Lentiviral transgenesis of the leopard gecko, Eublepharis macularius

Hull, Kaitlyn; Hodgson, Dee; Clark, Bob; Hickok, Timothy W.; Petitte, James N.; Mozdziak, Paul E.

doi:10.5897/ajmr2013.6532

Cited by 3 publications

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Injected or manipulated chicken embryos have been cultured until hatch using surrogate egg-shell technique which has been efficient enough to keep manipulated embryos survived until hatch. Recently there has been some ideas about production of transgenic reptiles such as snakes (Mozdziak & Petitte, 2010) and gecko (Kaitlyn et al, 2014) to produce transgenic reptiles to be used as a model to test oncogenic and teratogenic agents (Patent No. US 8,134,044 B2, 2012).…”

Section: Characterization Of Pgcsmentioning

confidence: 99%

“…Another study on leopard gecko, E. macularius using lentiviral vector injection showed a successful production of transgenic reptiles. Leopard gecko as a captivated reptile is claimed to be a good model for transgenesis as they produce large number of embryos and Lentiviral vector injection is easier through their soft eggshell (Kaitlyn et al, 2014). Lentiviral or retroviral vectors also were injected to the snake eggs and successfully produced transgenic snakes (Mozdziak & Petitte, 2010).…”

Section: Characterization Of Pgcsmentioning

confidence: 99%

See 1 more Smart Citation

In vitro culture of reptile PGCS to preserve endangered species

Golkar‐Narenji

Dzięgiel

Kempisty

et al. 2023

Cell Biology International

View full text Add to dashboard Cite

Primordial germ cells (PGCs), are the source of gametes in vertebrates. There are similarities in the development of PGCs of reptiles with avian and mammalian species PGCs development. PGCs culture has been performed for avian and mammalian species but there is no report for reptilian PGCs culture. In vitro culture of PGCs is needed to produce transgenic animals, preservation of endangered animals and for studies on cell behaviour and research on fertility. Reptiles are traded as exotic pets and a source of food and they are valuable for their skin and they are useful as model for medical research. Transgenic reptile has been suggested to be useful for pet industry and medical research. In this research different aspects of PGCs development was compared in three main classes of vertebrates including mammalian, avian and reptilian species. It is proposed that a discussion on similarities between reptilian PGCs development with avian and mammalian species helps to find clues for studies of reptilian PGCs development details and finding an efficient protocol for in vitro culture of reptilian PG.

show abstract

Section: Characterization Of Pgcsmentioning

confidence: 99%

Section: Characterization Of Pgcsmentioning

confidence: 99%

In vitro culture of reptile PGCS to preserve endangered species

Golkar‐Narenji

Dzięgiel

Kempisty

et al. 2023

Cell Biology International

View full text Add to dashboard Cite

show abstract

Reptile assisted reproductive technologies: can ART help conserve 300 million years of evolution by preserving extant reptile biodiversity?

Perry

Mitchell

2021

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Biodiversity loss is the greatest environmental problem threatening ecosystem, animal, and human health. Anthropogenic induced changes to climate, habitat, disease, species distributions, poaching, and unsustainable trade have accelerated extinction rates in all vertebrates, including reptiles. Preventing reptile extinctions will require humans to acknowledge these losses and develop ex situ and in situ plans to preserve them. Assisted reproductive technologies (ART) are management tools used to protect numerous vertebrate taxa; however, progress in developing ART for reptiles has lagged. Creating functional and sustainable reptile ART will strengthen our conservation capacity by capturing genetic material from select individuals to overcome natural or manmade boundaries. Utilising short-term gamete storage and genome resource banking, in conjunction with timed artificial insemination (AI) or ex ovo incubation, could lead to profound advances in reptile conservation, mitigating the loss of reptile biodiversity. In this article, we review ART reptile research completed since the 1970s. Topics include AI, hormonal control of reproduction, gamete collection, gamete storage, and genome resource banking. Additionally, we review the potential application of advanced reproductive methodologies, including in vitro/ex ovo fertilisation, intracytoplasmic sperm injection, cloning (somatic cell nuclear transfer), and genetic editing.

show abstract

Skull Development, Ossification Pattern, and Adult Shape in the Emerging Lizard Model Organism Pogona vitticeps: A Comparative Analysis With Other Squamates

et al. 2018

View full text Add to dashboard Cite

The rise of the Evo-Devo field and the development of multidisciplinary research tools at various levels of biological organization have led to a growing interest in researching for new non-model organisms. Squamates (lizards and snakes) are particularly important for understanding fundamental questions about the evolution of vertebrates because of their high diversity and evolutionary innovations and adaptations that portrait a striking body plan change that reached its extreme in snakes. Yet, little is known about the intricate connection between phenotype and genotype in squamates, partly due to limited developmental knowledge and incomplete characterization of embryonic development. Surprisingly, squamate models have received limited attention in comparative developmental studies, and only a few species examined so far can be considered as representative and appropriate model organism for mechanistic Evo-Devo studies. Fortunately, the agamid lizard Pogona vitticeps (central bearded dragon) is one of the most popular, domesticated reptile species with both a well-established history in captivity and key advantages for research, thus forming an ideal laboratory model system and justifying his recent use in reptile biology research. We first report here the complete post-oviposition embryonic development for P. vitticeps based on standardized staging systems and external morphological characters previously defined for squamates. Whereas the overall morphological development follows the general trends observed in other squamates, our comparisons indicate major differences in the developmental sequence of several tissues, including early craniofacial characters. Detailed analysis of both embryonic skull development and adult skull shape, using a comparative approach integrating CT-scans and gene expression studies in P. vitticeps as well as comparative embryology and 3D geometric morphometrics in a large dataset of lizards and snakes, highlights the extreme adult skull shape of P. vitticeps and further indicates that heterochrony has played a key role in the early development and ossification of squamate skull bones. Such detailed studies of embryonic character development, craniofacial patterning, and bone formation are essential for the establishment of well-selected squamate species as Evo-Devo model organisms. We expect that P. vitticeps will continue to emerge as a new attractive model organism for understanding developmental and molecular processes underlying tissue formation, morphology, and evolution.

show abstract

Lentiviral transgenesis of the leopard gecko, Eublepharis macularius

Cited by 3 publications

References 43 publications

In vitro culture of reptile PGCS to preserve endangered species

In vitro culture of reptile PGCS to preserve endangered species

Reptile assisted reproductive technologies: can ART help conserve 300 million years of evolution by preserving extant reptile biodiversity?

Skull Development, Ossification Pattern, and Adult Shape in the Emerging Lizard Model Organism Pogona vitticeps: A Comparative Analysis With Other Squamates

Contact Info

Product

Resources

About