Lethal and mutagenic interactions between γ-rays, cisplatin and etoposide at the cellular and molecular levels

Lillo, Olga; Bracesco, Nélson; Nunes, Elia

doi:10.3109/09553002.2010.518207

Cited by 5 publications

(3 citation statements)

References 35 publications

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“…etoposide, pactitaxel, and Vinca alkaloids, which have not only been recognized as potential radiosensitizers, but already under clinical application in association with cancer radiotherapy (Burris and Hurtig, 2010). Nevertheless, these drugs and their analogs are also under continuous appraisal for further development in this regard (Hiro et al, 2010; Orditura et al, 2010; Lillo et al, 2011; Schwarzenberger et al, 2011). …”

Section: Potential Plant Products For Application In Cancer Radiotherapymentioning

confidence: 99%

The Prospective Role of Plant Products in Radiotherapy of Cancer: A Current Overview

Hazra¹,

Ghosh²,

Kumar³

et al. 2012

Front. Pharmacol.

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Treatment of cancer often requires exposure to radiation, which has several limitations involving non-specific toxicity toward normal cells, reducing the efficacy of treatment. Efforts are going on to find chemical compounds which would effectively offer protection to the normal tissues after radiation exposure during radiotherapy of cancer. In this regard, plant-derived compounds might serve as “leads” to design ideal radioprotectors/radiosensitizers. This article reviews some of the recent findings on prospective medicinal plants, phytochemicals, and their analogs, based on both in vitro and in vivo tumor models especially focused with relevance to cancer radiotherapy. Also, pertinent discussion has been presented on the molecular mechanism of apoptotic death in relation to the oxidative stress in cancer cells induced by some of these plant samples and their active constituents.

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Section: Potential Plant Products For Application In Cancer Radiotherapymentioning

confidence: 99%

The Prospective Role of Plant Products in Radiotherapy of Cancer: A Current Overview

Hazra¹,

Ghosh²,

Kumar³

et al. 2012

Front. Pharmacol.

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“…Based on survival curves ( Figure 1 ) an absorbed dose of 200 Gy was selected for combination treatments. Aliquots of cells were plated in solid nutrient medium YPDA: YPD + 2% agar (DIFCO Laboratories, USA) and incubated at 30°C for 72 h. Survival was calculated as surviving fraction: S(x,y) = Ns/No, where Ns is the number of surviving cells capable of generating visible clones/mL; No is the total number of treated cells/mL; x the absorbed dose of radiation; and y the doses of the putative protectors ( 16 , 17 ).…”

Section: Methodsmentioning

confidence: 99%

“…To determine mutation frequency, cell samples of SC7K lys2-3 were plated after treatment on omission media (OM: 2% dextrose, 0.67% nitrogenous base yeast (Sigma-Aldrich, USA), 2% agar) ( 17 , 18 ) and incubated at 30°C for 21 days. Thereafter, the number of revertants lys → LYS were scored ( 12 , 13 ).…”

Section: Methodsmentioning

confidence: 99%

Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin

Bracesco

Sosa

Blanc

et al. 2018

Braz J Med Biol Res

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DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were determined after treatments. A significant increase in surviving fractions after gamma irradiation was observed following combined exposure to γ+R, or γ+Ip. Upon these concomitant treatments, mutation and DSB frequency decreased significantly. In the mutant strain deficient in MEC1, a significant increase in γ sensitivity and a low effect of rutin on γ-induced chromosomal fragmentation was observed. Results were interpreted in the framework of a model of interaction between radiation-induced free radicals, DNA repair pathways, and checkpoint controls, where the DNA damage that induced activation of MEC1 nodal point of the network could be modulated by Ip components including rutin. Furthermore, ionizing radiation-induced redox cascades can be interrupted by rutin potential and other protectors contained in Ip.

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Saccharomyces cerevisiae as a model system to study the response to anticancer agents

Matuo

Sousa

Soares

et al. 2012

Cancer Chemother Pharmacol

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The development of new strategies for cancer therapeutics is indispensable for the improvement of standard protocols and the creation of other possibilities in cancer treatment. Yeast models have been employed to study numerous molecular aspects directly related to cancer development, as well as to determine the genetic contexts associated with anticancer drug sensitivity or resistance. The budding yeast Saccharomyces cerevisiae presents conserved cellular processes with high homology to humans, and it is a rapid, inexpensive and efficient compound screening tool. However, yeast models are still underused in cancer research and for screening of antineoplastic agents. Here, the employment of S. cerevisiae as a model system to anticancer research is discussed and exemplified. Focusing on the important determinants in genomic maintenance and cancer development, including DNA repair, cell cycle control and epigenetics, this review proposes the use of mutant yeast panels to mimic cancer phenotypes, screen and study tumor features and synthetic lethal interactions. Finally, the benefits and limitations of the yeast model are highlighted, as well as the strategies to overcome S. cerevisiae model limitations.

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Lethal and mutagenic interactions between γ-rays, cisplatin and etoposide at the cellular and molecular levels

Cited by 5 publications

References 35 publications

The Prospective Role of Plant Products in Radiotherapy of Cancer: A Current Overview

The Prospective Role of Plant Products in Radiotherapy of Cancer: A Current Overview

Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin

Saccharomyces cerevisiae as a model system to study the response to anticancer agents

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