Lethal photosensitization of periodontal pathogens by a red‐filtered Xenon lamp <i>in vitro</i>

Matevski, Donco; Weersink, Robert A.; Tenenbaum, Howard C.; Wilson, Brian C.; Ellen, Richard P.; Lépine, Guylaine

doi:10.1034/j.1600-0765.2003.00673.x

Cited by 76 publications

(54 citation statements)

References 43 publications

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“…Matevski et al (28) found that a red-filtered xenon lamp was at least as effective as a laser for the photodynamic killing of P. gingivalis in vitro. Nevertheless, it is not known whether a single-wavelength laser or a broadspectrum light source would have provided better PDT effects in our study because multiple factors, such as O 2 saturation and serum in tissue, might affect the outcome of the therapy.…”

Section: Vol 49 2005 Pdt For Vibrio Vulnificus Infection 899mentioning

confidence: 99%

“…However, considerable heat will be generated at a low fluence rate with a broad-spectrum light source, such as the PDT-1200 lamp. Matevski et al (28) found that the heat generated with a red-filtered xenon lamp might compromise future clinical applications for the treatment of periodontal infections. Continuous cooling of the irradiated surface is thus necessary to lessen the hyperthermic effects and discomfort generated during irradiation with a fluence rate greater than 40 mW/cm 2 with the PDT-1200 lamp (T. W. Wong, unpublished data).…”

Section: Vol 49 2005 Pdt For Vibrio Vulnificus Infection 899mentioning

confidence: 99%

See 1 more Smart Citation

Bactericidal Effects of Toluidine Blue-Mediated Photodynamic Action on Vibrio vulnificus

Wong

Wang

Sheu³

et al. 2005

Antimicrob Agents Chemother

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Vibrio vulnificus is a gram-negative, highly invasive bacterium responsible for human opportunistic infections. We studied the antibacterial effects of toluidine blue O (TBO)-mediated photodynamic therapy (PDT) for V. vulnificus wound infections in mice. Fifty-three percent (10 of 19) of mice treated with 100 g of TBO per ml and exposed to broad-spectrum red light (150 J/cm 2 at 80 mW/cm 2 ) survived, even though systemic septicemia had been established with a bacterial inoculum 100 times the 50% lethal dose. In vitro, the bacteria were killed after exposure to a lower light dose (100 J/cm 2 at 80 mW/cm 2 ) in the presence of low-dose TBO (0.1 g/ml). PDT severely damaged the cell wall and reduced cell motility and virulence. Cell-killing effects were dependent on the TBO concentration and light doses and were mediated partly through the reactive oxygen species generated during the photodynamic reaction. Our study has demonstrated that PDT can cure mice with otherwise fatal V. vulnificus wound infections. These promising results suggest the potential of this regimen as a possible alternative to antibiotics in future clinical applications.Photodynamic therapy (PDT) is an experimental treatment which shows great potential for the treatment of neoplastic and nonneoplastic diseases (11,12). PDT involves a light-sensitive photosensitizer, light, and molecular oxygen (14). After excitation with visible light, highly cytotoxic singlet oxygen and other reactive oxygen species (ROS) are generated by either energy or electron transfer (14,30,32). The use of photosensitizers for microbial eradication can be traced back to before the age of chemotherapy (16,38). The antimicrobial effects of PDT are increasingly recognized. The technique has been shown to have effects against a range of oral pathogens and also against drug-resistant bacteria. More importantly, there have been reports of the use of PDT to treat infections in selected animal models (13,15,17) and some clinical trials (16), with encouraging results. The activities of the virulence factors of gram-negative bacteria were also reduced with PDT (24). Among the many photosensitizers, toluidine blue O (TBO), a cationic phenothiazinium photosensitizer (16,38), has been shown to be phototoxic to gram-negative bacilli with red-light irradiation (39). However, in vivo antimicrobial studies of TBO-PDT were mainly limited to oral infections (39).Vibrio vulnificus is a gram-negative, motile, curved bacillus of the family Vibrionaceae (22). Most Vibrio species are free-living in marine or brackish water (6). Many cases of V. vulnificus infection have been reported from the coastal areas of the United States (4), Asia (6), and Europe (10). V. vulnificus causes primary sepsis, wound infection, and gastrointestinal illness in humans (6,22). This organism is extremely virulent, and infections with this organism typically occur in patients with underlying liver disease 1 to 2 days after exposure. The mortality rate is up to 55% in septic patients and 25% in those with wound infect...

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Section: Vol 49 2005 Pdt For Vibrio Vulnificus Infection 899mentioning

confidence: 99%

Section: Vol 49 2005 Pdt For Vibrio Vulnificus Infection 899mentioning

confidence: 99%

Bactericidal Effects of Toluidine Blue-Mediated Photodynamic Action on Vibrio vulnificus

Wong

Wang

Sheu³

et al. 2005

Antimicrob Agents Chemother

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“…14 In endogenous PDT, the PDT e®ect depended on the endogenous photosensitizer porphyrin. [15][16][17] Endogenous PDT was safer than the exogenous one, because exogenous photosensitizer might have chemical toxicity and cause more side e®ects. 18,19 Endogenous PDT was much more commonly and e®ectively used in acne therapy.…”

Section: Discussionmentioning

confidence: 99%

Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

Jiang

Fan

et al. 2015

J. Innov. Opt. Health Sci.

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Photodynamic therapy (PDT) has been commonly used in treating many diseases, such as cancer and infectious diseases. We investigated the di®erent e®ects of PDT on three main pathogenic bacteria of periodontitis -Prevotella melaninogenica (P.m.), Porphyromonas gingivalis (P.g.) and Aggregatibacter actinomycetemcomitans (A.a.). The portable red light-emitting diode (LED) phototherapy device was used to assess the exogenous PDT e®ects with di®erent light doses and photosensitizer concentrations (Toluidine blue O, TBO). The portable blue LED phototherapy device was used to assess the endogenous PDT e®ects with the use of endogenous photosensitizers (porphyrin) under di®erent light doses. We found out that both exogenous and endogenous PDT were able to restrict the growth of all the three bacteria signi¯cantly. Moreover, the optimal PDT conditions for these bacteria were obtained through this in vitro screening and could guide the clinical PDT on periodontitis.

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“…The in vitro bactericidal effect of PDT on Gram-negative bacteria was also investigated and it was found to have a high bactericidal effect on Haemophilus parainfluenzae 34 , Prevotella and Porphyromonas species [35][36][37] , Helicobacter mustelae 38 , E. coli B, Acinetobacter baumannii, and P. aeruginosa 39,40 . PDT of Gram-positive and Gram-negative bacteria in animal models hasproven to be as successful as in in vitro studies in inactivating the causative bacteria [41][42][43][44][45][46] .…”

Section: Prophylactic Antimicrobials and Resistancementioning

confidence: 99%

Surgical site infections subjected to photodynamic therapy: a potential application in orthopaedic surgery

Jerjes¹,

Tan²,

Hopper³

et al. 2012

Hard Tissue

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Introduction Photodynamic therapy, a minimally invasive oncological modality, has been in use for over 20 years. However, little clinical data is available on its non-oncological efficacy. Several laboratory-based trials on animals have suggested that this technology may be applicable as an antimicrobial therapy. In orthopaedics, where implants and metal work are placed in deep tissue planes, a potential risk of infection is treated very seriously. Infection not only increases patient morbidity and mortality but also the burden on healthcare system. Proposing a new modality that can instantly tackle this problem. This critical review discusses surgical site infections subjected to photodynamic therapy. Conclusion Surgical care should be state of the art with careful attention to strategies that avoid the development of SSIs. When SSI does occur, superficial and deep wound infections can be treated using PDT by applying topical photosensitiser to the area followed by light illumination.

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Lethal photosensitization of periodontal pathogens by a red‐filtered Xenon lamp in vitro

Cited by 76 publications

References 43 publications

Bactericidal Effects of Toluidine Blue-Mediated Photodynamic Action on Vibrio vulnificus

Bactericidal Effects of Toluidine Blue-Mediated Photodynamic Action on Vibrio vulnificus

Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

Surgical site infections subjected to photodynamic therapy: a potential application in orthopaedic surgery

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