a low ionic strength, which has been attributed to the presence of supramolecular structures or hindered motions of interacting chains. , Such a slow mode with q 3 scaling has been reported also for hyaluronate before . Because of the lower charge density, NaHA has to be employed in higher weight fractions than NaPA to achieve the same mixing ratios.…”

Section: Resultsmentioning

confidence: 78%

Effect of Polymer Architecture on the Phase Behavior and Structure of Polyelectrolyte/Microemulsion Complexes (PEMECs)

et al. 2020

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We studied polyelectrolyte/microemulsion complexes (PEMECs) formed by the biopolymer sodium hyaluronate (NaHA) and cationic oil-in-water (O/W) microemulsion droplets. Around equimolar charge conditions, a two-phase region with a liquid−liquid phase separation (coacervate formation) is observed, while mixed complexes are formed at polyelectrolyte excess. The largest complexes are found close to the phase boundary. The detailed structure of these complexes was determined by a combination of static and dynamic light scattering (SLS and DLS), small-angle neutron scattering (SANS), and cryo-transmission electron cryomicroscopy (cryo-TEM). Interestingly, these complexes formed with NaHA are much more elongated compared to previously studied complexes formed with sodium polyacrylate (NaPA). Similar observations were made for another polysaccharide, sodium carboxymethyl cellulose (NaCMC). Apparently, the persistence length of the complexes is largely proportional to the persistence length of the polyelectrolyte, which was determined by analyzing the SANS data with a specifically developed model. Accordingly, the size of the complexes formed with stiffer biopolyelectrolytes becomes much larger, increasing with the molecular weight (M w ) of the polyelectrolyte. In summary, we conclude that the size and structure of the PEMECs can be controlled by the type of polyelectrolyte, mixing ratio, size of the microemulsion droplets, and M w of the polyelectrolyte. This means that the polyelectrolyte addition can be used as a key structuring element for the size and shape of such complexes.

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Length Scale Dependence of the Dynamic Properties of Hyaluronic Acid Solutions in the Presence of Salt

Cited by 11 publications

References 16 publications

A dual‐readout nanosensor based on biomass‐based C‐dots and chitosan@AuNPs with hyaluronic acid for determination of hyaluronidase

A dual‐readout nanosensor based on biomass‐based C‐dots and chitosan@AuNPs with hyaluronic acid for determination of hyaluronidase

Recent progress on polymer dynamics by neutron scattering: From simple polymers to complex materials

Effect of Polymer Architecture on the Phase Behavior and Structure of Polyelectrolyte/Microemulsion Complexes (PEMECs)

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