LetR is a TetR family transcription factor from Lysobacter controlling antifungal antibiotic biosynthesis

Wang, Ping; Chen, Hongfu; Qian, Guoliang; Liu, Fengquan

doi:10.1007/s00253-017-8117-8

Cited by 37 publications

(37 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RpfG affects HSAF production at the level of gene expression ( 26 ), therefore we expected LchP to also affect gene expression. To test this prediction, we measured, via quantitative RT-PCR (qRT-PCR), mRNA levels of lafB , the first gene in the HSAF biosynthesis operon ( 17 ). We found the level of the lafB transcript in the lchP mutant to be decreased by >2-fold, compared to the wild type (Figure 3B ).…”

Section: Resultsmentioning

confidence: 99%

“…Lysobacter enzymogenes is a nonpathogenic, plant-associated gammaproteobacterium known for synthesis of the antifungal antibiotic Heat-Stable Antifungal Factor, HSAF ( 11–14 ), which makes it an attractive biological control agent for fungal diseases of agriculturally important plants ( 11 , 12 , 15 ). Therefore, identifying genes and growth conditions that regulate HSAF production has been of significant interest ( 16 , 17 ). Our earlier work revealed that elevated intracellular c-di-GMP levels are detrimental for HSAF production due to lower HSAF biosynthesis gene expression ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Signaling specificity in the c-di-GMP-dependent network regulating antibiotic synthesis in Lysobacter

Han

Huo

et al. 2018

Nucleic Acids Research

Self Cite

140

View full text Add to dashboard Cite

Enzymes controlling intracellular second messengers in bacteria, such as c-di-GMP, often affect some but not other targets. How such specificity is achieved is understood only partially. Here, we present a novel mechanism that enables specific c-di-GMP-dependent inhibition of the antifungal antibiotic production. Expression of the biosynthesis operon for Heat-Stable Antifungal Factor, HSAF, in Lysobacter enzymogenes occurs when the transcription activator Clp binds to two upstream sites. At high c-di-GMP levels, Clp binding to the lower-affinity site is compromised, which is sufficient to decrease gene expression. We identified a weak c-di-GMP phosphodiesterase, LchP, that plays a disproportionately high role in HSAF synthesis due to its ability to bind Clp. Further, Clp binding stimulates phosphodiesterase activity of LchP. An observation of a signaling complex formed by a c-di-GMP phosphodiesterase and a c-di-GMP-binding transcription factor lends support to the emerging paradigm that such signaling complexes are common in bacteria, and that bacteria and eukaryotes employ similar solutions to the specificity problem in second messenger-based signaling systems.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Signaling specificity in the c-di-GMP-dependent network regulating antibiotic synthesis in Lysobacter

Han

Huo

et al. 2018

Nucleic Acids Research

Self Cite

140

View full text Add to dashboard Cite

show abstract

“…This transcriptional factor binds to a 150‐bp region located upstream of the pks/nrps gene, representing the potential promoter of the pks/nrps gene (Wang et al . ).…”

Section: The Regulatory Network In Lysobacter Enzymogenesmentioning

confidence: 97%

The impact of the omics era on the knowledge and use ofLysobacterspecies to control phytopathogenic micro-organisms

2017

View full text Add to dashboard Cite

Omics technologies have had a tremendous impact on underinvestigated genera of plant disease biocontrol agents such as Lysobacter. Strong evidence of the association between Lysobacter spp. and the rhizosphere has been obtained through culture-independent methods, which has also contributed towards highlighting the relationship between Lysobacter abundance and soil suppressiveness. It is conceivable that the role played by Lysobacter spp. in soil suppressiveness is related to their ability to produce an impressive array of lytic enzymes and antibiotics. Indeed, genomics has revealed that biocontrol Lysobacter strains share a vast number of genes involved in antagonism activities, and the molecular pathways underlying how Lysobacter spp. interact with the environment and other micro-organisms have been depicted through transcriptomic analysis. Furthermore, omics technologies shed light on the regulatory pathways governing cell motility and the biosynthesis of antibiotics. Overall, the results achieved so far through omics technologies confirm that the genus Lysobacter is a valuable source of novel biocontrol agents, paving the way for studies aimed at making their application in field conditions more reliable.

show abstract

“…Moreover, HSAF is synthesized by an operon containing more than ten genes in L. enzymogens , wherein lafB (described initially as hsaf pks/nrps ), the first gene of this operon, encodes a hybrid polyketide synthase/nonribosomal peptide synthetase that catalyses the linkage of one ornithine to two polyketide chains during HSAF assembly. This mechanism is unique among most known biosynthesis reactions of natural bacterial products (Chen et al , ; Lou et al , ; Wang et al , ). Together, these unique features render HSAF an ideal candidate for the development of fungicides for crop protection.…”

Section: Introductionmentioning

confidence: 99%

A YajQ‐LysR‐like, cyclic di‐GMP‐dependent system regulating biosynthesis of an antifungal antibiotic in a crop‐protecting bacterium, Lysobacter enzymogenes

Han

Shen

Wang

et al. 2019

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

YajQ, a binding protein of the universal bacterial second messenger cyclic di-GMP (c-di-GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris. In this bacterium, YajQ interacts with the transcription factor LysR. Upon c-di-GMP binding, the whole c-di-GMP-YajQ-LysR complex is found to dissociate from DNA, resulting in virulence gene regulation. Here, we identify a YajQ-LysR-like system in the bacterial biocontrol agent Lysobacter enzymogenes OH11 that secretes an antifungal antibiotic, heat-stable antifungal factor (HSAF) against crop fungal pathogens. We show that the YajQ homologue, CdgL (c-di-GMP receptor interacting with LysR) affects expression of the HSAF biosynthesis operon by interacting with the transcription activator LysR. The CdgL-LysR interaction enhances the apparent affinity of LysR to the promoter region upstream of the HSAF biosynthesis operon, which increases operon expression. Unlike the homologues CdgL (YajQ)-LysR system in X. campestris, we show that c-di-GMP binding to CdgL seems to weaken CdgL-LysR interactions and promote the release of CdgL from the LysR-DNA complex, which leads to decreased expression. Together, this study takes the YajQ-LysR-like system from bacterial pathogens to a crop-protecting bacterium that is able to regulate antifungal HSAF biosynthesis via disassembly of the c-di-GMP receptortranscription activator complex.

show abstract

LetR is a TetR family transcription factor from Lysobacter controlling antifungal antibiotic biosynthesis

Cited by 37 publications

References 30 publications

Signaling specificity in the c-di-GMP-dependent network regulating antibiotic synthesis in Lysobacter

Signaling specificity in the c-di-GMP-dependent network regulating antibiotic synthesis in Lysobacter

The impact of the omics era on the knowledge and use ofLysobacterspecies to control phytopathogenic micro-organisms

A YajQ‐LysR‐like, cyclic di‐GMP‐dependent system regulating biosynthesis of an antifungal antibiotic in a crop‐protecting bacterium, Lysobacter enzymogenes

Contact Info

Product

Resources

About